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A Protein Epitope Signature Tag (PrEST) library allows SILAC-based absolute quantification and multiplexed determination of protein copy numbers in cell lines.
Zeiler M, Straube WL, Lundberg E, Uhlen M, Mann M. Zeiler M, et al. Mol Cell Proteomics. 2012 Mar;11(3):O111.009613. doi: 10.1074/mcp.O111.009613. Epub 2011 Sep 30. Mol Cell Proteomics. 2012. PMID: 21964433 Free PMC article.
These PrESTs are expressed recombinantly in E. coli and they consist of a short and unique region of the protein of interest as well as purification and solubility tags. ...
These PrESTs are expressed recombinantly in E. coli and they consist of a short and unique region of the protein of interest as well …
The correlation between cellular size and protein expression levels--normalization for global protein profiling.
Lundberg E, Gry M, Oksvold P, Kononen J, Andersson-Svahn H, Pontén F, Uhlén M, Asplund A. Lundberg E, et al. J Proteomics. 2008 Oct 7;71(4):448-60. doi: 10.1016/j.jprot.2008.06.014. Epub 2008 Jul 5. J Proteomics. 2008. PMID: 18656560
A genecentric Human Protein Atlas for expression profiles based on antibodies.
Berglund L, Björling E, Oksvold P, Fagerberg L, Asplund A, Szigyarto CA, Persson A, Ottosson J, Wernérus H, Nilsson P, Lundberg E, Sivertsson A, Navani S, Wester K, Kampf C, Hober S, Pontén F, Uhlén M. Berglund L, et al. Mol Cell Proteomics. 2008 Oct;7(10):2019-27. doi: 10.1074/mcp.R800013-MCP200. Mol Cell Proteomics. 2008. PMID: 18669619 Review.
Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
Jakobsen L, Vanselow K, Skogs M, Toyoda Y, Lundberg E, Poser I, Falkenby LG, Bennetzen M, Westendorf J, Nigg EA, Uhlen M, Hyman AA, Andersen JS. Jakobsen L, et al. EMBO J. 2011 Apr 20;30(8):1520-35. doi: 10.1038/emboj.2011.63. Epub 2011 Mar 11. EMBO J. 2011. PMID: 21399614 Free PMC article.
Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization.
Ahmad Y, Boisvert FM, Lundberg E, Uhlen M, Lamond AI. Ahmad Y, et al. Mol Cell Proteomics. 2012 Mar;11(3):M111.013680. doi: 10.1074/mcp.M111.013680. Epub 2011 Oct 16. Mol Cell Proteomics. 2012. PMID: 22002106 Free PMC article.
Antibody-based protein profiling of the human chromosome 21.
Uhlén M, Oksvold P, Älgenäs C, Hamsten C, Fagerberg L, Klevebring D, Lundberg E, Odeberg J, Pontén F, Kondo T, Sivertsson Å. Uhlén M, et al. Mol Cell Proteomics. 2012 Mar;11(3):M111.013458. doi: 10.1074/mcp.M111.013458. Epub 2011 Oct 31. Mol Cell Proteomics. 2012. PMID: 22042635 Free PMC article.
Identification of autophagosome-associated proteins and regulators by quantitative proteomic analysis and genetic screens.
Dengjel J, Høyer-Hansen M, Nielsen MO, Eisenberg T, Harder LM, Schandorff S, Farkas T, Kirkegaard T, Becker AC, Schroeder S, Vanselow K, Lundberg E, Nielsen MM, Kristensen AR, Akimov V, Bunkenborg J, Madeo F, Jäättelä M, Andersen JS. Dengjel J, et al. Mol Cell Proteomics. 2012 Mar;11(3):M111.014035. doi: 10.1074/mcp.M111.014035. Epub 2012 Feb 6. Mol Cell Proteomics. 2012. PMID: 22311637 Free PMC article.
Initial quantitative proteomic map of 28 mouse tissues using the SILAC mouse.
Geiger T, Velic A, Macek B, Lundberg E, Kampf C, Nagaraj N, Uhlen M, Cox J, Mann M. Geiger T, et al. Mol Cell Proteomics. 2013 Jun;12(6):1709-22. doi: 10.1074/mcp.M112.024919. Epub 2013 Feb 22. Mol Cell Proteomics. 2013. PMID: 23436904 Free PMC article.
Centrosome isolation and analysis by mass spectrometry-based proteomics.
Jakobsen L, Schrøder JM, Larsen KM, Lundberg E, Andersen JS. Jakobsen L, et al. Methods Enzymol. 2013;525:371-93. doi: 10.1016/B978-0-12-397944-5.00018-3. Methods Enzymol. 2013. PMID: 23522479
Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics.
Fagerberg L, Hallström BM, Oksvold P, Kampf C, Djureinovic D, Odeberg J, Habuka M, Tahmasebpoor S, Danielsson A, Edlund K, Asplund A, Sjöstedt E, Lundberg E, Szigyarto CA, Skogs M, Takanen JO, Berling H, Tegel H, Mulder J, Nilsson P, Schwenk JM, Lindskog C, Danielsson F, Mardinoglu A, Sivertsson A, von Feilitzen K, Forsberg M, Zwahlen M, Olsson I, Navani S, Huss M, Nielsen J, Ponten F, Uhlén M. Fagerberg L, et al. Mol Cell Proteomics. 2014 Feb;13(2):397-406. doi: 10.1074/mcp.M113.035600. Epub 2013 Dec 5. Mol Cell Proteomics. 2014. PMID: 24309898 Free PMC article.
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