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Organotypic Brain Slice Cultures.
Humpel C. Humpel C. Curr Protoc Immunol. 2018 Nov;123(1):e59. doi: 10.1002/cpim.59. Epub 2018 Oct 12. Curr Protoc Immunol. 2018. PMID: 30311739
Ex vivo cell culture models are of particular interest for neurobiologists, as these allow the study of brain cells in an isolated environment. Organotypic brain slice cultures allow growth of three-dimensional brain tissue that conserves the cellular architecture. This un …
Ex vivo cell culture models are of particular interest for neurobiologists, as these allow the study of brain cells in an isolated environme …
Organotypic brain slice cultures: A review.
Humpel C. Humpel C. Neuroscience. 2015 Oct 1;305:86-98. doi: 10.1016/j.neuroscience.2015.07.086. Epub 2015 Aug 5. Neuroscience. 2015. PMID: 26254240 Free PMC article. Review.
While primary dissociated cultures permit a single homogeneous cell population to be studied, there is a clear need to explore the function of brain cells in a three-dimensional system where the main architecture of the cells is preserved. Thus, organotypic brain slice cul …
While primary dissociated cultures permit a single homogeneous cell population to be studied, there is a clear need to explore the function …
A Combination of Heavy Metals and Intracellular Pathway Modulators Induces Alzheimer Disease-like Pathologies in Organotypic Brain Slices.
Korde DS, Humpel C. Korde DS, et al. Biomolecules. 2024 Jan 30;14(2):165. doi: 10.3390/biom14020165. Biomolecules. 2024. PMID: 38397402 Free PMC article.
The present study aims to establish a cost-effective and rapid method to model the two primary pathologies in organotypic brain slices. Coronal hippocampal brain slices (150 m) were generated from postnatal (day 8-10) C57BL6 wild-type mice and cultured for 9 weeks. ...The …
The present study aims to establish a cost-effective and rapid method to model the two primary pathologies in organotypic brain slice …
Melatonin Activates Anti-Inflammatory Features in Microglia in a Multicellular Context: Evidence from Organotypic Brain Slices and HMC3 Cells.
Merlo S, Caruso GI, Korde DS, Khodorovska A, Humpel C, Sortino MA. Merlo S, et al. Biomolecules. 2023 Feb 16;13(2):373. doi: 10.3390/biom13020373. Biomolecules. 2023. PMID: 36830742 Free PMC article.
In the present study, we exploited the highly preserved cytoarchitecture of organotypic brain cultures (OC) to explore the effects of MEL on hippocampal microglia in a 3D context as compared to a single cell type context represented by the human HMC3 cell line. ...
In the present study, we exploited the highly preserved cytoarchitecture of organotypic brain cultures (OC) to explore the effects of …
Organotypic Brain Slices of ADULT Transgenic Mice: A Tool to Study Alzheimer's Disease.
Humpel C. Humpel C. Curr Alzheimer Res. 2019;16(2):172-181. doi: 10.2174/1567205016666181212153138. Curr Alzheimer Res. 2019. PMID: 30543174 Review.
Organotypic brain slice cultures have been used for many decades but were mainly prepared from postnatal (day 8-10) old rats or mice. ...This review will summarize the studies using organotypic brain slice cultures from adult mice to mimic Alzheimer's disease and wi …
Organotypic brain slice cultures have been used for many decades but were mainly prepared from postnatal (day 8-10) old rats or mice. …
Spreading of Beta-Amyloid in Organotypic Mouse Brain Slices and Microglial Elimination and Effects on Cholinergic Neurons.
Moelgg K, Jummun F, Humpel C. Moelgg K, et al. Biomolecules. 2021 Mar 15;11(3):434. doi: 10.3390/biom11030434. Biomolecules. 2021. PMID: 33804246 Free PMC article.
The aim of the present study was to use organotypic brain slices of postnatal day 8-10 mice. Using collagen hydrogels, we applied different Abeta peptides onto brain slices and analyzed spreading as well as glial reactions after eight weeks of incubation. ...
The aim of the present study was to use organotypic brain slices of postnatal day 8-10 mice. Using collagen hydrogels, we applied dif …
Beta-Amyloid Enhances Vessel Formation in Organotypic Brain Slices Connected to Microcontact Prints.
Steiner K, Humpel C. Steiner K, et al. Biomolecules. 2023 Dec 19;14(1):3. doi: 10.3390/biom14010003. Biomolecules. 2023. PMID: 38275744 Free PMC article.
In the present study, we aim to explore the impact of Abeta on the migration of endothelial cells and subsequent vessel formation. We use organotypic brain slices of postnatal day 10 wildtype mice (C57BL/6) and connect them to small microcontact prints ( CPs) of collagen. …
In the present study, we aim to explore the impact of Abeta on the migration of endothelial cells and subsequent vessel formation. We use …
Novel Plasma Biomarkers for Alzheimer's Disease: Insights from Organotypic Brain Slice and Microcontact Printing Techniques.
Yilmaz SN, Steiner K, Marksteiner J, Faserl K, Sarg B, Humpel C. Yilmaz SN, et al. Front Biosci (Landmark Ed). 2025 Mar 24;30(3):36257. doi: 10.31083/FBL36257. Front Biosci (Landmark Ed). 2025. PMID: 40152394 Free article.
In the present study, we will identify novel plasma biomarkers for AD using our well-established organotypic mouse brain slice model connected to microcontact prints. We hypothesize that AD plasma contains factors that affect endothelial cell migration and new vessel forma …
In the present study, we will identify novel plasma biomarkers for AD using our well-established organotypic mouse brain slice model …
Collagen-induced restructuring of astrocytes and vessels in organotypic mouse brain slices.
Steiner E, Wolfgang V, Humpel C. Steiner E, et al. Front Aging. 2026 Aug 20;7:1910447. doi: 10.3389/fragi.2026.1910447. eCollection 2026. Front Aging. 2026. PMID: 42694361 Free PMC article.
In this study, we explored whether collagen can reconstruct astrocytes and brain vessels by using microcontact printing on organotypic mouse brain slices. METHODS: Organotypic brain slices from postnatal days 8-10 were prepared and connected to collagen microcontact …
In this study, we explored whether collagen can reconstruct astrocytes and brain vessels by using microcontact printing on organotypic …
Acute and chronic noradrenergic regulation of neurotrophin messenger RNA expression in rat hippocampus: evidence from lesions and organotypic cultures.
Hutter P, Johansson M, Saria A, Humpel C. Hutter P, et al. Neuroscience. 1996 Jan;70(1):15-29. doi: 10.1016/0306-4522(95)00346-k. Neuroscience. 1996. PMID: 8848120
To study the acute regulation, hippocampal organotypic slice cultures were prepared and neurotrophin messenger RNAs analysed after incubation in different concentrations of noradrenaline. We report that the noradrenergic N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine depleti …
To study the acute regulation, hippocampal organotypic slice cultures were prepared and neurotrophin messenger RNAs analysed after in …
70 results