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High-level production of tetraacetyl phytosphingosine (TAPS) by combined genetic engineering of sphingoid base biosynthesis and L-serine availability in the non-conventional yeast Pichia ciferrii.
Schorsch C, Köhler T, Andrea H, Boles E. Schorsch C, et al. Metab Eng. 2012 Mar;14(2):172-84. doi: 10.1016/j.ymben.2011.12.002. Epub 2011 Dec 10. Metab Eng. 2012. PMID: 22178746
Draft genome sequence of Wickerhamomyces ciferrii NRRL Y-1031 F-60-10.
Schneider J, Andrea H, Blom J, Jaenicke S, Rückert C, Schorsch C, Szczepanowski R, Farwick M, Goesmann A, Pühler A, Schaffer S, Tauch A, Köhler T, Brinkrolf K. Schneider J, et al. Eukaryot Cell. 2012 Dec;11(12):1582-3. doi: 10.1128/EC.00258-12. Eukaryot Cell. 2012. PMID: 23193139 Free PMC article.
Metabolic engineering of the non-conventional yeast Pichia ciferrii for production of rare sphingoid bases.
Börgel D, van den Berg M, Hüller T, Andrea H, Liebisch G, Boles E, Schorsch C, van der Pol R, Arink A, Boogers I, van der Hoeven R, Korevaar K, Farwick M, Köhler T, Schaffer S. Börgel D, et al. Metab Eng. 2012 Jul;14(4):412-26. doi: 10.1016/j.ymben.2012.03.003. Epub 2012 Mar 18. Metab Eng. 2012. PMID: 22449569
Production of tetraacetyl phytosphingosine (TAPS) in Wickerhamomyces ciferrii is catalyzed by acetyltransferases Sli1p and Atf2p.
Ter Veld F, Wolff D, Schorsch C, Köhler T, Boles E, Poetsch A. Ter Veld F, et al. Appl Microbiol Biotechnol. 2013 Oct;97(19):8537-46. doi: 10.1007/s00253-012-4670-3. Epub 2013 Jan 15. Appl Microbiol Biotechnol. 2013. PMID: 23318835
Combined application of targeted and untargeted proteomics identifies distinct metabolic alterations in the tetraacetylphytosphingosine (TAPS) producing yeast Wickerhamomyces ciferrii.
Wolff D, ter Veld F, Köhler T, Poetsch A. Wolff D, et al. J Proteomics. 2013 Apr 26;82:274-87. doi: 10.1016/j.jprot.2013.03.002. Epub 2013 Mar 14. J Proteomics. 2013. PMID: 23500128
Role of Cdc42-Cla4 interaction in the pheromone response of Saccharomyces cerevisiae.
Heinrich M, Köhler T, Mösch HU. Heinrich M, et al. Eukaryot Cell. 2007 Feb;6(2):317-27. doi: 10.1128/EC.00102-06. Epub 2006 Dec 22. Eukaryot Cell. 2007. PMID: 17189484 Free PMC article.
Dual role of the Saccharomyces cerevisiae TEA/ATTS family transcription factor Tec1p in regulation of gene expression and cellular development.
Köhler T, Wesche S, Taheri N, Braus GH, Mösch HU. Köhler T, et al. Eukaryot Cell. 2002 Oct;1(5):673-86. doi: 10.1128/ec.1.5.673-686.2002. Eukaryot Cell. 2002. PMID: 12455687 Free PMC article.
High-resolution analysis of gut environment and bacterial microbiota reveals functional compartmentation of the gut in wood-feeding higher termites (Nasutitermes spp.).
Köhler T, Dietrich C, Scheffrahn RH, Brune A. Köhler T, et al. Appl Environ Microbiol. 2012 Jul;78(13):4691-701. doi: 10.1128/AEM.00683-12. Epub 2012 Apr 27. Appl Environ Microbiol. 2012. PMID: 22544239 Free PMC article.
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