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Promoter selectivity control of Escherichia coli RNA polymerase by ionic strength: differential recognition of osmoregulated promoters by E sigma D and E sigma S holoenzymes.
Ding Q, Kusano S, Villarejo M, Ishihama A. Ding Q, et al. Mol Microbiol. 1995 May;16(4):649-56. doi: 10.1111/j.1365-2958.1995.tb02427.x. Mol Microbiol. 1995. PMID: 7476160
Under conditions of low ionic strength, the osmB and osmY promoters were transcribed by both E sigma D and E sigma S. Addition of up to 400 mM potassium glutamate (K glutamate), mimicking the intracellular ionic conditions under hyperosmotic stress, specifically enhanced t …
Under conditions of low ionic strength, the osmB and osmY promoters were transcribed by both E sigma D and E sigma S. Addition of up …
Functional interaction of Escherichia coli RNA polymerase with inorganic polyphosphate.
Kusano S, Ishihama A. Kusano S, et al. Genes Cells. 1997 Jul;2(7):433-41. doi: 10.1046/j.1365-2443.1997.13203301320330.x. Genes Cells. 1997. PMID: 9366549
BACKGROUND: RNA polymerase from the stationary growth phase cells of Escherichia coli is tightly associated with an acidic compound(s) and exhibits altered promoter selectivity, with reduced transcriptional activity of the genes highly expressed in the exponentially growin …
BACKGROUND: RNA polymerase from the stationary growth phase cells of Escherichia coli is tightly associated with an acidic compound(s
Effects of H-NS and potassium glutamate on sigmaS- and sigma70-directed transcription in vitro from osmotically regulated P1 and P2 promoters of proU in Escherichia coli.
Rajkumari K, Kusano S, Ishihama A, Mizuno T, Gowrishankar J. Rajkumari K, et al. J Bacteriol. 1996 Jul;178(14):4176-81. doi: 10.1128/jb.178.14.4176-4181.1996. J Bacteriol. 1996. PMID: 8763946 Free PMC article.
We have used supercoiled DNA templates in this study to demonstrate that transcription in vitro from the P1 and P2 promoters of the osmoresponsive proU operon of Escherichia coli is preferentially mediated by the sigma(s) and sigma70-bearing RNA polymerase holoenzymes, res …
We have used supercoiled DNA templates in this study to demonstrate that transcription in vitro from the P1 and P2 promoters of the osmoresp …
Promoter selectivity of Escherichia coli RNA polymerase E sigma 70 and E sigma 38 holoenzymes. Effect of DNA supercoiling.
Kusano S, Ding Q, Fujita N, Ishihama A. Kusano S, et al. J Biol Chem. 1996 Jan 26;271(4):1998-2004. doi: 10.1074/jbc.271.4.1998. J Biol Chem. 1996. PMID: 8567650
We thus propose that the selective transcription of stationary-specific genes by E sigma 38 holoenzyme requires either a specific reaction condition(s) or a specific factor(s) such as template DNA with low superhelical density....
We thus propose that the selective transcription of stationary-specific genes by E sigma 38 holoenzyme requires either a specific reaction c …
Crosslinking reactions of 4-amino-6-oxo-2-vinylpyrimidine with guanine derivatives and structural analysis of the adducts.
Kusano S, Ishiyama S, Lam SL, Mashima T, Katahira M, Miyamoto K, Aida M, Nagatsugi F. Kusano S, et al. Nucleic Acids Res. 2015 Sep 18;43(16):7717-30. doi: 10.1093/nar/gkv797. Epub 2015 Aug 5. Nucleic Acids Res. 2015. PMID: 26245348 Free PMC article.
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