Genetic code expansion in the mouse brain

Nat Chem Biol. 2016 Oct;12(10):776-778. doi: 10.1038/nchembio.2160. Epub 2016 Aug 29.

Abstract

Site-specific incorporation of non-natural amino acids into proteins, via genetic code expansion with pyrrolysyl tRNA synthetase (PylRS) and tRNA(Pyl)CUA pairs (and their evolved derivatives) from Methanosarcina sp., forms the basis of powerful approaches to probe and control protein function in cells and invertebrate organisms. Here we demonstrate that adeno-associated viral delivery of these pairs enables efficient genetic code expansion in primary neuronal culture, organotypic brain slices and the brains of live mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / chemistry*
  • Amino Acids / genetics*
  • Amino Acids / metabolism
  • Amino Acyl-tRNA Synthetases / metabolism*
  • Animals
  • Brain / cytology*
  • Brain / metabolism*
  • Dependovirus / genetics
  • Genetic Code / genetics*
  • Methanosarcina / genetics
  • Mice
  • Molecular Structure
  • RNA, Transfer / genetics*
  • RNA, Transfer / metabolism

Substances

  • Amino Acids
  • RNA, Transfer
  • Amino Acyl-tRNA Synthetases

Associated data

  • PubChem-Substance/316891612
  • PubChem-Substance/316891613
  • PubChem-Substance/316891614
  • PubChem-Substance/316891615
  • PubChem-Substance/316891616
  • PubChem-Substance/316891617