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Recombinase-mediated cassette exchange (RMCE) - a rapidly-expanding toolbox for targeted genomic modifications.
Turan S, Zehe C, Kuehle J, Qiao J, Bode J. Turan S, et al. Among authors: kuehle j. Gene. 2013 Feb 15;515(1):1-27. doi: 10.1016/j.gene.2012.11.016. Epub 2012 Nov 29. Gene. 2013. PMID: 23201421 Review.
Protein transduction from retroviral Gag precursors.
Voelkel C, Galla M, Maetzig T, Warlich E, Kuehle J, Zychlinski D, Bode J, Cantz T, Schambach A, Baum C. Voelkel C, et al. Among authors: kuehle j. Proc Natl Acad Sci U S A. 2010 Apr 27;107(17):7805-10. doi: 10.1073/pnas.0914517107. Epub 2010 Apr 12. Proc Natl Acad Sci U S A. 2010. PMID: 20385817 Free PMC article.
Multiplexing RMCE: versatile extensions of the Flp-recombinase-mediated cassette-exchange technology.
Turan S, Kuehle J, Schambach A, Baum C, Bode J. Turan S, et al. Among authors: kuehle j. J Mol Biol. 2010 Sep 10;402(1):52-69. doi: 10.1016/j.jmb.2010.07.015. Epub 2010 Jul 19. J Mol Biol. 2010. PMID: 20650281
Lentiviral vector design and imaging approaches to visualize the early stages of cellular reprogramming.
Warlich E, Kuehle J, Cantz T, Brugman MH, Maetzig T, Galla M, Filipczyk AA, Halle S, Klump H, Schöler HR, Baum C, Schroeder T, Schambach A. Warlich E, et al. Among authors: kuehle j. Mol Ther. 2011 Apr;19(4):782-9. doi: 10.1038/mt.2010.314. Epub 2011 Feb 1. Mol Ther. 2011. PMID: 21285961 Free PMC article.
Avoiding cytotoxicity of transposases by dose-controlled mRNA delivery.
Galla M, Schambach A, Falk CS, Maetzig T, Kuehle J, Lange K, Zychlinski D, Heinz N, Brugman MH, Göhring G, Izsvák Z, Ivics Z, Baum C. Galla M, et al. Among authors: kuehle j. Nucleic Acids Res. 2011 Sep 1;39(16):7147-60. doi: 10.1093/nar/gkr384. Epub 2011 May 23. Nucleic Acids Res. 2011. PMID: 21609958 Free PMC article.
Modified lentiviral LTRs allow Flp recombinase-mediated cassette exchange and in vivo tracing of "factor-free" induced pluripotent stem cells.
Kuehle J, Turan S, Cantz T, Hoffmann D, Suerth JD, Maetzig T, Zychlinski D, Klein C, Steinemann D, Baum C, Bode J, Schambach A. Kuehle J, et al. Mol Ther. 2014 May;22(5):919-28. doi: 10.1038/mt.2014.4. Epub 2014 Jan 17. Mol Ther. 2014. PMID: 24434935 Free PMC article.
All-in-One inducible lentiviral vector systems based on drug controlled FLP recombinase.
Maetzig T, Kuehle J, Schwarzer A, Turan S, Rothe M, Chaturvedi A, Morgan M, Ha TC, Heuser M, Hammerschmidt W, Baum C, Schambach A. Maetzig T, et al. Among authors: kuehle j. Biomaterials. 2014 May;35(14):4345-56. doi: 10.1016/j.biomaterials.2014.01.057. Epub 2014 Feb 14. Biomaterials. 2014. PMID: 24529624
Non-integrating gamma-retroviral vectors as a versatile tool for transient zinc-finger nuclease delivery.
Bobis-Wozowicz S, Galla M, Alzubi J, Kuehle J, Baum C, Schambach A, Cathomen T. Bobis-Wozowicz S, et al. Among authors: kuehle j. Sci Rep. 2014 Apr 11;4:4656. doi: 10.1038/srep04656. Sci Rep. 2014. PMID: 24722320 Free PMC article.
Potent and reversible lentiviral vector restriction in murine induced pluripotent stem cells.
Geis FK, Galla M, Hoffmann D, Kuehle J, Zychlinski D, Maetzig T, Schott JW, Schwarzer A, Goffinet C, Goff SP, Schambach A. Geis FK, et al. Among authors: kuehle j. Retrovirology. 2017 May 31;14(1):34. doi: 10.1186/s12977-017-0358-1. Retrovirology. 2017. PMID: 28569216 Free PMC article.
Genetic Modification of T Cells with Chimeric Antigen Receptors: A Laboratory Manual.
Golumba-Nagy V, Kuehle J, Abken H. Golumba-Nagy V, et al. Among authors: kuehle j. Hum Gene Ther Methods. 2017 Dec;28(6):302-309. doi: 10.1089/hgtb.2017.083. Epub 2017 Jul 24. Hum Gene Ther Methods. 2017. PMID: 28741380
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