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A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation.
Choi KH, Kumar A, Schweizer HP. Choi KH, et al. J Microbiol Methods. 2006 Mar;64(3):391-7. doi: 10.1016/j.mimet.2005.06.001. Epub 2005 Jun 28. J Microbiol Methods. 2006. PMID: 15987659
A rapid microcentrifuge-based method is described for preparation of Pseudomonas aeruginosa electrocompetent cells with up to 10,000-fold increased transformation efficiencies over existing procedures. ...
A rapid microcentrifuge-based method is described for preparation of Pseudomonas aeruginosa electrocompetent cells with up to 10,000-
Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomallei.
Choi KH, Mima T, Casart Y, Rholl D, Kumar A, Beacham IR, Schweizer HP. Choi KH, et al. Appl Environ Microbiol. 2008 Feb;74(4):1064-75. doi: 10.1128/AEM.02430-07. Epub 2007 Dec 21. Appl Environ Microbiol. 2008. PMID: 18156318 Free PMC article.
Because of Burkholderia pseudomallei's classification as a select agent in the United States, genetic manipulation of this bacterium is strictly regulated. ...Lastly, the deletion mutation was complemented by a chromosomally integrated mini-Tn7 element carrying the …
Because of Burkholderia pseudomallei's classification as a select agent in the United States, genetic manipulation of this bacterium …
Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b.
Kumar A, Chua KL, Schweizer HP. Kumar A, et al. Antimicrob Agents Chemother. 2006 Oct;50(10):3460-3. doi: 10.1128/AAC.00440-06. Antimicrob Agents Chemother. 2006. PMID: 17005832 Free PMC article.
Construction and integration of recombinant mini-Tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and biosafe Pseudomonas aeruginosa host was validated as a generally applicable method for studies of uncharacterized bacterial efflux pump …
Construction and integration of recombinant mini-Tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and bi …
Expression of resistance-nodulation-cell-division efflux pumps in commonly used Burkholderia pseudomallei strains and clinical isolates from northern Australia.
Kumar A, Mayo M, Trunck LA, Cheng AC, Currie BJ, Schweizer HP. Kumar A, et al. Trans R Soc Trop Med Hyg. 2008 Dec;102 Suppl 1:S145-51. doi: 10.1016/S0035-9203(08)70032-4. Trans R Soc Trop Med Hyg. 2008. PMID: 19121677
Therapy for this disease is lengthy and limited to only a few antibiotics because of this bacterium's intrinsic antibiotic resistance to many clinically useful antibiotics. ...
Therapy for this disease is lengthy and limited to only a few antibiotics because of this bacterium's intrinsic antibiotic resistance …
Creation of a drug-sensitive reporter strain of Pseudomonas aeruginosa as a tool for the rapid screening of antimicrobial products.
Engelberg R, Danielson A, Wang S, Singh M, Wai A, Sorensen J, Duan K, Hausner G, Kumar A. Engelberg R, et al. J Microbiol Methods. 2018 Sep;152:1-6. doi: 10.1016/j.mimet.2018.07.001. Epub 2018 Jul 7. J Microbiol Methods. 2018. PMID: 30017996
Soil fungi are known to produce a variety of antimicrobials but rapid screening of fungi that produce such compounds remains a challenge. In this work, we used a hyper-susceptible strain of Pseudomonas aeruginosa to create a luminescent-reporter strain …
Soil fungi are known to produce a variety of antimicrobials but rapid screening of fungi that produce such compounds remains a
Mini-Tn7 vectors as genetic tools for single copy gene cloning in Acinetobacter baumannii.
Kumar A, Dalton C, Cortez-Cordova J, Schweizer HP. Kumar A, et al. J Microbiol Methods. 2010 Sep;82(3):296-300. doi: 10.1016/j.mimet.2010.07.002. Epub 2010 Jul 16. J Microbiol Methods. 2010. PMID: 20638421
In this study, we show the application of the mini-Tn7-based single copy insertion system in A. baumannii. Mini-Tn7 elements are known to integrate at a naturally evolved, therefore presumably neutral location (intergenic region) downstream of the glmS gene (glucosa …
In this study, we show the application of the mini-Tn7-based single copy insertion system in A. baumannii. Mini-Tn7 elements are know …
MexXY efflux pump overexpression and aminoglycoside resistance in cystic fibrosis isolates of Pseudomonas aeruginosa from chronic infections.
Singh M, Yau YCW, Wang S, Waters V, Kumar A. Singh M, et al. Can J Microbiol. 2017 Dec;63(12):929-938. doi: 10.1139/cjm-2017-0380. Epub 2017 Sep 18. Can J Microbiol. 2017. PMID: 28922614
Analysis of regulatory genes that control the expression of these 4 efflux pumps revealed a number of previously uncharacterized mutations. ...
Analysis of regulatory genes that control the expression of these 4 efflux pumps revealed a number of previously uncharacterized muta …
Bacterial resistance to antibiotics: active efflux and reduced uptake.
Kumar A, Schweizer HP. Kumar A, et al. Adv Drug Deliv Rev. 2005 Jul 29;57(10):1486-513. doi: 10.1016/j.addr.2005.04.004. Adv Drug Deliv Rev. 2005. PMID: 15939505 Review.
Antibiotic resistance of bacterial pathogens is a fast emerging global crisis and an understanding of the underlying resistance mechanisms is paramount for design and development of new therapeutic strategies. ...
Antibiotic resistance of bacterial pathogens is a fast emerging global crisis and an understanding of the underlying resistance mecha …
Evidence of MexT-independent overexpression of MexEF-OprN multidrug efflux pump of Pseudomonas aeruginosa in presence of metabolic stress.
Kumar A, Schweizer HP. Kumar A, et al. PLoS One. 2011;6(10):e26520. doi: 10.1371/journal.pone.0026520. Epub 2011 Oct 24. PLoS One. 2011. PMID: 22039504 Free PMC article.
Although the mexEF-oprN operon is uninducible in this mutant due to a 8-bp mexT insertion present in some P. aeruginosa PAO1 strains, the large colony variants expressed high levels of MexEF-OprN. ...A search for possible regulators of mexEF-oprN expression using tr …
Although the mexEF-oprN operon is uninducible in this mutant due to a 8-bp mexT insertion present in some P. aeruginosa PAO1 strains, …
Tn7-Based Single-Copy Insertion Vectors for Acinetobacter baumannii.
Ducas-Mowchun K, De Silva PM, Patidar R, Schweizer HP, Kumar A. Ducas-Mowchun K, et al. Methods Mol Biol. 2019;1946:135-150. doi: 10.1007/978-1-4939-9118-1_13. Methods Mol Biol. 2019. PMID: 30798551
Acinetobacter baumannii is considered a problematic Gram-negative pathogen due to its widespread resistance to antibiotics. Understanding of resistance mechanisms in A. baumannii is critical for designing new and effective therapeutic options. However, this is hampe …
Acinetobacter baumannii is considered a problematic Gram-negative pathogen due to its widespread resistance to antibiotics. Understan …
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