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Native Elution of Yeast Protein Complexes Obtained by Affinity Capture.
LaCava J, Fernandez-Martinez J, Rout MP. LaCava J, et al. Cold Spring Harb Protoc. 2016 Jul 1;2016(7). doi: 10.1101/pdb.prot087940. Cold Spring Harb Protoc. 2016. PMID: 27371597
Improved native isolation of endogenous Protein A-tagged protein complexes.
LaCava J, Chandramouli N, Jiang H, Rout MP. LaCava J, et al. Biotechniques. 2013 Apr;54(4):213-6. doi: 10.2144/000114012. Biotechniques. 2013. PMID: 23581468 Free PMC article.
Affinity proteomics to study endogenous protein complexes: pointers, pitfalls, preferences and perspectives.
LaCava J, Molloy KR, Taylor MS, Domanski M, Chait BT, Rout MP. LaCava J, et al. Biotechniques. 2015 Mar 1;58(3):103-19. doi: 10.2144/000114262. eCollection 2015 Mar. Biotechniques. 2015. PMID: 25757543 Free PMC article.
Rapid, optimized interactomic screening.
Hakhverdyan Z, Domanski M, Hough LE, Oroskar AA, Oroskar AR, Keegan S, Dilworth DJ, Molloy KR, Sherman V, Aitchison JD, Fenyƶ D, Chait BT, Jensen TH, Rout MP, LaCava J. Hakhverdyan Z, et al. Nat Methods. 2015 Jun;12(6):553-60. doi: 10.1038/nmeth.3395. Epub 2015 May 4. Nat Methods. 2015. PMID: 25938370 Free PMC article.
Characterization of L1-Ribonucleoprotein Particles.
Taylor MS, LaCava J, Dai L, Mita P, Burns KH, Rout MP, Boeke JD. Taylor MS, et al. Methods Mol Biol. 2016;1400:311-38. doi: 10.1007/978-1-4939-3372-3_20. Methods Mol Biol. 2016. PMID: 26895062 Free PMC article.
Optimized Affinity Capture of Yeast Protein Complexes.
LaCava J, Fernandez-Martinez J, Hakhverdyan Z, Rout MP. LaCava J, et al. Cold Spring Harb Protoc. 2016 Jul 1;2016(7). doi: 10.1101/pdb.prot087932. Cold Spring Harb Protoc. 2016. PMID: 27371596
Protein Complex Purification by Affinity Capture.
LaCava J, Fernandez-Martinez J, Hakhverdyan Z, Rout MP. LaCava J, et al. Cold Spring Harb Protoc. 2016 Jul 1;2016(7). doi: 10.1101/pdb.top077545. Cold Spring Harb Protoc. 2016. PMID: 27371601
Purification and analysis of endogenous human RNA exosome complexes.
Domanski M, Upla P, Rice WJ, Molloy KR, Ketaren NE, Stokes DL, Jensen TH, Rout MP, LaCava J. Domanski M, et al. RNA. 2016 Sep;22(9):1467-75. doi: 10.1261/rna.057760.116. Epub 2016 Jul 11. RNA. 2016. PMID: 27402899 Free PMC article.
Protein Complex Affinity Capture from Cryomilled Mammalian Cells.
LaCava J, Jiang H, Rout MP. LaCava J, et al. J Vis Exp. 2016 Dec 9;(118):54518. doi: 10.3791/54518. J Vis Exp. 2016. PMID: 28060343 Free PMC article.
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