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Monitoring of HIV-1 envelope-mediated membrane fusion using modified split green fluorescent proteins.
Wang J, Kondo N, Long Y, Iwamoto A, Matsuda Z. Wang J, et al. J Virol Methods. 2009 Nov;161(2):216-22. doi: 10.1016/j.jviromet.2009.06.017. Epub 2009 Jun 25. J Virol Methods. 2009. PMID: 19559731 Free PMC article.
A simple, cell-based, membrane fusion assay system that uses split green fluorescent proteins (spGFPs) as an indicator was developed. ...
A simple, cell-based, membrane fusion assay system that uses split green fluorescent proteins (spGFPs) as an indicator was developed.
Novel immunodominant peptide presentation strategy: a featured HLA-A*2402-restricted cytotoxic T-lymphocyte epitope stabilized by intrachain hydrogen bonds from severe acute respiratory syndrome coronavirus nucleocapsid protein.
Liu J, Wu P, Gao F, Qi J, Kawana-Tachikawa A, Xie J, Vavricka CJ, Iwamoto A, Li T, Gao GF. Liu J, et al. J Virol. 2010 Nov;84(22):11849-57. doi: 10.1128/JVI.01464-10. Epub 2010 Sep 15. J Virol. 2010. PMID: 20844028 Free PMC article.
Here, a severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid (N) protein-derived CTL epitope, N1 (QFKDNVILL), restricted by HLA-A*2402 was identified by a series of in vitro studies, including a computer-assisted algorithm for predicti …
Here, a severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid (N) protein-derived CTL epitope, N1 (QFKDNVILL), restri …
Co-expression of foreign proteins tethered to HIV-1 envelope glycoprotein on the cell surface by introducing an intervening second membrane-spanning domain.
Wang H, Li X, Nakane S, Liu S, Ishikawa H, Iwamoto A, Matsuda Z. Wang H, et al. PLoS One. 2014 May 7;9(5):e96790. doi: 10.1371/journal.pone.0096790. eCollection 2014. PLoS One. 2014. PMID: 24804933 Free PMC article.
To overcome this problem, here we linked a tag protein called HaloTag at the C-terminus of HIV-1 Env. To relocate HaloTag to the cell surface, we introduced a second membrane-spanning domain (MSD) between Env and HaloTag. ...The surface level of Env can be estimated …
To overcome this problem, here we linked a tag protein called HaloTag at the C-terminus of HIV-1 Env. To relocate HaloTag to the cell …
Inefficient enhancement of viral infectivity and CD4 downregulation by human immunodeficiency virus type 1 Nef from Japanese long-term nonprogressors.
Tobiume M, Takahoko M, Yamada T, Tatsumi M, Iwamoto A, Matsuda M. Tobiume M, et al. J Virol. 2002 Jun;76(12):5959-65. doi: 10.1128/jvi.76.12.5959-5965.2002. J Virol. 2002. PMID: 12021328 Free PMC article.
The patient-derived nef alleles were subcloned into a nef-defective infectious HIV-1 molecular clone and an expression vector. ...Third, all nef alleles from LTNPs, progressors, and ACs reduced the cell surface expression of major histocompatibility complex class I to a
The patient-derived nef alleles were subcloned into a nef-defective infectious HIV-1 molecular clone and an expression vector. ...Thi …
Conformational changes of the HIV-1 envelope protein during membrane fusion are inhibited by the replacement of its membrane-spanning domain.
Kondo N, Miyauchi K, Meng F, Iwamoto A, Matsuda Z. Kondo N, et al. J Biol Chem. 2010 May 7;285(19):14681-8. doi: 10.1074/jbc.M109.067090. Epub 2010 Mar 2. J Biol Chem. 2010. PMID: 20197275 Free PMC article.
To help understand the dynamic nature of membrane fusion induced by the human immunodeficiency virus-1 (HIV-1) envelope protein, we developed a new cell-based real-time assay system employing a pair of novel reporter proteins. ...Use of this reporter together with …
To help understand the dynamic nature of membrane fusion induced by the human immunodeficiency virus-1 (HIV-1) envelope protein, we develope …
Thermus thermophilus-derived protein tags that aid in preparation of insoluble viral proteins.
Kondo N, Ebihara A, Ru H, Kuramitsu S, Iwamoto A, Rao Z, Matsuda Z. Kondo N, et al. Anal Biochem. 2009 Feb 15;385(2):278-85. doi: 10.1016/j.ab.2008.10.050. Epub 2008 Nov 19. Anal Biochem. 2009. PMID: 19084492
From this group, we identified a T. th. tag protein that expressed Vpr in a soluble form. Furthermore, two T. th. tag proteins, including the identified one, were found to solubilize the extremely insoluble membrane-spanning domain of the envelope protein of HIV-1. …
From this group, we identified a T. th. tag protein that expressed Vpr in a soluble form. Furthermore, two T. th. tag proteins …
Functional analysis of HIV-1 vif genes derived from Japanese long-term nonprogressors and progressors for AIDS.
Sakurai A, Jere A, Yoshida A, Yamada T, Iwamoto A, Adachi A, Fujita M. Sakurai A, et al. Microbes Infect. 2004 Jul;6(9):799-805. doi: 10.1016/j.micinf.2004.04.005. Microbes Infect. 2004. PMID: 15374001
We constructed a basic HIV-1 infectious clone, which facilitated the incorporation and evaluation of vif from infected individuals. ...There was one exception in which all the clones examined had inactive vif, suggesting a probable association of inactive vif with t …
We constructed a basic HIV-1 infectious clone, which facilitated the incorporation and evaluation of vif from infected individuals. . …
Antibody-dependent cellular cytotoxicity via humoral immune epitope of Nef protein expressed on cell surface.
Yamada T, Watanabe N, Nakamura T, Iwamoto A. Yamada T, et al. J Immunol. 2004 Feb 15;172(4):2401-6. doi: 10.4049/jimmunol.172.4.2401. J Immunol. 2004. PMID: 14764710
Addition of a specific synthetic peptide (peptide 31:FLKEKGGLE) corresponding to the Nef epitope reduced cell lysis to approximately 50%. These results suggest that PBMCs of HIV-1-infected patients may exert ADCC via anti-Nef Abs in the patients' own plasma and serve as …
Addition of a specific synthetic peptide (peptide 31:FLKEKGGLE) corresponding to the Nef epitope reduced cell lysis to approximately …
Significant reductions in Gag-protease-mediated HIV-1 replication capacity during the course of the epidemic in Japan.
Nomura S, Hosoya N, Brumme ZL, Brockman MA, Kikuchi T, Koga M, Nakamura H, Koibuchi T, Fujii T, Carlson JM, Heckerman D, Kawana-Tachikawa A, Iwamoto A, Miura T. Nomura S, et al. J Virol. 2013 Feb;87(3):1465-76. doi: 10.1128/JVI.02122-12. Epub 2012 Nov 14. J Virol. 2013. PMID: 23152532 Free PMC article.
As a result, the functional properties of HIV-1 isolates from earlier in the epidemic may differ from those of isolates from later stages. ...Viral replication capacity was examined by infecting a long terminal repeat-driven green fluorescent protein-reporter T cell …
As a result, the functional properties of HIV-1 isolates from earlier in the epidemic may differ from those of isolates from later st …
The V4 and V5 Variable Loops of HIV-1 Envelope Glycoprotein Are Tolerant to Insertion of Green Fluorescent Protein and Are Useful Targets for Labeling.
Nakane S, Iwamoto A, Matsuda Z. Nakane S, et al. J Biol Chem. 2015 Jun 12;290(24):15279-91. doi: 10.1074/jbc.M114.628610. Epub 2015 Apr 24. J Biol Chem. 2015. PMID: 25911103 Free PMC article.
We performed insertional mutagenesis using a GFP variant, GFPOPT, placed into the variable loops of HXB2 gp120. This allowed us to evaluate the current structural models and to simultaneously generate a GFP-tagged HIV-1 Env, which was useful for image analyses. ...O …
We performed insertional mutagenesis using a GFP variant, GFPOPT, placed into the variable loops of HXB2 gp120. This allowed us to ev …
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