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Methods for qPCR gene expression profiling applied to 1440 lymphoblastoid single cells.
Livak KJ, Wills QF, Tipping AJ, Datta K, Mittal R, Goldson AJ, Sexton DW, Holmes CC. Livak KJ, et al. Methods. 2013 Jan;59(1):71-9. doi: 10.1016/j.ymeth.2012.10.004. Epub 2012 Oct 16. Methods. 2013. PMID: 23079396 Free PMC article.
Quantitative PCR based expression analysis on a nanoliter scale using polymer nano-well chips.
Dahl A, Sultan M, Jung A, Schwartz R, Lange M, Steinwand M, Livak KJ, Lehrach H, Nyarsik L. Dahl A, et al. Biomed Microdevices. 2007 Jun;9(3):307-14. doi: 10.1007/s10544-006-9034-2. Biomed Microdevices. 2007. PMID: 17203381
Taking qPCR to a higher level: Analysis of CNV reveals the power of high throughput qPCR to enhance quantitative resolution.
Weaver S, Dube S, Mir A, Qin J, Sun G, Ramakrishnan R, Jones RC, Livak KJ. Weaver S, et al. Methods. 2010 Apr;50(4):271-6. doi: 10.1016/j.ymeth.2010.01.003. Epub 2010 Jan 15. Methods. 2010. PMID: 20079846
Multiplexing RT-PCR for the detection of multiple miRNA species in small samples.
Lao K, Xu NL, Yeung V, Chen C, Livak KJ, Straus NA. Lao K, et al. Biochem Biophys Res Commun. 2006 Apr 28;343(1):85-9. doi: 10.1016/j.bbrc.2006.02.106. Epub 2006 Feb 28. Biochem Biophys Res Commun. 2006. PMID: 16529715
Quantitative real-time RT-PCR analysis of PML-RAR alpha mRNA in acute promyelocytic leukemia: assessment of prognostic significance in adult patients from intergroup protocol 0129.
Gallagher RE, Yeap BY, Bi W, Livak KJ, Beaubier N, Rao S, Bloomfield CD, Appelbaum FR, Tallman MS, Slack JL, Willman CL. Gallagher RE, et al. Blood. 2003 Apr 1;101(7):2521-8. doi: 10.1182/blood-2002-05-1357. Epub 2002 Dec 5. Blood. 2003. PMID: 12468436
Real-time quantification of microRNAs by stem-loop RT-PCR.
Chen C, Ridzon DA, Broomer AJ, Zhou Z, Lee DH, Nguyen JT, Barbisin M, Xu NL, Mahuvakar VR, Andersen MR, Lao KQ, Livak KJ, Guegler KJ. Chen C, et al. Nucleic Acids Res. 2005 Nov 27;33(20):e179. doi: 10.1093/nar/gni178. Nucleic Acids Res. 2005. PMID: 16314309 Free PMC article.
Analyzing real-time PCR data by the comparative C(T) method.
Schmittgen TD, Livak KJ. Schmittgen TD, et al. Nat Protoc. 2008;3(6):1101-8. doi: 10.1038/nprot.2008.73. Nat Protoc. 2008. PMID: 18546601
Methylation-dependent fragment separation: direct detection of DNA methylation by capillary electrophoresis of PCR products from bisulfite-converted genomic DNA.
Boyd VL, Moody KI, Karger AE, Livak KJ, Zon G, Burns JW. Boyd VL, et al. Anal Biochem. 2006 Jul 15;354(2):266-73. doi: 10.1016/j.ab.2006.04.009. Epub 2006 May 6. Anal Biochem. 2006. PMID: 16725101
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