Alkaloids from roots of Stephania rotunda and their cholinesterase inhibitory activity

Planta Med. 2010 Oct;76(15):1762-4. doi: 10.1055/s-0030-1249814. Epub 2010 Apr 13.

Abstract

In the course of screening plants used in folk medicine as memory enhancers, a 70% ethanolic extract of Stephania rotunda roots showed significant AChE inhibitory activity. Repeated column chromatography led to the isolation of a new protoberberine alkaloid, which we named stepharotudine (1), and seven known compounds (2-8). The chemical structures of the isolated compounds were elucidated based on extensive 1D and 2D NMR spectroscopic data. Compounds 1-8 were investigated in vitro for their anticholinesterase activity using a rat cortex AChE enzyme.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholinesterase / isolation & purification
  • Alkaloids / chemistry
  • Alkaloids / isolation & purification
  • Alkaloids / pharmacology*
  • Animals
  • Berberine Alkaloids / chemistry
  • Berberine Alkaloids / isolation & purification
  • Berberine Alkaloids / pharmacology
  • Brain / enzymology
  • Cholinesterase Inhibitors / chemistry
  • Cholinesterase Inhibitors / isolation & purification
  • Cholinesterase Inhibitors / pharmacology*
  • GPI-Linked Proteins / antagonists & inhibitors
  • GPI-Linked Proteins / isolation & purification
  • Plant Roots / chemistry
  • Rats
  • Stephania / chemistry*

Substances

  • Alkaloids
  • Berberine Alkaloids
  • Cholinesterase Inhibitors
  • GPI-Linked Proteins
  • stepharotudine
  • Acetylcholinesterase
  • Ache protein, rat