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Long-term proliferating early pre B cell lines and clones with the potential to develop to surface Ig-positive, mitogen reactive B cells in vitro and in vivo.
Rolink A, Kudo A, Karasuyama H, Kikuchi Y, Melchers F. Rolink A, et al. EMBO J. 1991 Feb;10(2):327-36. EMBO J. 1991. PMID: 1991449 Free PMC article.
Cell lines and clones were established from PB76-positive mouse fetal liver at day 13 and 14 of gestation, which proliferated with division times of a day in serum-substituted cultures under the stimulatory influence of adherent stromal cells and the cytokine IL-7 for peri …
Cell lines and clones were established from PB76-positive mouse fetal liver at day 13 and 14 of gestation, which proliferated with division …
Conversion of normal Ly-1-positive B-lineage cells into Ly-1-positive macrophages in long-term bone marrow cultures.
Katoh S, Tominaga A, Migita M, Kudo A, Takatsu K. Katoh S, et al. Dev Immunol. 1990;1(2):113-25. doi: 10.1155/1990/28760. Dev Immunol. 1990. PMID: 2136207 Free PMC article.
We obtained eight different cell lines in the long-term bone marrow culture system that showed a germ-line configuration of the joining (J) region segments of the Ig heavy-chain (IgH) genes. ...After other early lymphoid lines (J1, J8, and J13) were maintained by co-cultur …
We obtained eight different cell lines in the long-term bone marrow culture system that showed a germ-line configuration of the joini …
The expression of the mouse VpreB/lambda 5 locus in transformed cell lines and tumors of the B lineage differentiation pathway.
Kudo A, Thalmann P, Sakaguchi N, Davidson WF, Pierce JH, Kearney JF, Reth M, Rolink A, Melchers F. Kudo A, et al. Int Immunol. 1992 Aug;4(8):831-40. doi: 10.1093/intimm/4.8.831. Int Immunol. 1992. PMID: 1419955
The expression of RNA transcripts from two pre B lymphocyte related genes, VpreB and lambda 5, has been studied in a series of transformed cell lines which appear frozen at different states of B lineage differentiation, from early progenitors to surface Ig positive B cells …
The expression of RNA transcripts from two pre B lymphocyte related genes, VpreB and lambda 5, has been studied in a series of transf …
Organization of the murine Ig-related lambda 5 gene transcribed selectively in pre-B lymphocytes.
Kudo A, Sakaguchi N, Melchers F. Kudo A, et al. EMBO J. 1987 Jan;6(1):103-7. EMBO J. 1987. PMID: 3107979 Free PMC article.
Comparison of the nucleotide sequence of a 1 kb cDNA clone with the sequence of a genomic clone isolated from 70Z/3 murine pre-B lymphoma cells shows lambda 5 is composed of three exons spanning a 3.75 kb DNA segment. ...Exon I contains a cap-site and …
Comparison of the nucleotide sequence of a 1 kb cDNA clone with the sequence of a genomic clone isolated from 70Z/3 murine pre …
A second gene, VpreB in the lambda 5 locus of the mouse, which appears to be selectively expressed in pre-B lymphocytes.
Kudo A, Melchers F. Kudo A, et al. EMBO J. 1987 Aug;6(8):2267-72. EMBO J. 1987. PMID: 3117530 Free PMC article.
We have now found, 4.6 kb upstream of lambda 5, another gene composed of two exons which is selectively expressed in pre-B cell lines as a 0.85 kb mRNA potentially coding for a protein of 142 amino acids including a 19 amino acid-long signal peptide. ...The p …
We have now found, 4.6 kb upstream of lambda 5, another gene composed of two exons which is selectively expressed in pre-B cell lines as …
The surrogate light chain in B-cell development.
Melchers F, Karasuyama H, Haasner D, Bauer S, Kudo A, Sakaguchi N, Jameson B, Rolink A. Melchers F, et al. Immunol Today. 1993 Feb;14(2):60-8. doi: 10.1016/0167-5699(93)90060-X. Immunol Today. 1993. PMID: 8166770 Review.
The proteins encoded by the VpreB and lambda 5 genes associate with each other to form a light (L) chain-like structure, the surrogate L chain. ...
The proteins encoded by the VpreB and lambda 5 genes associate with each other to form a light (L) chain-like structure, the surrogat …
Two pathways of B-lymphocyte development in mouse bone marrow and the roles of surrogate L chain in this development.
Rolink A, Karasuyama H, Haasner D, Grawunder U, Mårtensson IL, Kudo A, Melchers F. Rolink A, et al. Immunol Rev. 1994 Feb;137:185-201. doi: 10.1111/j.1600-065x.1994.tb00665.x. Immunol Rev. 1994. PMID: 8034335 Review. No abstract available.
B cell development in mice with a defective lambda 5 gene.
Rolink A, Karasuyama H, Grawunder U, Haasner D, Kudo A, Melchers F. Rolink A, et al. Eur J Immunol. 1993 Jun;23(6):1284-8. doi: 10.1002/eji.1830230614. Eur J Immunol. 1993. PMID: 7684685
The surrogate light chain encoded by the two pre-B cell-specific genes VpreB and lambda 5 plays a critical role in B cell development of the mouse. It has been shown that targeted disruption of the lambda 5 gene results in a depletion of B220+ CD43- IgM-pre-B cells …
The surrogate light chain encoded by the two pre-B cell-specific genes VpreB and lambda 5 plays a critical role in B cell development …
Structure and pre-B lymphocyte restricted expression of the VpreB in humans and conservation of its structure in other mammalian species.
Bauer SR, Kudo A, Melchers F. Bauer SR, et al. EMBO J. 1988 Jan;7(1):111-6. EMBO J. 1988. PMID: 3258819 Free PMC article.
Sequences encoding the entire putative second framework region and a stretch in the third framework region are identical in human and mouse VpreB. the human VpreB gene appears to be selectively expressed in human pre-B cell lines as an 0.85 kb poly(A)+ RNA. Its expr …
Sequences encoding the entire putative second framework region and a stretch in the third framework region are identical in human and …
Localization of the murine lambda 5 gene on chromosome 16.
Kudo A, Pravtcheva D, Sakaguchi N, Ruddle FH, Melchers F. Kudo A, et al. Genomics. 1987 Nov;1(3):277-9. doi: 10.1016/0888-7543(87)90055-3. Genomics. 1987. PMID: 3128472
The chromosomal location of the murine lambda 5 gene was analyzed by Southern hybridization using restriction enzyme-digested DNA from a panel of 15 mouse X hamster somatic cell hybrids. ...Southern hybridization with the murine lambda 1 gene as a probe detected the …
The chromosomal location of the murine lambda 5 gene was analyzed by Southern hybridization using restriction enzyme-digested DNA from a
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