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The human Golgi protein TMEM165 transports calcium and manganese in yeast and bacterial cells
Stribny J, et al. J Biol Chem 2020. PMID 32047108 Free article.
Using bacterial cells loaded with the fluorescent Fura-2 probe, we further obtained direct biochemical evidence that TMEM165 mediates Ca2+ and Mn2+ influxes. ...
Using bacterial cells loaded with the fluorescent Fura-2 probe, we further obtained direct biochemical evidence that TMEM165 mediates …
Leptin-mediated differential regulation of microsomal triglyceride transfer protein in the intestine and liver affects plasma lipids
Iqbal J, et al. J Biol Chem 2020. PMID 32047110 Free article.
Leptin decreased MTP expression in differentiated intestinal Caco-2 cells, but increased expression in hepatic Huh7 cells. Similarly, acute and chronic leptin treatment of chow diet-fed wildtype mice decreased MTP expression in the intestine, increased it in the liver, and lowered plasma triglyceride levels. ...
Leptin decreased MTP expression in differentiated intestinal Caco-2 cells, but increased expression in hepatic Huh7 cells. Similarly, …
Identification of a selective manganese ionophore that enables non-lethal quantification of cellular manganese
Horning KJ, et al. J Biol Chem 2020. PMID 32047113 Free article.
Use of the MESMER assay versus a comparable detergent lysis-based assay, cellular Fura-2 Mn extraction assay (CFMEA), reduced the number of cells and materials required for performing a similar but cell lethality-based experiment to 25% of the normally required sample size. ...
Use of the MESMER assay versus a comparable detergent lysis-based assay, cellular Fura-2 Mn extraction assay (CFMEA), reduced the num …
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