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A colorimetric assay for the determination of 4-diphosphocytidyl-2-C-methyl-D-erythritol 4-phosphate synthase activity.
Bernal C, Palacin C, Boronat A, Imperial S. Bernal C, et al. Among authors: imperial s. Anal Biochem. 2005 Feb 1;337(1):55-61. doi: 10.1016/j.ab.2004.10.011. Anal Biochem. 2005. PMID: 15649375
A single-molecule force spectroscopy nanosensor for the identification of new antibiotics and antimalarials.
Sisquella X, de Pourcq K, Alguacil J, Robles J, Sanz F, Anselmetti D, Imperial S, Fernàndez-Busquets X. Sisquella X, et al. Among authors: imperial s. FASEB J. 2010 Nov;24(11):4203-17. doi: 10.1096/fj.10-155507. Epub 2010 Jul 15. FASEB J. 2010. PMID: 20634351
Design of novel ligands of CDP-methylerythritol kinase by mimicking direct protein-protein and solvent-mediated interactions.
Giménez-Oya V, Villacañas O, Obiol-Pardo C, Antolin-Llovera M, Rubio-Martinez J, Imperial S. Giménez-Oya V, et al. Among authors: imperial s. J Mol Recognit. 2011 Jan-Feb;24(1):71-80. doi: 10.1002/jmr.1024. J Mol Recognit. 2011. PMID: 20213667
Mimicking direct protein-protein and solvent-mediated interactions in the CDP-methylerythritol kinase homodimer: a pharmacophore-directed virtual screening approach.
Giménez-Oya V, Villacañas O, Fernàndez-Busquets X, Rubio-Martinez J, Imperial S. Giménez-Oya V, et al. Among authors: imperial s. J Mol Model. 2009 Aug;15(8):997-1007. doi: 10.1007/s00894-009-0458-5. Epub 2009 Feb 7. J Mol Model. 2009. PMID: 19198899
Homology modeling of Mycobacterium tuberculosis 2C-methyl-D-erythritol-4-phosphate cytidylyltransferase, the third enzyme in the MEP pathway for isoprenoid biosynthesis.
Obiol-Pardo C, Cordero A, Rubio-Martinez J, Imperial S. Obiol-Pardo C, et al. Among authors: imperial s. J Mol Model. 2010 Jun;16(6):1061-73. doi: 10.1007/s00894-009-0615-x. Epub 2009 Nov 15. J Mol Model. 2010. PMID: 19916033
Determination of the Rh factor: A practical illustrating the use of the polymerase chain reaction.
Imperial S, Boronat A. Imperial S, et al. Biochem Mol Biol Educ. 2005 Jan;33(1):50-3. doi: 10.1002/bmb.2005.494033010425. Biochem Mol Biol Educ. 2005. PMID: 21638540
Functional and evolutionary analysis of DXL1, a non-essential gene encoding a 1-deoxy-D-xylulose 5-phosphate synthase like protein in Arabidopsis thaliana.
Carretero-Paulet L, Cairó A, Talavera D, Saura A, Imperial S, Rodríguez-Concepción M, Campos N, Boronat A. Carretero-Paulet L, et al. Among authors: imperial s. Gene. 2013 Jul 15;524(1):40-53. doi: 10.1016/j.gene.2012.10.071. Epub 2012 Nov 13. Gene. 2013. PMID: 23154062 Free article.
Cloning and functional characterization of an enzyme from Helicobacter pylori that catalyzes two steps of the methylerythritol phosphate pathway for isoprenoid biosynthesis.
Pérez-Gil J, Bergua M, Boronat A, Imperial S. Pérez-Gil J, et al. Among authors: imperial s. Biochim Biophys Acta. 2010 Sep;1800(9):919-28. doi: 10.1016/j.bbagen.2010.06.008. Epub 2010 Jun 23. Biochim Biophys Acta. 2010. PMID: 20600626
Biosynthesis of isoprenoids in plants: structure of the 2C-methyl-D-erithrytol 2,4-cyclodiphosphate synthase from Arabidopsis thaliana. Comparison with the bacterial enzymes.
Calisto BM, Perez-Gil J, Bergua M, Querol-Audi J, Fita I, Imperial S. Calisto BM, et al. Among authors: imperial s. Protein Sci. 2007 Sep;16(9):2082-8. doi: 10.1110/ps.072972807. Epub 2007 Jul 27. Protein Sci. 2007. PMID: 17660251 Free PMC article.
A spectrophotometric assay for the determination of 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase activity.
Bernal C, Mendez E, Terencio J, Boronat A, Imperial S. Bernal C, et al. Among authors: imperial s. Anal Biochem. 2005 May 15;340(2):245-51. doi: 10.1016/j.ab.2005.01.055. Anal Biochem. 2005. PMID: 15840497
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