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Creating bacterial strains from genomes that have been cloned and engineered in yeast.
Lartigue C, Vashee S, Algire MA, Chuang RY, Benders GA, Ma L, Noskov VN, Denisova EA, Gibson DG, Assad-Garcia N, Alperovich N, Thomas DW, Merryman C, Hutchison CA 3rd, Smith HO, Venter JC, Glass JI. Lartigue C, et al. Among authors: ma l. Science. 2009 Sep 25;325(5948):1693-6. doi: 10.1126/science.1173759. Epub 2009 Aug 20. Science. 2009. PMID: 19696314
Creation of a bacterial cell controlled by a chemically synthesized genome.
Gibson DG, Glass JI, Lartigue C, Noskov VN, Chuang RY, Algire MA, Benders GA, Montague MG, Ma L, Moodie MM, Merryman C, Vashee S, Krishnakumar R, Assad-Garcia N, Andrews-Pfannkoch C, Denisova EA, Young L, Qi ZQ, Segall-Shapiro TH, Calvey CH, Parmar PP, Hutchison CA 3rd, Smith HO, Venter JC. Gibson DG, et al. Among authors: ma l. Science. 2010 Jul 2;329(5987):52-6. doi: 10.1126/science.1190719. Epub 2010 May 20. Science. 2010. PMID: 20488990
Sequence analysis of a complete 1.66 Mb Prochlorococcus marinus MED4 genome cloned in yeast.
Tagwerker C, Dupont CL, Karas BJ, Ma L, Chuang RY, Benders GA, Ramon A, Novotny M, Montague MG, Venepally P, Brami D, Schwartz A, Andrews-Pfannkoch C, Gibson DG, Glass JI, Smith HO, Venter JC, Hutchison CA 3rd. Tagwerker C, et al. Among authors: ma l. Nucleic Acids Res. 2012 Nov 1;40(20):10375-83. doi: 10.1093/nar/gks823. Epub 2012 Aug 31. Nucleic Acids Res. 2012. PMID: 22941652 Free PMC article.
Direct transfer of whole genomes from bacteria to yeast.
Karas BJ, Jablanovic J, Sun L, Ma L, Goldgof GM, Stam J, Ramon A, Manary MJ, Winzeler EA, Venter JC, Weyman PD, Gibson DG, Glass JI, Hutchison CA 3rd, Smith HO, Suzuki Y. Karas BJ, et al. Among authors: ma l. Nat Methods. 2013 May;10(5):410-2. doi: 10.1038/nmeth.2433. Epub 2013 Mar 31. Nat Methods. 2013. PMID: 23542886 Free PMC article.
TREC-IN: gene knock-in genetic tool for genomes cloned in yeast.
Chandran S, Noskov VN, Segall-Shapiro TH, Ma L, Whiteis C, Lartigue C, Jores J, Vashee S, Chuang RY. Chandran S, et al. Among authors: ma l. BMC Genomics. 2014 Dec 24;15(1):1180. doi: 10.1186/1471-2164-15-1180. BMC Genomics. 2014. PMID: 25539750 Free PMC article.
Design and synthesis of a minimal bacterial genome.
Hutchison CA 3rd, Chuang RY, Noskov VN, Assad-Garcia N, Deerinck TJ, Ellisman MH, Gill J, Kannan K, Karas BJ, Ma L, Pelletier JF, Qi ZQ, Richter RA, Strychalski EA, Sun L, Suzuki Y, Tsvetanova B, Wise KS, Smith HO, Glass JI, Merryman C, Gibson DG, Venter JC. Hutchison CA 3rd, et al. Among authors: ma l. Science. 2016 Mar 25;351(6280):aad6253. doi: 10.1126/science.aad6253. Science. 2016. PMID: 27013737
One step engineering of the small-subunit ribosomal RNA using CRISPR/Cas9.
Kannan K, Tsvetanova B, Chuang RY, Noskov VN, Assad-Garcia N, Ma L, Hutchison Iii CA, Smith HO, Glass JI, Merryman C, Venter JC, Gibson DG. Kannan K, et al. Among authors: ma l. Sci Rep. 2016 Aug 4;6:30714. doi: 10.1038/srep30714. Sci Rep. 2016. PMID: 27489041 Free PMC article.
Removal of a Subset of Non-essential Genes Fully Attenuates a Highly Virulent Mycoplasma Strain.
Jores J, Ma L, Ssajjakambwe P, Schieck E, Liljander A, Chandran S, Stoffel MH, Cippa V, Arfi Y, Assad-Garcia N, Falquet L, Sirand-Pugnet P, Blanchard A, Lartigue C, Posthaus H, Labroussaa F, Vashee S. Jores J, et al. Among authors: ma l. Front Microbiol. 2019 Apr 3;10:664. doi: 10.3389/fmicb.2019.00664. eCollection 2019. Front Microbiol. 2019. PMID: 31001234 Free PMC article.
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