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Functional modulation of Escherichia coli RNA polymerase.
Ishihama A. Ishihama A. Annu Rev Microbiol. 2000;54:499-518. doi: 10.1146/annurev.micro.54.1.499. Annu Rev Microbiol. 2000. PMID: 11018136 Review.
The overall differentiated state of approximately 2000 molecules of the RNA polymerase in a single cell can be estimated after measurement of both the intracellular concentrations and the RNA polymerase-binding affinities for all seven species of the sigma subunit and 100- …
The overall differentiated state of approximately 2000 molecules of the RNA polymerase in a single cell can be estimated after measur …
[Ambisense RNA genome of rice stripe virus].
Hamamatsu C, Ishihama A. Hamamatsu C, et al. Uirusu. 1994 Jun;44(1):19-25. doi: 10.2222/jsv.44.19. Uirusu. 1994. PMID: 7856115 Review. Japanese. No abstract available.
Ribosome modulation factor: stationary growth phase-specific inhibitor of ribosome functions from Escherichia coli.
Wada A, Igarashi K, Yoshimura S, Aimoto S, Ishihama A. Wada A, et al. Biochem Biophys Res Commun. 1995 Sep 14;214(2):410-7. doi: 10.1006/bbrc.1995.2302. Biochem Biophys Res Commun. 1995. PMID: 7677746
RMF either purified from stationary-phase ribosomes or synthesized by a chemical method was examined for its functions. By adding either natural or synthetic RMF to 70S ribosomes prepared from both exponentially growing and stationary phase cells, 100S ribosome dimers were …
RMF either purified from stationary-phase ribosomes or synthesized by a chemical method was examined for its functions. By adding eit …
Regulatory role of XynR (YagI) in catabolism of xylonate in Escherichia coli K-12.
Shimada T, Momiyama E, Yamanaka Y, Watanabe H, Yamamoto K, Ishihama A. Shimada T, et al. FEMS Microbiol Lett. 2017 Dec 1;364(22). doi: 10.1093/femsle/fnx220. FEMS Microbiol Lett. 2017. PMID: 29087459
As an initial attempt to make a breakthrough for understanding the regulatory roles of cryptic phage-encoded TFs, we tried to identify the regulatory function of CP4-6 cryptic prophage-encoded YagI with unknown function. After SELEX screening, YagI was found to bind mainly …
As an initial attempt to make a breakthrough for understanding the regulatory roles of cryptic phage-encoded TFs, we tried to identif …
Cross-regulation between two common ancestral response regulators, HprR and CusR, in Escherichia coli.
Urano H, Yoshida M, Ogawa A, Yamamoto K, Ishihama A, Ogasawara H. Urano H, et al. Microbiology. 2017 Feb;163(2):243-252. doi: 10.1099/mic.0.000410. Epub 2017 Mar 9. Microbiology. 2017. PMID: 27983483
Towards understanding this unique regulation system, in this study we analysed the interplay between HprR and CusR with respect to transcription of hiuH, a regulatory target of HprR, and cusC, a target of CusR. ...This is a new type of transcription regulatio …
Towards understanding this unique regulation system, in this study we analysed the interplay between HprR and CusR with respect to transcrip …
Strength and Regulation of Seven rRNA Promoters in Escherichia coli.
Maeda M, Shimada T, Ishihama A. Maeda M, et al. PLoS One. 2015 Dec 30;10(12):e0144697. doi: 10.1371/journal.pone.0144697. eCollection 2015. PLoS One. 2015. PMID: 26717514 Free PMC article.
Using this modified pGRS system, the promoter activity of each rrn operon was determined by measuring the rrn promoter-directed GFP and the reference promoter-directed RFP fluorescence, both encoded by a single and the same vector. ...These findings altogether indicate tha …
Using this modified pGRS system, the promoter activity of each rrn operon was determined by measuring the rrn promoter-directed GFP and the …
Cooperative regulation of the common target genes between H₂O₂-sensing YedVW and Cu²⁺-sensing CusSR in Escherichia coli.
Urano H, Umezawa Y, Yamamoto K, Ishihama A, Ogasawara H. Urano H, et al. Microbiology. 2015 Apr;161(Pt 4):729-38. doi: 10.1099/mic.0.000026. Epub 2015 Jan 7. Microbiology. 2015. PMID: 25568260
Using a reporter assay system, we found that transcription of hiuH, encoding 5-hydroxyisourate hydrolase, was induced at high concentrations of either Cu(2+) or H₂O₂. ...Taken together, we concluded that YedVW and CusSR formed a unique cooperative TCS pair by recogn …
Using a reporter assay system, we found that transcription of hiuH, encoding 5-hydroxyisourate hydrolase, was induced at high concent …
Role for σ38 in prolonged survival of Escherichia coli in Drosophila melanogaster.
Shiratsuchi A, Shimamoto N, Nitta M, Tuan TQ, Firdausi A, Gawasawa M, Yamamoto K, Ishihama A, Nakanishi Y. Shiratsuchi A, et al. J Immunol. 2014 Jan 15;192(2):666-75. doi: 10.4049/jimmunol.1300968. Epub 2013 Dec 13. J Immunol. 2014. PMID: 24337747
The promoter-recognizing subunit σ of bacterial RNA polymerase plays a major role in the selection of genes to be transcribed. ...We found a transient increase of σ(38) when E. coli was injected into the hemocoel of Drosophila melanogaster. The loss of σ(38) made E. …
The promoter-recognizing subunit σ of bacterial RNA polymerase plays a major role in the selection of genes to be transcribed. ...We …
Identification of the set of genes, including nonannotated morA, under the direct control of ModE in Escherichia coli.
Kurata T, Katayama A, Hiramatsu M, Kiguchi Y, Takeuchi M, Watanabe T, Ogasawara H, Ishihama A, Yamamoto K. Kurata T, et al. J Bacteriol. 2013 Oct;195(19):4496-505. doi: 10.1128/JB.00304-13. Epub 2013 Aug 2. J Bacteriol. 2013. PMID: 23913318 Free PMC article.
ModE is activated by binding molybdate and acts as both an activator and a repressor. By genomic systematic evolution of ligands by exponential enrichment (SELEX) screening and promoter reporter assays, we have identified a total of nine operons, including the hithe …
ModE is activated by binding molybdate and acts as both an activator and a repressor. By genomic systematic evolution of ligands by e …
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