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Substitution of Arg-244 by Cys or Ser in SHV-1 and SHV-5 beta-lactamases confers resistance to mechanism-based inhibitors and reduces catalytic efficiency of the enzymes.
Giakkoupi P, Tzelepi E, Legakis NJ, Tzouvelekis LS. Giakkoupi P, et al. FEMS Microbiol Lett. 1998 Mar 1;160(1):49-54. doi: 10.1111/j.1574-6968.1998.tb12889.x. FEMS Microbiol Lett. 1998. PMID: 9495011
In vitro activity of tigecycline against 2423 clinical isolates and comparison of the available interpretation breakpoints.
Papaparaskevas J, Tzouvelekis LS, Tsakris A, Pittaras TE, Legakis NJ; Hellenic Tigecycline Study Group. Papaparaskevas J, et al. Diagn Microbiol Infect Dis. 2010 Feb;66(2):187-94. doi: 10.1016/j.diagmicrobio.2009.09.012. Diagn Microbiol Infect Dis. 2010. PMID: 19836184
In vitro activities of tigecycline against recently isolated Gram-negative anaerobic bacteria in Greece, including metronidazole-resistant strains.
Katsandri A, Avlamis A, Pantazatou A, Petrikkos GL, Legakis NJ, Papaparaskevas J; Hellenic Study Group for Gram-Negative Anaerobic Bacteria. Katsandri A, et al. Diagn Microbiol Infect Dis. 2006 Jul;55(3):231-6. doi: 10.1016/j.diagmicrobio.2006.01.022. Epub 2006 Apr 19. Diagn Microbiol Infect Dis. 2006. PMID: 16626904 Clinical Trial.
Activity of cefiderocol (S-649266) against carbapenem-resistant Gram-negative bacteria collected from inpatients in Greek hospitals.
Falagas ME, Skalidis T, Vardakas KZ, Legakis NJ; Hellenic Cefiderocol Study Group. Falagas ME, et al. Among authors: legakis nj. J Antimicrob Chemother. 2017 Jun 1;72(6):1704-1708. doi: 10.1093/jac/dkx049. J Antimicrob Chemother. 2017. PMID: 28369471
Moxifloxacin resistance is prevalent among Bacteroides and Prevotella species in Greece.
Papaparaskevas J, Pantazatou A, Katsandri A, Houhoula DP, Legakis NJ, Tsakris A, Avlamis A. Papaparaskevas J, et al. J Antimicrob Chemother. 2008 Jul;62(1):137-41. doi: 10.1093/jac/dkn134. Epub 2008 Apr 1. J Antimicrob Chemother. 2008. PMID: 18385145
Frequency and predictors of colonization of the respiratory tract by VIM-2-producing Pseudomonas aeruginosa in patients of a newly established intensive care unit.
Horianopoulou M, Legakis NJ, Kanellopoulou M, Lambropoulos S, Tsakris A, Falagas ME. Horianopoulou M, et al. J Med Microbiol. 2006 Oct;55(Pt 10):1435-1439. doi: 10.1099/jmm.0.46713-0. J Med Microbiol. 2006. PMID: 17005794
VIM-1 metallo-beta-lactamase in Acinetobacter baumannii.
Tsakris A, Ikonomidis A, Pournaras S, Tzouvelekis LS, Sofianou D, Legakis NJ, Maniatis AN. Tsakris A, et al. Emerg Infect Dis. 2006 Jun;12(6):981-3. doi: 10.3201/eid1206.051097. Emerg Infect Dis. 2006. PMID: 16707056 Free PMC article.
Klebsiella pneumoniae infections in Greek hospitals. Dissemination of plasmids encoding an SHV-5 type beta-lactamase.
Legakis NJ, Tzouvelekis LS, Hatzoudis G, Tzelepi E, Gourkou A, Pitt TL, Vatopoulos AC. Legakis NJ, et al. J Hosp Infect. 1995 Nov;31(3):177-87. doi: 10.1016/0195-6701(95)90064-0. J Hosp Infect. 1995. PMID: 8586786
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