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Human carbonic anhydrase-8 AAV8 gene therapy inhibits nerve growth factor signaling producing prolonged analgesia and anti-hyperalgesia in mice.
Zhuang GZ, Upadhyay U, Tong X, Kang Y, Erasso DM, Fu ES, Sarantopoulos KD, Martin ER, Wiltshire T, Diatchenko L, Smith SB, Maixner W, Levitt RC. Zhuang GZ, et al. Among authors: kang y. Gene Ther. 2018 Jul;25(4):297-311. doi: 10.1038/s41434-018-0018-7. Epub 2018 Apr 24. Gene Ther. 2018. PMID: 29789638 Free PMC article.
Profound analgesia is associated with a truncated peptide resulting from tissue specific alternative splicing of DRG CA8-204 regulated by an exon-level cis-eQTL.
Upadhyay U, Zhuang GZ, Diatchenko L, Parisien M, Kang Y, Sarantopoulos KD, Martin ER, Smith SB, Maixner W, Levitt RC. Upadhyay U, et al. Among authors: kang y. PLoS Genet. 2019 Jun 14;15(6):e1008226. doi: 10.1371/journal.pgen.1008226. eCollection 2019 Jun. PLoS Genet. 2019. PMID: 31199789 Free PMC article.
Reversion mutation of cDNA CA8-204 minigene construct produces a truncated functional peptide that regulates calcium release in vitro and produces profound analgesia in vivo.
Upadhyay U, Zhuang GZ, Diatchenko L, Parisien M, Kang Y, Sarantopoulos KD, Martin ER, Smith SB, Maixner W, Levitt RC. Upadhyay U, et al. Among authors: kang y. Mamm Genome. 2020 Dec;31(9-12):287-294. doi: 10.1007/s00335-020-09848-y. Epub 2020 Nov 28. Mamm Genome. 2020. PMID: 33247772
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