A specific molecular beacon probe for the detection of human prostate cancer cells

Bioorg Med Chem Lett. 2012 Jun 1;22(11):3632-8. doi: 10.1016/j.bmcl.2012.04.055. Epub 2012 Apr 19.

Abstract

The small-molecule, water-soluble molecular beacon probe 1 is hydrolyzed by the lysate and living cells of human prostate cancer cell lines (LNCaP), resulting in strong green fluorescence. In contrast, probe 1 does not undergo significant hydrolysis in either the lysate or living cells of human nontumorigenic prostate cells (RWPE-1). These results, corroborated by UV-Vis spectroscopy and fluorescent microscopy, reveal that probe 1 is a sensitive and specific fluorogenic and chromogenic sensor for the detection of human prostate cancer cells among nontumorigenic prostate cells and that carboxylesterase activity is a specific biomarker for human prostate cancer cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / metabolism*
  • Carboxylesterase / metabolism
  • Cell Line, Tumor
  • Fluorescent Dyes / chemistry*
  • Humans
  • Hydrogen Bonding
  • Hydrolysis
  • Magnetic Resonance Spectroscopy
  • Male
  • Microscopy, Fluorescence
  • Molecular Conformation
  • Molecular Probes / chemistry*
  • Prostatic Neoplasms / diagnosis
  • Prostatic Neoplasms / enzymology*
  • Prostatic Neoplasms / pathology
  • Spectrophotometry, Ultraviolet
  • Temperature

Substances

  • Biomarkers
  • Fluorescent Dyes
  • Molecular Probes
  • Carboxylesterase