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Initiation of encephalomyocarditis virus RNA translation: the authentic initiation site is not selected by a scanning mechanism.
Kaminski A, Howell MT, Jackson RJ. Kaminski A, et al. EMBO J. 1990 Nov;9(11):3753-9. EMBO J. 1990. PMID: 2170120 Free PMC article.
In order to assess the role of ribosome scanning in the selection of the correct initiation site, transcripts of a construct comprising the viral 5' noncoding sequence fused to a reporter gene were translated in vitro and the relative frequency of initiation at thes …
In order to assess the role of ribosome scanning in the selection of the correct initiation site, transcripts of a construct comprisi …
Mechanism of initiation site selection promoted by the human rhinovirus 2 internal ribosome entry site.
Kaminski A, Pöyry TA, Skene PJ, Jackson RJ. Kaminski A, et al. J Virol. 2010 Jul;84(13):6578-89. doi: 10.1128/JVI.00123-10. Epub 2010 Apr 28. J Virol. 2010. PMID: 20427535 Free PMC article.
Translation initiation site usage on the human rhinovirus 2 internal ribosome entry site (IRES) has been examined in a mixed reticulocyte lysate/HeLa cell extract system. ...A single residue was inserted in the apical loop to put AUG-576 in frame with AUG-611, and i …
Translation initiation site usage on the human rhinovirus 2 internal ribosome entry site (IRES) has been examined in a mixed reticulo …
The mechanism of an exceptional case of reinitiation after translation of a long ORF reveals why such events do not generally occur in mammalian mRNA translation.
Pöyry TA, Kaminski A, Connell EJ, Fraser CS, Jackson RJ. Pöyry TA, et al. Genes Dev. 2007 Dec 1;21(23):3149-62. doi: 10.1101/gad.439507. Genes Dev. 2007. PMID: 18056426 Free PMC article.
The upstream cistron encodes a 75-kDa major capsid protein precursor (pre-VP1), and the downstream cistron a 10-kDa minor capsid protein. ...These results suggest a mechanism in which some of the eIF3/40S complexes formed during disassembly of post-terminatio …
The upstream cistron encodes a 75-kDa major capsid protein precursor (pre-VP1), and the downstream cistron a 10-kDa minor caps …
The influence of viral coding sequences on pestivirus IRES activity reveals further parallels with translation initiation in prokaryotes.
Fletcher SP, Ali IK, Kaminski A, Digard P, Jackson RJ. Fletcher SP, et al. RNA. 2002 Dec;8(12):1558-71. RNA. 2002. PMID: 12515388 Free PMC article.
Classical swine fever virus (CSFV) is a member of the pestivirus family, which shares many features in common with hepatitis C virus (HCV). ...Although maximum activity was observed in each case with the homologous combination of coding region and 5' UTR, the heterologous …
Classical swine fever virus (CSFV) is a member of the pestivirus family, which shares many features in common with hepatitis C virus …
What determines whether mammalian ribosomes resume scanning after translation of a short upstream open reading frame?
Pöyry TA, Kaminski A, Jackson RJ. Pöyry TA, et al. Genes Dev. 2004 Jan 1;18(1):62-75. doi: 10.1101/gad.276504. Epub 2003 Dec 30. Genes Dev. 2004. PMID: 14701882 Free PMC article.
If the 5'-proximal AUG triplet in a mammalian mRNA is followed by a short open reading frame (sORF), a significant fraction of ribosomes resume scanning after termination of sORF translation, and reinitiate at a downstream AUG. ...A critical tes …
If the 5'-proximal AUG triplet in a mammalian mRNA is followed by a short open reading frame (sORF), a significant frac …
The influence of viral coding sequences on the efficiency of internal initiation of translation of cardiovirus RNAs.
Hunt SL, Kaminski A, Jackson RJ. Hunt SL, et al. Virology. 1993 Dec;197(2):801-7. doi: 10.1006/viro.1993.1661. Virology. 1993. PMID: 8249303
Therefore, a variety of constructs derived from encephalomyocarditis virus and Theiler's murine encephalomyelitis virus retaining various lengths of viral coding sequence were translated in rabbit reticulocyte lysates. ...These results suggest a possible explanation …
Therefore, a variety of constructs derived from encephalomyocarditis virus and Theiler's murine encephalomyelitis virus retaining var …
Defective point mutants of the encephalomyocarditis virus internal ribosome entry site can be complemented in trans.
Van Der Velden A, Kaminski A, Jackson RJ, Belsham GJ. Van Der Velden A, et al. Virology. 1995 Dec 1;214(1):82-90. doi: 10.1006/viro.1995.9952. Virology. 1995. PMID: 8525641
These mutants were constructed into a dicistronic mRNA expression plasmid and the activities of the mutant IRES elements were determined using the vaccinia virus/T7 RNA polymerase transient expression system in vivo. ...The results are consistent with a model in whi …
These mutants were constructed into a dicistronic mRNA expression plasmid and the activities of the mutant IRES elements were determi …
The polypyrimidine tract binding protein (PTB) requirement for internal initiation of translation of cardiovirus RNAs is conditional rather than absolute.
Kaminski A, Jackson RJ. Kaminski A, et al. RNA. 1998 Jun;4(6):626-38. doi: 10.1017/s1355838298971898. RNA. 1998. PMID: 9622122 Free PMC article.
With a wild-type EMCV IRES (which has a bulge of 6 As), translation is effectively independent of PTB provided the IRES is driving the synthesis of EMCV viral polyprotein. ...Intermediate levels of PTB dependence are seen with a 7A bulge IRES driving viral po …
With a wild-type EMCV IRES (which has a bulge of 6 As), translation is effectively independent of PTB provided the IRES is dri …
Unique features of internal initiation of hepatitis C virus RNA translation.
Reynolds JE, Kaminski A, Kettinen HJ, Grace K, Clarke BE, Carroll AR, Rowlands DJ, Jackson RJ. Reynolds JE, et al. EMBO J. 1995 Dec 1;14(23):6010-20. doi: 10.1002/j.1460-2075.1995.tb00289.x. EMBO J. 1995. PMID: 8846793 Free PMC article.
The question of whether hepatitis C virus (HCV) RNA is translated by a mechanism of internal ribosome entry has been examined by testing whether insertion of HCV sequences between the two cistrons of a dicistronic mRNA promotes translation of the downstream cistron …
The question of whether hepatitis C virus (HCV) RNA is translated by a mechanism of internal ribosome entry has been examined by test …
Internal initiation of translation of hepatitis C virus RNA: the ribosome entry site is at the authentic initiation codon.
Reynolds JE, Kaminski A, Carroll AR, Clarke BE, Rowlands DJ, Jackson RJ. Reynolds JE, et al. RNA. 1996 Sep;2(9):867-78. RNA. 1996. PMID: 8809014 Free PMC article.
Although the first, 5'-proximal, of these is absolutely conserved, a mutational analysis showed that it is not a functional initiation codon. ...
Although the first, 5'-proximal, of these is absolutely conserved, a mutational analysis showed that it is not a functional in …
348 results
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