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A thin slice preparation for patch clamp recordings from neurones of the mammalian central nervous system.
Edwards FA, Konnerth A, Sakmann B, Takahashi T. Edwards FA, et al. Pflugers Arch. 1989 Sep;414(5):600-12. doi: 10.1007/BF00580998. Pflugers Arch. 1989. PMID: 2780225
(1) A preparation is described which allows patch clamp recordings to be made on mammalian central nervous system (CNS) neurones in situ. (2) A vibrating tissue slicer was used to cut thin slices in which individual neurones could be identified visually. ...These te …
(1) A preparation is described which allows patch clamp recordings to be made on mammalian central nervous system (CNS) neurones in s …
Two-photon chloride imaging in neurons of brain slices.
Marandi N, Konnerth A, Garaschuk O. Marandi N, et al. Pflugers Arch. 2002 Dec;445(3):357-65. doi: 10.1007/s00424-002-0933-7. Epub 2002 Sep 20. Pflugers Arch. 2002. PMID: 12466938
Brief incubation with MQAE produced a robust loading of cells in slices from various brain regions including hippocampus, cortex and cerebellum. ...Thus, MQAE-based two-photon microscopy promises to be a valuable technique for many applications requiring chloride im …
Brief incubation with MQAE produced a robust loading of cells in slices from various brain regions including hippocampus, cortex and …
Optical monitoring of brain function in vivo: from neurons to networks.
Garaschuk O, Milos RI, Grienberger C, Marandi N, Adelsberger H, Konnerth A. Garaschuk O, et al. Pflugers Arch. 2006 Dec;453(3):385-96. doi: 10.1007/s00424-006-0150-x. Epub 2006 Oct 18. Pflugers Arch. 2006. PMID: 17047983 Review.
A promising approach for such analyses is two-photon microscopy in combination with appropriate cell labeling techniques. Here, we review the multi-cell bolus loading (MCBL) method that involves the use of membrane-permeant fluorescent indicator dyes. ...
A promising approach for such analyses is two-photon microscopy in combination with appropriate cell labeling techniques. Here, we re
Quantitative single-cell RT-PCR and Ca2+ imaging in brain slices.
Durand GM, Marandi N, Herberger SD, Blum R, Konnerth A. Durand GM, et al. Pflugers Arch. 2006 Mar;451(6):716-26. doi: 10.1007/s00424-005-1514-3. Epub 2005 Oct 7. Pflugers Arch. 2006. PMID: 16211366
We have established a quantitative reverse transcriptase-PCR (RT-PCR) approach for the analysis of RNA transcript levels in individual cells of living brain slices. ...Our study revealed a developmental switch from an average of 15 NR2B copies/cell at postnatal day …
We have established a quantitative reverse transcriptase-PCR (RT-PCR) approach for the analysis of RNA transcript levels in individua …
In vivo deep two-photon imaging of neural circuits with the fluorescent Ca2+ indicator Cal-590.
Tischbirek CH, Birkner A, Konnerth A. Tischbirek CH, et al. J Physiol. 2017 May 15;595(10):3097-3105. doi: 10.1113/JP272790. Epub 2017 Feb 5. J Physiol. 2017. PMID: 27995645 Free PMC article. Review.
Specific features of Cal-590-based in vivo Ca(2+) two-photon imaging include a good signal-to-noise ratio, fast kinetics and a linear dependence of the Ca(2+) transients on the number of action potentials. ...Overall, Cal-590-based two-photon microscopy emerges as …
Specific features of Cal-590-based in vivo Ca(2+) two-photon imaging include a good signal-to-noise ratio, fast kinetics and a
"In vivo" monitoring of neuronal network activity in zebrafish by two-photon Ca(2+) imaging.
Brustein E, Marandi N, Kovalchuk Y, Drapeau P, Konnerth A. Brustein E, et al. Pflugers Arch. 2003 Sep;446(6):766-73. doi: 10.1007/s00424-003-1138-4. Epub 2003 Jul 22. Pflugers Arch. 2003. PMID: 12883893
The zebrafish larva is a powerful model for the analysis of behaviour and the underlying neuronal network activity during early stages of development. ...Using two-photon imaging, we could monitor Ca(2+) signals simultaneously from a large population of spinal neuro …
The zebrafish larva is a powerful model for the analysis of behaviour and the underlying neuronal network activity during early stage …
Two-photon Na+ imaging in spines and fine dendrites of central neurons.
Rose CR, Kovalchuk Y, Eilers J, Konnerth A. Rose CR, et al. Pflugers Arch. 1999 Dec;439(1-2):201-7. doi: 10.1007/s004249900123. Pflugers Arch. 1999. PMID: 10651018
The action-potential-induced [Na+]i transients reached values of 4 mM for a train of 20 APs and monotonically decayed with a time constant of several seconds. ...Our results demonstrate that two-photon Na+ imaging represents a powerful tool for extending our …
The action-potential-induced [Na+]i transients reached values of 4 mM for a train of 20 APs and monotonically decayed with a t …
Cell-type specific expression of ATP-sensitive potassium channels in the rat hippocampus.
Zawar C, Plant TD, Schirra C, Konnerth A, Neumcke B. Zawar C, et al. J Physiol. 1999 Jan 15;514 ( Pt 2)(Pt 2):327-41. doi: 10.1111/j.1469-7793.1999.315ae.x. J Physiol. 1999. PMID: 9852317 Free PMC article.
The patch-clamp technique was used to study the sensitivity of whole-cell currents to diazoxide (0.3 mM), a KATP channel opener, and to tolbutamide (0.5 mM) or glibenclamide (20 microM), two KATP channel inhibitors. ...The reversal potential of the diazoxide-induced curren …
The patch-clamp technique was used to study the sensitivity of whole-cell currents to diazoxide (0.3 mM), a KATP channel opener, and …
Developmental profile and synaptic origin of early network oscillations in the CA1 region of rat neonatal hippocampus.
Garaschuk O, Hanse E, Konnerth A. Garaschuk O, et al. J Physiol. 1998 Feb 15;507 ( Pt 1)(Pt 1):219-36. doi: 10.1111/j.1469-7793.1998.219bu.x. J Physiol. 1998. PMID: 9490842 Free PMC article.
By applying fura-2-based fluorometric calcium imaging to neonatal rat hippocampal slices we identified a developmentally regulated spontaneous neuronal activity in the CA1 region of the hippocampus. ...The ENOs were blocked by tetrodotoxin (TTX) and by a reduction i …
By applying fura-2-based fluorometric calcium imaging to neonatal rat hippocampal slices we identified a developmentally regulated sp …
Molecular determinants of NMDA receptor function in GABAergic neurones of rat forebrain.
Plant T, Schirra C, Garaschuk O, Rossier J, Konnerth A. Plant T, et al. J Physiol. 1997 Feb 15;499 ( Pt 1)(Pt 1):47-63. doi: 10.1113/jphysiol.1997.sp021910. J Physiol. 1997. PMID: 9061639 Free PMC article.
A variety of NR2 subunit combinations were detected in the same population of neurones. When tested for NR2A-C, all but one neurone were shown to express mRNA for NR2B. ...Currents activated by NMDA had a high sensitivity to extracellular Mg2+. 5. The fraction of th
A variety of NR2 subunit combinations were detected in the same population of neurones. When tested for NR2A-C, all but one neurone w
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