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Advanced Characterization of DNA Molecules in rAAV Vector Preparations by Single-stranded Virus Next-generation Sequencing.
Lecomte E, Tournaire B, Cogné B, Dupont JB, Lindenbaum P, Martin-Fontaine M, Broucque F, Robin C, Hebben M, Merten OW, Blouin V, François A, Redon R, Moullier P, Léger A. Lecomte E, et al. Among authors: leger a. Mol Ther Nucleic Acids. 2015 Oct 27;4(10):e260. doi: 10.1038/mtna.2015.32. Mol Ther Nucleic Acids. 2015. PMID: 26506038 Free PMC article.
Short-lived recombinant adeno-associated virus transgene expression in dystrophic muscle is associated with oxidative damage to transgene mRNA.
Dupont JB, Tournaire B, Georger C, Marolleau B, Jeanson-Leh L, Ledevin M, Lindenbaum P, Lecomte E, Cogné B, Dubreil L, Larcher T, Gjata B, Van Wittenberghe L, Le Guiner C, Penaud-Budloo M, Snyder RO, Moullier P, Léger A. Dupont JB, et al. Among authors: leger a. Mol Ther Methods Clin Dev. 2015 Apr 8;2:15010. doi: 10.1038/mtm.2015.10. eCollection 2015. Mol Ther Methods Clin Dev. 2015. PMID: 26029721 Free PMC article.
Accurate Identification and Quantification of DNA Species by Next-Generation Sequencing in Adeno-Associated Viral Vectors Produced in Insect Cells.
Penaud-Budloo M, Lecomte E, Guy-Duché A, Saleun S, Roulet A, Lopez-Roques C, Tournaire B, Cogné B, Léger A, Blouin V, Lindenbaum P, Moullier P, Ayuso E. Penaud-Budloo M, et al. Among authors: leger a. Hum Gene Ther Methods. 2017 Jun;28(3):148-162. doi: 10.1089/hgtb.2016.185. Epub 2017 Apr 21. Hum Gene Ther Methods. 2017. PMID: 28463571
AAV8 locoregional delivery induces long-term expression of an immunogenic transgene in macaques despite persisting local inflammation.
Gernoux G, Guilbaud M, Devaux M, Journou M, Pichard V, Jaulin N, Léger A, Le Duff J, Deschamps JY, Le Guiner C, Moullier P, Cherel Y, Adjali O. Gernoux G, et al. Among authors: leger a. Mol Ther Methods Clin Dev. 2021 Feb 6;20:660-674. doi: 10.1016/j.omtm.2021.02.003. eCollection 2021 Mar 12. Mol Ther Methods Clin Dev. 2021. PMID: 33718516 Free PMC article.
Nanopore RNA Sequencing Analysis.
Leonardi T, Leger A. Leonardi T, et al. Among authors: leger a. Methods Mol Biol. 2021;2284:569-578. doi: 10.1007/978-1-0716-1307-8_31. Methods Mol Biol. 2021. PMID: 33835464
346 results