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Strategies for the identification of kinase substrates using analog-sensitive kinases.
Koch A, Hauf S. Koch A, et al. Eur J Cell Biol. 2010 Feb-Mar;89(2-3):184-93. doi: 10.1016/j.ejcb.2009.11.024. Epub 2010 Jan 12. Eur J Cell Biol. 2010. PMID: 20061049 Review.
Phosphorylation of proteins is a prevalent post-translational modification, which affects intracellular signaling in many ways. ...Despite technological advances that have made it possible to identify thousands of phosphorylation sites simultaneously, identification of the …
Phosphorylation of proteins is a prevalent post-translational modification, which affects intracellular signaling in many ways. ...De …
Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A, Krug K, Graf S, Koch A, Popic S, Hauf S, Macek B. Carpy A, et al. Among authors: koch a. Mol Cell Proteomics. 2014 Aug;13(8):1925-36. doi: 10.1074/mcp.M113.035824. Epub 2014 Apr 23. Mol Cell Proteomics. 2014. PMID: 24763107 Free PMC article.
To quantify cell cycle-dependent fluctuations on a proteome-wide scale, we performed integrative analysis of the proteome and phosphoproteome during the four major phases of the cell cycle in Schizosaccharomyces pombe. ...
To quantify cell cycle-dependent fluctuations on a proteome-wide scale, we performed integrative analysis of the proteome and phospho …
A chemical genetic approach for covalent inhibition of analogue-sensitive aurora kinase.
Koch A, Rode HB, Richters A, Rauh D, Hauf S. Koch A, et al. ACS Chem Biol. 2012 Apr 20;7(4):723-31. doi: 10.1021/cb200465c. Epub 2012 Feb 1. ACS Chem Biol. 2012. PMID: 22264160
The perturbation of protein kinases with small organic molecules is a powerful approach to dissect kinase function in complex biological systems. ...
The perturbation of protein kinases with small organic molecules is a powerful approach to dissect kinase function in complex biologi …
Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A, Krug K, Pengelley S, Macek B, Hauf S. Koch A, et al. Sci Signal. 2011 Jun 28;4(179):rs6. doi: 10.1126/scisignal.2001588. Sci Signal. 2011. PMID: 21712547
We applied site-specific quantitative phosphoproteomics in conjunction with chemical inhibition of Aurora to identify mitotic Aurora substrates in fission yeast on a proteome-wide scale. We detected 8000 phosphorylation events, of which we assigned almost 6000 to a
We applied site-specific quantitative phosphoproteomics in conjunction with chemical inhibition of Aurora to identify mitotic Aurora substra …
Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-Based Quantitative Proteomics and Phosphoproteomics in Fission Yeast.
Carpy A, Koch A, Bicho CC, Borek WE, Hauf S, Sawin KE, Maček B. Carpy A, et al. Among authors: koch a. Cold Spring Harb Protoc. 2017 Jun 1;2017(6):pdb.prot091686. doi: 10.1101/pdb.prot091686. Cold Spring Harb Protoc. 2017. PMID: 28572185
Modern mass spectrometry (MS)-based approaches are capable of identifying and quantifying thousands of proteins and phosphorylation events in a single biological experiment. Here we present a (phospho)proteomic workflow based on in-solution proteome digestion of sam …
Modern mass spectrometry (MS)-based approaches are capable of identifying and quantifying thousands of proteins and phosphorylation events i …
Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Technology in Fission Yeast.
Maček B, Carpy A, Koch A, Bicho CC, Borek WE, Hauf S, Sawin KE. Maček B, et al. Among authors: koch a. Cold Spring Harb Protoc. 2017 Jun 1;2017(6):pdb.top079814. doi: 10.1101/pdb.top079814. Cold Spring Harb Protoc. 2017. PMID: 28572211
Shotgun proteomics combined with stable isotope labeling by amino acids in cell culture (SILAC) is a powerful approach to quantify proteins and posttranslational modifications across the entire proteome. ...Both strategies have been used successfully in large-scale (phosph …
Shotgun proteomics combined with stable isotope labeling by amino acids in cell culture (SILAC) is a powerful approach to quantify pr …
Construction, Growth, and Harvesting of Fission Yeast Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Strains.
Koch A, Bicho CC, Borek WE, Carpy A, Maček B, Hauf S, Sawin KE. Koch A, et al. Cold Spring Harb Protoc. 2017 Jun 1;2017(6):pdb.prot091678. doi: 10.1101/pdb.prot091678. Cold Spring Harb Protoc. 2017. PMID: 28572184
Here we describe methods for the application of SILAC to fission yeast Schizosaccharomyces pombe using either labeled lysine or a combination of labeled lysine and labeled arginine. The latter approach is more complicated than the use of labeled lysine alone but may yield …
Here we describe methods for the application of SILAC to fission yeast Schizosaccharomyces pombe using either labeled lysine or a com …
A Yorkshire swine (Sus scrofa domesticus) model for nerve regeneration and ischemia based on the sciatic nerve and femoral artery.
Kinsley SE, Fernicola SD, Dingle ME, Williams MS, Richardson JM, Taylor D, de Vasconcellos JF, Malone TR, Blattner MR, Smith JK, Oliver A, Koch AL, Riddle LE, Reiter C, Culp WE, Caterson EJ, Nesti LJ, Talbot SG. Kinsley SE, et al. Among authors: koch al. Ann Anat. 2020 Sep 27:151587. doi: 10.1016/j.aanat.2020.151587. Online ahead of print. Ann Anat. 2020. PMID: 32998029 Spanish. No abstract available.
Good outcomes of modified Grammont and Langenskiöld technique in children with habitual patellar dislocation.
Musielak BJ, Premakumaran P, Janusz P, Dziurda M, Koch A, Walczak M. Musielak BJ, et al. Among authors: koch a. Knee Surg Sports Traumatol Arthrosc. 2020 Sep 26. doi: 10.1007/s00167-020-06284-y. Online ahead of print. Knee Surg Sports Traumatol Arthrosc. 2020. PMID: 32980886
Exposure to welding fumes suppresses the activity of T-helper cells.
Knobloch J, Casjens S, Lehnert M, Yanik SD, Körber S, Lotz A, Rupp J, Raulf M, Zschiesche W, Weiss T, Kronsbein J, Koch A, Brüning T, Pesch B. Knobloch J, et al. Among authors: koch a. Environ Res. 2020 Oct;189:109913. doi: 10.1016/j.envres.2020.109913. Epub 2020 Jul 12. Environ Res. 2020. PMID: 32980007
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