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Bulky high-mannose-type N-glycan blocks the taste-modifying activity of miraculin.
Ito K, Sugawara T, Koizumi A, Nakajima K, Shimizu-Ibuka A, Shiroishi M, Asada H, Yurugi-Kobayashi T, Shimamura T, Asakura T, Masuda K, Ishiguro M, Misaka T, Iwata S, Kobayashi T, Abe K. Ito K, et al. Among authors: koizumi a. Biochim Biophys Acta. 2010 Sep;1800(9):986-92. doi: 10.1016/j.bbagen.2010.06.003. Epub 2010 Jun 11. Biochim Biophys Acta. 2010. PMID: 20542090 Free article.
Human sweet taste receptor mediates acid-induced sweetness of miraculin.
Koizumi A, Tsuchiya A, Nakajima K, Ito K, Terada T, Shimizu-Ibuka A, Briand L, Asakura T, Misaka T, Abe K. Koizumi A, et al. Proc Natl Acad Sci U S A. 2011 Oct 4;108(40):16819-24. doi: 10.1073/pnas.1016644108. Epub 2011 Sep 26. Proc Natl Acad Sci U S A. 2011. PMID: 21949380 Free PMC article.
Cysteine-to-serine shuffling using a Saccharomyces cerevisiae expression system improves protein secretion: case of a nonglycosylated mutant of miraculin, a taste-modifying protein.
Ito K, Sugawara T, Koizumi A, Nakajima K, Shimizu-Ibuka A, Shiroishi M, Asada H, Yurugi-Kobayashi T, Shimamura T, Asakura T, Misaka T, Iwata S, Kobayashi T, Abe K. Ito K, et al. Among authors: koizumi a. Biotechnol Lett. 2011 Jan;33(1):103-7. doi: 10.1007/s10529-010-0399-1. Epub 2010 Oct 9. Biotechnol Lett. 2011. PMID: 20936326 Free article.
Microbial production of sensory-active miraculin.
Ito K, Asakura T, Morita Y, Nakajima K, Koizumi A, Shimizu-Ibuka A, Masuda K, Ishiguro M, Terada T, Maruyama J, Kitamoto K, Misaka T, Abe K. Ito K, et al. Among authors: koizumi a. Biochem Biophys Res Commun. 2007 Aug 24;360(2):407-11. doi: 10.1016/j.bbrc.2007.06.064. Epub 2007 Jun 19. Biochem Biophys Res Commun. 2007. PMID: 17592723
829 results