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Rapid down-regulation of testicular androgen biosynthesis at increased environmental temperature is due to cytochrome P450c17 (CYP17) thermolability in Leydig cells, but not in endoplasmic reticulum membranes.
Kühn-Velten WN. Kühn-Velten WN. Exp Clin Endocrinol Diabetes. 1996;104(3):243-9. doi: 10.1055/s-0029-1211449. Exp Clin Endocrinol Diabetes. 1996. PMID: 8817242
Characterization of the hydrophobic interaction of steroids with endoplasmic reticulum membranes by quenching of 6,8(14)-bis-dehydro-17 alpha-hydroxyprogesterone fluorescence.
Kühn-Velten WN, Kempfle MA. Kühn-Velten WN, et al. Biochim Biophys Acta. 1993 Feb 9;1145(2):185-90. doi: 10.1016/0005-2736(93)90287-a. Biochim Biophys Acta. 1993. PMID: 8431450
Novel connections between NADPH-induced lipid peroxidation and cytochrome P450 inactivation, and antioxidant and enzyme protective properties of estradiol in gonadal membranes.
Kühn-Velten WN, Pippirs U. Kühn-Velten WN, et al. Free Radic Res. 1997 Feb;26(2):125-33. doi: 10.3109/10715769709097791. Free Radic Res. 1997. PMID: 9257124
Improvement of cytochrome P450c17 catalytic processivity as a novel mechanism to attenuate hormonal consequences of enzyme decay in the testes after a gonadotropin surge.
Kühn-Velten WN. Kühn-Velten WN. Eur J Endocrinol. 1997 Apr;136(4):438-43. doi: 10.1530/eje.0.1360438. Eur J Endocrinol. 1997. PMID: 9150706
Effects of compatible solutes on mammalian cytochrome P450 stability.
Kühn-Velten WN. Kühn-Velten WN. Z Naturforsch C J Biosci. 1997 Jan-Feb;52(1-2):132-5. Z Naturforsch C J Biosci. 1997. PMID: 9090073
Protein phosphorylation changes ligand-binding efficiency of cytochrome P450c17 (CYP17) and accelerates its proteolytic degradation: putative relevance for hormonal regulation of CYP17 activity.
Löhr JB, Kühn-Velten WN. Löhr JB, et al. Biochem Biophys Res Commun. 1997 Feb 13;231(2):403-8. doi: 10.1006/bbrc.1997.6113. Biochem Biophys Res Commun. 1997. PMID: 9070288
Ligand dependence of cytochrome P450c17 protection against proteolytic inactivation: structural, methodological and functional implications.
Kühn-Velten WN, Löhr JB. Kühn-Velten WN, et al. FEBS Lett. 1996 Jun 10;388(1):21-5. doi: 10.1016/0014-5793(96)00466-8. FEBS Lett. 1996. PMID: 8654581
Norharman (beta-carboline) as a potent inhibitory ligand for steroidogenic cytochromes P450 (CYP11 and CYP17).
Kühn-Velten WN. Kühn-Velten WN. Eur J Pharmacol. 1993 Nov 30;250(1):R1-3. doi: 10.1016/0014-2999(93)90648-2. Eur J Pharmacol. 1993. PMID: 8119304
Tetrahydroisoquinoline alkaloids mimic direct but not receptor-mediated inhibitory effects of estrogens and phytoestrogens on testicular endocrine function. Possible significance for Leydig cell insufficiency in alcohol addiction.
Stammel W, Thomas H, Staib W, Kühn-Velten WN. Stammel W, et al. Life Sci. 1991;49(18):1319-29. doi: 10.1016/0024-3205(91)90196-i. Life Sci. 1991. PMID: 1921648
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