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Human peripheral blood mononuclear cells transfected with messenger RNA stimulate antigen-specific cytotoxic T-lymphocytes in vitro.
Teufel R, Carralot JP, Scheel B, Probst J, Walter S, Jung G, Hoerr I, Rammensee HG, Pascolo S. Teufel R, et al. Cell Mol Life Sci. 2005 Aug;62(15):1755-62. doi: 10.1007/s00018-005-5067-6. Cell Mol Life Sci. 2005. PMID: 16003494
We show here that fresh peripheral blood mononuclear cells (PBMCs) can be transfected with mRNA by electroporation. Such cells are as efficient as mRNA-transfected Mo-DCs for their ability to activate memory T cells i …
We show here that fresh peripheral blood mononuclear cells (PBMCs) can be transfected with mRNA by electr …
Increased cytotoxic capacity of tumor antigen specific human T cells after in vitro stimulation with IL21 producing dendritic cells.
Turksma AW, Bontkes HJ, Ruizendaal JJ, van den Heuvel H, Scholten KB, Santegoets SJ, de Gruijl TD, Meijer CJ, Hooijberg E. Turksma AW, et al. Hum Immunol. 2013 May;74(5):506-13. doi: 10.1016/j.humimm.2013.01.014. Epub 2013 Jan 29. Hum Immunol. 2013. PMID: 23376456
Monocyte derived dendritic cells (moDC) electroporated with tumor associated antigen derived mRNA can elicit specific T cells against tumor cells in vivo. IL21 has been shown to enhance activation and cytotoxicity in CD8+ T cell …
Monocyte derived dendritic cells (moDC) electroporated with tumor associated antigen derived mRNA can elicit specific …
Induction of antigen-specific cytotoxic T lymphocytes by using monocyte-derived DCs transfected with in vitro-transcribed WT1 or SART1 mRNA.
Narita M, Tochiki N, Saitoh A, Watanabe N, Kaji M, Satoh N, Yamahira A, Nakamura T, Masuko M, Furukawa T, Toba K, Fuse I, Aizawa Y, Takahashi M. Narita M, et al. Med Oncol. 2009 Dec;26(4):429-36. doi: 10.1007/s12032-008-9142-3. Epub 2008 Dec 5. Med Oncol. 2009. PMID: 19058036
To evaluate the usefulness of monocyte-derived dendritic cells transfected with tumor antigen mRNA for dendritic cell-based antitumor immunotherapy, we attempted to generate antigen-specific cytotoxic T cells by priming l …
To evaluate the usefulness of monocyte-derived dendritic cells transfected with tumor antigen mRNA for dendritic cell-b …
Cotransfection of dendritic cells with RNA coding for HER-2/neu and 4-1BBL increases the induction of tumor antigen specific cytotoxic T lymphocytes.
Grünebach F, Kayser K, Weck MM, Müller MR, Appel S, Brossart P. Grünebach F, et al. Cancer Gene Ther. 2005 Sep;12(9):749-56. doi: 10.1038/sj.cgt.7700842. Cancer Gene Ther. 2005. PMID: 15877082
Ribonucleic acid (RNA) transfection of dendritic cells (DCs) was shown to be highly efficient in eliciting CD8+ and CD4+ T-cell responses. We analyzed whether electroporation of DCs with RNA coding for a tumor-associated antigen (TAA) wou …
Ribonucleic acid (RNA) transfection of dendritic cells (DCs) was shown to be highly efficient in eliciting CD8+ and CD4 …
Delivery of tumor-derived RNA for the induction of cytotoxic T-lymphocytes.
Grünebach F, Müller MR, Nencioni A, Brossart P. Grünebach F, et al. Gene Ther. 2003 Mar;10(5):367-74. doi: 10.1038/sj.gt.3301901. Gene Ther. 2003. PMID: 12601391
Dendritic cells (DC) are professional antigen-presenting cells playing a central role in the induction of antigen-specific cytotoxic T-lymphocytes (CTL). ...However, when these approaches were applied to transduce DC with …
Dendritic cells (DC) are professional antigen-presenting cells playing a central role in the induction of antigen …
In vitro induction of myeloid leukemia-specific CD4 and CD8 T cells by CD40 ligand-activated B cells gene modified to express primary granule proteins.
Fujiwara H, Melenhorst JJ, El Ouriaghli F, Kajigaya S, Grube M, Sconocchia G, Rezvani K, Price DA, Hensel NF, Douek DC, Barrett AJ. Fujiwara H, et al. Clin Cancer Res. 2005 Jun 15;11(12):4495-503. doi: 10.1158/1078-0432.CCR-04-2363. Clin Cancer Res. 2005. PMID: 15958635 Free PMC article.
Therefore, we developed a method to induce T-cell responses to PGP protein sequences. We found that gene-transfected antigen-presenting cells efficiently expand functionally competent PGP-specific CD4 and CD8 T cells. ...To generat …
Therefore, we developed a method to induce T-cell responses to PGP protein sequences. We found that gene-transfected antige …
RNA-transfection of gamma/delta T cells with a chimeric antigen receptor or an alpha/beta T-cell receptor: a safer alternative to genetically engineered alpha/beta T cells for the immunotherapy of melanoma.
Harrer DC, Simon B, Fujii SI, Shimizu K, Uslu U, Schuler G, Gerer KF, Hoyer S, Dörrie J, Schaft N. Harrer DC, et al. BMC Cancer. 2017 Aug 17;17(1):551. doi: 10.1186/s12885-017-3539-3. BMC Cancer. 2017. PMID: 28818060 Free PMC article.
All employed T cells were efficiently transfected with the TCR or the CAR. Upon respective stimulation, gamma/delta T cells produced IFNgamma and TNF, but little IL-2 and the zoledronic-acid expanded T cells exceeded MACS-ga …
All employed T cells were efficiently transfected with the TCR or the CAR. Upon respective stimulation, gamma/de …
Induction of cytotoxic T-lymphocyte responses using dendritic cells transfected with hepatocellular carcinoma mRNA.
Peng BG, He Q, Liang LI, Xie BH, Hua YP, Chen ZB, Zhou F. Peng BG, et al. Br J Biomed Sci. 2006;63(3):123-8. doi: 10.1080/09674845.2006.11732731. Br J Biomed Sci. 2006. PMID: 17058712
This study aims to induce an efficient expansion of cytotoxic T-lymphocytes (CTL) from peripheral blood mononuclear cells (PBMCs) using dendritic cells (DC) transfected with hepatocellular carcinoma (HCC) messenger …
This study aims to induce an efficient expansion of cytotoxic T-lymphocytes (CTL) from peripheral blood …
Langerhans-type and monocyte-derived human dendritic cells have different susceptibilities to mRNA electroporation with distinct effects on maturation and activation: implications for immunogenicity in dendritic cell-based immunotherapy.
Chung DJ, Romano E, Pronschinske KB, Shyer JA, Mennecozzi M, St Angelo ET, Young JW. Chung DJ, et al. J Transl Med. 2013 Jul 9;11:166. doi: 10.1186/1479-5876-11-166. J Transl Med. 2013. PMID: 23837662 Free PMC article.
Clinical responses to electroporated moDC vaccines, however, have been suboptimal. Human Langerhans-type DCs (LCs) are the most potent conventional DC subtype for inducing CD8+ cytotoxic T lymphocytes (CTLs) in vitro. We recently demonstrated th …
Clinical responses to electroporated moDC vaccines, however, have been suboptimal. Human Langerhans-type DCs (LCs) are the most poten …
Enhanced induction of telomerase-specific CD4(+) T cells using dendritic cells transfected with RNA encoding a chimeric gene product.
Su Z, Vieweg J, Weizer AZ, Dahm P, Yancey D, Turaga V, Higgins J, Boczkowski D, Gilboa E, Dannull J. Su Z, et al. Cancer Res. 2002 Sep 1;62(17):5041-8. Cancer Res. 2002. PMID: 12208759 Free article.
In this study, we sought to determine whether human DCs transfected with mRNA encoding a chimeric hTERT/lysosome-associated membrane protein (LAMP-1) protein, carrying the endosomal/lysosomal sorting signal of the LAMP-1, are capable of stimulating concomitan …
In this study, we sought to determine whether human DCs transfected with mRNA encoding a chimeric hTERT/lysosome-associated me …
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