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The construction and application of diploid sake yeast with a homozygous mutation in the FAS2 gene.
Kotaka A, Sahara H, Hata Y. Kotaka A, et al. J Biosci Bioeng. 2010 Dec;110(6):675-8. doi: 10.1016/j.jbiosc.2010.07.007. Epub 2010 Aug 12. J Biosci Bioeng. 2010. PMID: 20708434
Isoflavone aglycones production from isoflavone glycosides by display of beta-glucosidase from Aspergillus oryzae on yeast cell surface.
Kaya M, Ito J, Kotaka A, Matsumura K, Bando H, Sahara H, Ogino C, Shibasaki S, Kuroda K, Ueda M, Kondo A, Hata Y. Kaya M, et al. Among authors: kotaka a. Appl Microbiol Biotechnol. 2008 May;79(1):51-60. doi: 10.1007/s00253-008-1393-6. Epub 2008 Mar 14. Appl Microbiol Biotechnol. 2008. PMID: 18340445
Three enzymes were independently displayed on the cell surface of a yeast Saccharomyces cerevisiae as a fusion protein with alpha-agglutinin. ...
Three enzymes were independently displayed on the cell surface of a yeast Saccharomyces cerevisiae as a fusion protein with al …
Efficient and direct fermentation of starch to ethanol by sake yeast strains displaying fungal glucoamylases.
Kotaka A, Sahara H, Hata Y, Abe Y, Kondo A, Kato-Murai M, Kuroda K, Ueda M. Kotaka A, et al. Biosci Biotechnol Biochem. 2008 May;72(5):1376-9. doi: 10.1271/bbb.70825. Epub 2008 May 7. Biosci Biotechnol Biochem. 2008. PMID: 18460787
Direct ethanol production from barley beta-glucan by sake yeast displaying Aspergillus oryzae beta-glucosidase and endoglucanase.
Kotaka A, Bando H, Kaya M, Kato-Murai M, Kuroda K, Sahara H, Hata Y, Kondo A, Ueda M. Kotaka A, et al. J Biosci Bioeng. 2008 Jun;105(6):622-7. doi: 10.1263/jbb.105.622. J Biosci Bioeng. 2008. PMID: 18640601
When direct ethanol fermentation from 20 g/l barley beta-glucan as a model substrate was performed with the codisplaying strain, the ethanol concentration reached 7.94 g/l after 24 h of fermentation. ...These results showed that sake yeast displaying A. oryzae cellu …
When direct ethanol fermentation from 20 g/l barley beta-glucan as a model substrate was performed with the codisplaying strain, the …
Enhancement of display efficiency in yeast display system by vector engineering and gene disruption.
Kuroda K, Matsui K, Higuchi S, Kotaka A, Sahara H, Hata Y, Ueda M. Kuroda K, et al. Among authors: kotaka a. Appl Microbiol Biotechnol. 2009 Mar;82(4):713-9. doi: 10.1007/s00253-008-1808-4. Epub 2009 Jan 3. Appl Microbiol Biotechnol. 2009. PMID: 19123001
To evaluate the display efficiency by flow cytometric analysis, DsRed-monomer fused with FLAG-tag was displayed and immunostained as a model protein. ...The improved yeast display system would be useful in a wider range of its applications in biotechnology....
To evaluate the display efficiency by flow cytometric analysis, DsRed-monomer fused with FLAG-tag was displayed and immunostained as a
Efficient generation of recessive traits in diploid sake yeast by targeted gene disruption and loss of heterozygosity.
Kotaka A, Sahara H, Kondo A, Ueda M, Hata Y. Kotaka A, et al. Appl Microbiol Biotechnol. 2009 Feb;82(2):387-95. doi: 10.1007/s00253-008-1833-3. Epub 2009 Jan 10. Appl Microbiol Biotechnol. 2009. PMID: 19137286
Until now, only a few recessive mutants of sake yeast have been obtained. To solve this problem, we developed the high-efficiency loss of heterozygosity (HELOH) method, which applies a two-step gene disruption. ...Subsequently, spontaneous loss of heterozygosity (LO …
Until now, only a few recessive mutants of sake yeast have been obtained. To solve this problem, we developed the high-efficiency los …
Using promoter replacement and selection for loss of heterozygosity to generate an industrially applicable sake yeast strain that homozygously overproduces isoamyl acetate.
Sahara H, Kotaka A, Kondo A, Ueda M, Hata Y. Sahara H, et al. Among authors: kotaka a. J Biosci Bioeng. 2009 Nov;108(5):359-64. doi: 10.1016/j.jbiosc.2009.05.002. J Biosci Bioeng. 2009. PMID: 19804856
Enhancement of beta-glucosidase activity on the cell-surface of sake yeast by disruption of SED1.
Kotaka A, Sahara H, Kuroda K, Kondo A, Ueda M, Hata Y. Kotaka A, et al. J Biosci Bioeng. 2010 May;109(5):442-6. doi: 10.1016/j.jbiosc.2009.11.003. Epub 2009 Dec 4. J Biosci Bioeng. 2010. PMID: 20347765
Enhancement of malate-production and increase in sensitivity to dimethyl succinate by mutation of the VID24 gene in Saccharomyces cerevisiae.
Negoro H, Kotaka A, Matsumura K, Tsutsumi H, Hata Y. Negoro H, et al. Among authors: kotaka a. J Biosci Bioeng. 2016 Jun;121(6):665-671. doi: 10.1016/j.jbiosc.2015.11.012. Epub 2016 Mar 13. J Biosci Bioeng. 2016. PMID: 26983942
Mutation in the peroxin-coding gene PEX22 contributing to high malate production in Saccharomyces cerevisiae.
Negoro H, Sakamoto M, Kotaka A, Matsumura K, Hata Y. Negoro H, et al. Among authors: kotaka a. J Biosci Bioeng. 2018 Feb;125(2):211-217. doi: 10.1016/j.jbiosc.2017.08.010. Epub 2017 Sep 14. J Biosci Bioeng. 2018. PMID: 28919252
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