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Identifying and quantifying proteolytic events and the natural N terminome by terminal amine isotopic labeling of substrates.
Kleifeld O, Doucet A, Prudova A, auf dem Keller U, Gioia M, Kizhakkedathu JN, Overall CM. Kleifeld O, et al. Nat Protoc. 2011 Sep 22;6(10):1578-611. doi: 10.1038/nprot.2011.382. Nat Protoc. 2011. PMID: 21959240
LysargiNase mirrors trypsin for protein C-terminal and methylation-site identification.
Huesgen PF, Lange PF, Rogers LD, Solis N, Eckhard U, Kleifeld O, Goulas T, Gomis-RĂ¼th FX, Overall CM. Huesgen PF, et al. Nat Methods. 2015 Jan;12(1):55-8. doi: 10.1038/nmeth.3177. Epub 2014 Nov 24. Nat Methods. 2015. PMID: 25419962
Isotopic labeling of terminal amines in complex samples identifies protein N-termini and protease cleavage products.
Kleifeld O, Doucet A, auf dem Keller U, Prudova A, Schilling O, Kainthan RK, Starr AE, Foster LJ, Kizhakkedathu JN, Overall CM. Kleifeld O, et al. Nat Biotechnol. 2010 Mar;28(3):281-8. doi: 10.1038/nbt.1611. Epub 2010 Mar 7. Nat Biotechnol. 2010. PMID: 20208520
Towards third generation matrix metalloproteinase inhibitors for cancer therapy.
Overall CM, Kleifeld O. Overall CM, et al. Br J Cancer. 2006 Apr 10;94(7):941-6. doi: 10.1038/sj.bjc.6603043. Br J Cancer. 2006. PMID: 16538215 Free PMC article. Review.
Oxidation of an exposed methionine instigates the aggregation of glyceraldehyde-3-phosphate dehydrogenase.
Samson AL, Knaupp AS, Kass I, Kleifeld O, Marijanovic EM, Hughes VA, Lupton CJ, Buckle AM, Bottomley SP, Medcalf RL. Samson AL, et al. J Biol Chem. 2014 Sep 26;289(39):26922-36. doi: 10.1074/jbc.M114.570275. Epub 2014 Aug 1. J Biol Chem. 2014. PMID: 25086035 Free PMC article.
Changes in protein abundance are observed in bacterial isolates from a natural host.
Rees MA, Stinear TP, Goode RJ, Coppel RL, Smith AI, Kleifeld O. Rees MA, et al. Front Cell Infect Microbiol. 2015 Oct 14;5:71. doi: 10.3389/fcimb.2015.00071. eCollection 2015. Front Cell Infect Microbiol. 2015. PMID: 26528441 Free PMC article.
Structural basis for potent slow binding inhibition of human matrix metalloproteinase-2 (MMP-2).
Rosenblum G, Meroueh SO, Kleifeld O, Brown S, Singson SP, Fridman R, Mobashery S, Sagi I. Rosenblum G, et al. J Biol Chem. 2003 Jul 18;278(29):27009-15. doi: 10.1074/jbc.M301139200. Epub 2003 Apr 4. J Biol Chem. 2003. PMID: 12679334
Active site electronic structure and dynamics during metalloenzyme catalysis.
Kleifeld O, Frenkel A, Martin JM, Sagi I. Kleifeld O, et al. Nat Struct Biol. 2003 Feb;10(2):98-103. doi: 10.1038/nsb889. Nat Struct Biol. 2003. PMID: 12524531
Structure of ubiquitylated-Rpn10 provides insight into its autoregulation mechanism.
Keren-Kaplan T, Zeev Peters L, Levin-Kravets O, Attali I, Kleifeld O, Shohat N, Artzi S, Zucker O, Pilzer I, Reis N, Glickman MH, Ben-Aroya S, Prag G. Keren-Kaplan T, et al. Nat Commun. 2016 Oct 4;7:12960. doi: 10.1038/ncomms12960. Nat Commun. 2016. PMID: 27698474 Free PMC article.
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