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Influence of HEK293 metabolism on the production of viral vectors and vaccine.
Petiot E, Cuperlovic-Culf M, Shen CF, Kamen A. Petiot E, et al. Vaccine. 2015 Nov 4;33(44):5974-81. doi: 10.1016/j.vaccine.2015.05.097. Epub 2015 Jun 11. Vaccine. 2015. PMID: 26073013 Review.
Mammalian cell cultures are increasingly used for the production of complex biopharmaceuticals including viral vectors and vaccines. ...Also, we will summarize the current knowledge on HEK293 metabolism indicating remaining issues to addr …
Mammalian cell cultures are increasingly used for the production of complex biopharmaceuticals including viral vectors
Integrating nanoparticle quantification and statistical design of experiments for efficient HIV-1 virus-like particle production in High Five cells.
Puente-Massaguer E, Lecina M, Gòdia F. Puente-Massaguer E, et al. Appl Microbiol Biotechnol. 2020 Feb;104(4):1569-1582. doi: 10.1007/s00253-019-10319-x. Epub 2020 Jan 6. Appl Microbiol Biotechnol. 2020. PMID: 31907573 Free PMC article.
To date, most of the studies dealing with VLP production by recombinant baculovirus infection utilize indirect detection or quantification techniques that hinder the appropriate characterization of the process and product. ...The synergies between CCI, MOI, and TOH …
To date, most of the studies dealing with VLP production by recombinant baculovirus infection utilize indirect detection or quantific …
Yeast virus-derived stimulator of the innate immune system augments the efficacy of virus vector-based immunotherapy.
Claudepierre MC, Hortelano J, Schaedler E, Kleinpeter P, Geist M, Remy-Ziller C, Brandely R, Tosch C, Laruelle L, Jawhari A, Menguy T, Marchand JB, Romby P, Schultz P, Hartmann G, Rooke R, Bonnefoy JY, Preville X, Rittner K. Claudepierre MC, et al. J Virol. 2014 May;88(10):5242-55. doi: 10.1128/JVI.03819-13. Epub 2014 Feb 26. J Virol. 2014. PMID: 24574403 Free PMC article.
To identify novel stimulators of the innate immune system, we constructed a panel of eight HEK293 cell lines double positive for human Toll-like receptors (TLRs) and an NF-kappaB-inducible reporter gene. ...We suggest indirect mechanisms of activation of these cell types b …
To identify novel stimulators of the innate immune system, we constructed a panel of eight HEK293 cell lines double positive for huma …