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In Vitro Silencing of lncRNA Expression Using siRNAs.
Thijssen MS, Bintz J, Arnes L. Thijssen MS, et al. Methods Mol Biol. 2021;2348:141-156. doi: 10.1007/978-1-0716-1581-2_9. Methods Mol Biol. 2021. PMID: 34160804
RNA interference (RNAi) enables sequence-specific gene silencing and can, in principle, be employed to silence virtually any gene. ...In this chapter we describe the analysis of lncRNA splicing variant expression, including subcell
RNA interference (RNAi) enables sequence-specific gene silencing and can, in principle, be employed to silence v
The gut microbiota metabolite capsiate regulate SLC2A1 expression by targeting HIF-1alpha to inhibit knee osteoarthritis-induced ferroptosis.
Guan Z, Jin X, Guan Z, Liu S, Tao K, Luo L. Guan Z, et al. Aging Cell. 2023 Jun;22(6):e13807. doi: 10.1111/acel.13807. Epub 2023 Mar 8. Aging Cell. 2023. PMID: 36890785 Free PMC article.
Iron and oxidative stress indicators were determined in peripheral blood samples. And then in vivo and in vitro experiments, a surgically destabilized medial meniscus (DMM) mice model was established and treated with CAT or Ferric Inhibitor-1 (Fer-1). ...Moreover, C …
Iron and oxidative stress indicators were determined in peripheral blood samples. And then in vivo and in vitro experiments, a …
Targeting lncRNA DDIT4-AS1 Sensitizes Triple Negative Breast Cancer to Chemotherapy via Suppressing of Autophagy.
Jiang T, Zhu J, Jiang S, Chen Z, Xu P, Gong R, Zhong C, Cheng Y, Sun X, Yi W, Yang J, Zhou W, Cheng Y. Jiang T, et al. Adv Sci (Weinh). 2023 Jun;10(17):e2207257. doi: 10.1002/advs.202207257. Epub 2023 Apr 25. Adv Sci (Weinh). 2023. PMID: 37096846 Free PMC article.
In this study, it is found that the lncRNA, DNA damage inducible transcript 4 antisense RNA1 (DDIT4-AS1), is highly expressed in triple-negative breast cancer (TNBC) cell lines and tissues due to H3K27 acetylation in the promoter region, and promotes the proliferati …
In this study, it is found that the lncRNA, DNA damage inducible transcript 4 antisense RNA1 (DDIT4-AS1), is highly expressed
Comprehensive expression, prognostic and validation analysis of necroptosis-related lncRNAs in esophageal cancer.
Duan X, Hou R, Huang Y, Wang C, Liu L, Du H, Shi J. Duan X, et al. Transl Oncol. 2024 Aug;46:101983. doi: 10.1016/j.tranon.2024.101983. Epub 2024 May 25. Transl Oncol. 2024. PMID: 38797018 Free PMC article.
BACKGROUND: Previous studies have shown that necroptosis-related long noncoding RNA (lncRNA) risk models can be used to predict prognosis and immune infiltration in patients with esophageal cancer. ...Small interfering RNA (siRN
BACKGROUND: Previous studies have shown that necroptosis-related long noncoding RNA (lncRNA) risk models can be …
Long Non-coding RNA MALAT1 Is Depleted With Age in Skeletal Muscle in vivo and MALAT1 Silencing Increases Expression of TGF-beta1 in vitro.
Ruan L, Mendhe B, Parker E, Kent A, Isales CM, Hill WD, McGee-Lawrence M, Fulzele S, Hamrick MW. Ruan L, et al. Front Physiol. 2022 Jan 21;12:742004. doi: 10.3389/fphys.2021.742004. eCollection 2021. Front Physiol. 2022. PMID: 35126169 Free PMC article.
In vitro studies using mouse C2C12 myoblasts demonstrate that MALAT1 silencing using siRNA increases miR-34a expression, consistent with a role for MALAT1 as an inhibitor of miR-34a-5p activity. ...Finally, miR-34a-5p is implicated in tissue fib
In vitro studies using mouse C2C12 myoblasts demonstrate that MALAT1 silencing using siRNA increases miR-34a
High expression of lncRNA SNHG1 in prostate cancer patients and inhibition of SNHG1 suppresses cell proliferation and promotes apoptosis.
Tang Q, Li Z, Han W, Cheng S, Wang Y. Tang Q, et al. Indian J Pathol Microbiol. 2020 Oct-Dec;63(4):575-580. doi: 10.4103/IJPM.IJPM_612_19. Indian J Pathol Microbiol. 2020. PMID: 33154308
OBJECTIVE: This study aimed to investigate the expression of long non-coding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) in prostate cancer (PCa) patients and to assess the effects of SNHG1 on PCa cell proliferation and ap …
OBJECTIVE: This study aimed to investigate the expression of long non-coding RNA (lncRNA) small nucleolar …
In Silico and In Vitro Analysis of lncRNA XIST Reveals a Panel of Possible Lung Cancer Regulators and a Five-Gene Diagnostic Signature.
Katopodis P, Dong Q, Halai H, Fratila CI, Polychronis A, Anikin V, Sisu C, Karteris E. Katopodis P, et al. Cancers (Basel). 2020 Nov 24;12(12):3499. doi: 10.3390/cancers12123499. Cancers (Basel). 2020. PMID: 33255394 Free PMC article.
Long non-coding RNAs (lncRNAs) perform a wide functional repertoire of roles in cell biology, ranging from RNA editing to gene regulation, as well as tumour genesis and tumour progression. ...Differentially expressed genes (DEGs) were determined
Long non-coding RNAs (lncRNAs) perform a wide functional repertoire of roles in cell biology, ranging from RNA editing
LncRNA PEG11as silencing sponges miR-874-3p to alleviate cerebral ischemia stroke via regulating autophagy in vivo and in vitro.
Hu X, Ma F, Cheng Z, Zeng S, Shen R, Li X, Hu J, Jin Z, Cheng J. Hu X, et al. Aging (Albany NY). 2022 Jun 24;14(12):5177-5194. doi: 10.18632/aging.204140. Epub 2022 Jun 24. Aging (Albany NY). 2022. PMID: 35749138 Free PMC article.
Long non-coding RNAs (lncRNAs) are reportedly involved in the regulation of physiological and pathophysiological processes. However, the potential role of lncRNAs in stroke remains largely undefined. Here, RNA-Seq analysis of lncRNAs found that
Long non-coding RNAs (lncRNAs) are reportedly involved in the regulation of physiological and pathophysiological processes. Ho
Silencing of lncRNA CCDC26 Restrains the Growth and Migration of Glioma Cells In Vitro and In Vivo via Targeting miR-203.
Wang S, Hui Y, Li X, Jia Q. Wang S, et al. Oncol Res. 2018 Sep 14;26(8):1143-1154. doi: 10.3727/096504017X14965095236521. Epub 2017 Jun 9. Oncol Res. 2018. PMID: 28600863 Free PMC article.
Here we aimed to investigate the effect of long noncoding RNA (lncRNA) coiled-coil domain-containing 26 (CCDC26) in glioma progression. ...Simultaneously, the expression of miR-203 was found suppressed in glioma tissues and cells lines. Suppress …
Here we aimed to investigate the effect of long noncoding RNA (lncRNA) coiled-coil domain-containing 26 (CCDC26) …
Increased Expression of lncRNA UCA1 and HULC Is Required for Pro-inflammatory Response During LPS Induced Sepsis in Endothelial Cells.
Chen Y, Fu Y, Song YF, Li N. Chen Y, et al. Front Physiol. 2019 May 21;10:608. doi: 10.3389/fphys.2019.00608. eCollection 2019. Front Physiol. 2019. PMID: 31231228 Free PMC article.
RNA interference using siRNAs targeting HULC and UCA1, but not MALAT-1, decreased the expression of IL6, ICAM1, and VCAM1 to endogenous levels. ...Cumulatively, these results suggest that LPS induced in vitro sepsis in endothelial cells and indu
RNA interference using siRNAs targeting HULC and UCA1, but not MALAT-1, decreased the expression of IL6, ICAM1, and VCA
274 results