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Development of a homologous transformation system for the human pathogenic fungus Aspergillus fumigatus based on the pyrG gene encoding orotidine 5'-monophosphate decarboxylase.
Weidner G, d'Enfert C, Koch A, Mol PC, Brakhage AA. Weidner G, et al. Curr Genet. 1998 May;33(5):378-85. doi: 10.1007/s002940050350. Curr Genet. 1998. PMID: 9618589
DNA sequencing of the A. fumigatus pyrG1 mutant allele revealed that it encodes a truncated, non-functional, PyrG protein. Transformation of an A. fumigatus pyrG1 mutant with a plasmid carrying the novel pyrG2 allele constructed by in vitro mutagenesis …
DNA sequencing of the A. fumigatus pyrG1 mutant allele revealed that it encodes a truncated, non-functional, PyrG protein. Tra …
Isolation and characterization of a pigmentless-conidium mutant of Aspergillus fumigatus with altered conidial surface and reduced virulence.
Jahn B, Koch A, Schmidt A, Wanner G, Gehringer H, Bhakdi S, Brakhage AA. Jahn B, et al. Infect Immun. 1997 Dec;65(12):5110-7. doi: 10.1128/IAI.65.12.5110-5117.1997. Infect Immun. 1997. PMID: 9393803 Free PMC article.
This finding strongly suggests that the A. fumigatus mutant described here carries a single mutation which caused all of the observed phenotypes. Our results suggest that the conidium pigment or a structural feature related to it contributes to fungal resista …
This finding strongly suggests that the A. fumigatus mutant described here carries a single mutation which caused all of the o …
Cloning, sequencing, and heterologous expression of a cellulase-encoding cDNA (cbh1) from Penicillium janthinellum.
Koch A, Weigel CT, Schulz G. Koch A, et al. Gene. 1993 Feb 14;124(1):57-65. doi: 10.1016/0378-1119(93)90761-q. Gene. 1993. PMID: 8440481
From a Penicillium janthinellum cDNA library, two clones with 1.8- and 1.9-kb inserts were isolated by hybridization to a Trichoderma reesei cellulase-encoding gene probe (egl1). ...Expression of the 1.9-kb cDNA in the Escherichia coli T7 system led to the detection …
From a Penicillium janthinellum cDNA library, two clones with 1.8- and 1.9-kb inserts were isolated by hybridization to a Tric …
Endoglucanase II (EGII) of Penicillium janthinellum: cDNA sequence, heterologous expression and promotor analysis.
Mernitz G, Koch A, Henrissat B, Schulz G. Mernitz G, et al. Curr Genet. 1996 Apr;29(5):490-5. doi: 10.1007/BF02221519. Curr Genet. 1996. PMID: 8625430
The cellulose-binding domain of EGII represents a typical member of the A family of cellulases. The egl2 gene is only induced by cellulose or cellobiose and not by sophorose. A promotor fragment including 1 kb was cloned and sequenced. Three major transcripti …
The cellulose-binding domain of EGII represents a typical member of the A family of cellulases. The egl2 gene is only induced …
Plastidic (Pho1-type) phosphorylase isoforms in potato (Solanum tuberosum L.) plants: expression analysis and immunochemical characterization.
Albrecht T, Koch A, Lode A, Greve B, Schneider-Mergener J, Steup M. Albrecht T, et al. Planta. 2001 Aug;213(4):602-13. doi: 10.1007/s004250100525. Planta. 2001. PMID: 11556793
., two highly homologous Pho1-type sequences (designated as Pho1a and Pho1b, respectively) have been described that occur both in a homodimeric, (Pho1a)2, and a heterodimeric, Pho1a-Pho1b, state [U. ...We present a spatial and temporal analysis of the express …
., two highly homologous Pho1-type sequences (designated as Pho1a and Pho1b, respectively) have been described that occur both in a h …
Isolation and characterization of maltokinase (ATP:maltose 1-phosphotransferase) from Actinoplanes missouriensis.
Niehues B, Jossek R, Kramer U, Koch A, Jarling M, Schröder W, Pape H. Niehues B, et al. Arch Microbiol. 2003 Oct;180(4):233-9. doi: 10.1007/s00203-003-0575-y. Epub 2003 Jul 18. Arch Microbiol. 2003. PMID: 12879214
The enzyme of A. missouriensis responsible for this reaction was purified and characterized. This protein has an estimated molecular mass of 57 kDa and it is most likely a monomer. ...
The enzyme of A. missouriensis responsible for this reaction was purified and characterized. This protein has an estimated molecular …
[Value of polymerase chain reaction (PCR) in diagnosis of tuberculosis and mycobacterium infections caused by ubiquitous mycobacteria].
Rosenkranz S, Wassermann K, Seelig R, Klein R, Koch A, Beuckelmann DJ, Erdmann E. Rosenkranz S, et al. Pneumologie. 1997 Feb;51(2):78-86. Pneumologie. 1997. PMID: 9157455 German.
For ubiquitous mycobacteria, sensitivity of PCR was 60.0% relative to cultural identification and 62.5% relative to a proven disease. PCR is a useful method in the rapid diagnosis of pulmonary tuberculosis with excellent specificity but lack of sensitivity....
For ubiquitous mycobacteria, sensitivity of PCR was 60.0% relative to cultural identification and 62.5% relative to a proven disease. …
Dopamine/serotonin receptor ligands. Part 15: Oxygenation of the benz-indolo-azecine LE 300 leads to novel subnanomolar dopamine D1/D5 antagonists.
Enzensperger C, Kilian S, Ackermann M, Koch A, Kelch K, Lehmann J. Enzensperger C, et al. Bioorg Med Chem Lett. 2007 Mar 1;17(5):1399-402. doi: 10.1016/j.bmcl.2006.11.093. Epub 2006 Dec 3. Bioorg Med Chem Lett. 2007. PMID: 17188870
Studies of the toxic effects of norepinephrine on isolated cardiomyocytes of guinea-pigs.
Rump AF, Schierholz J, Biederbick W, Koch AE, Klaus W. Rump AF, et al. Arzneimittelforschung. 2001 Nov;51(11):873-6. doi: 10.1055/s-0031-1300130. Arzneimittelforschung. 2001. PMID: 11765587
Cell viability was assessed by morphologic examination (% of striated, rod-shaped cells), before and after a treatment period of 15 and 60 min by the measurement of intracellular enzyme activities in the supernatant of the suspension (lactate dehydrogenase, creatine kinase …
Cell viability was assessed by morphologic examination (% of striated, rod-shaped cells), before and after a treatment period of 15 a …
[Randomization in clinical studies. Empirically founded or only a dogma?].
Abel U, Koch A. Abel U, et al. Internist (Berl). 1997 Apr;38(4):318-24. doi: 10.1007/s001080050043. Internist (Berl). 1997. PMID: 9213570 Review. German. No abstract available.
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