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The purification and characterization of the catalytic domain of Src expressed in Schizosaccharomyces pombe. Comparison of unphosphorylated and tyrosine phosphorylated species.
Weijland A, Neubauer G, Courtneidge SA, Mann M, Wierenga RK, Superti-Furga G. Weijland A, et al. Among authors: mann m. Eur J Biochem. 1996 Sep 15;240(3):756-64. doi: 10.1111/j.1432-1033.1996.0756h.x. Eur J Biochem. 1996. PMID: 8856081
The kcat values for phosphorylation of the Src substrate peptide are similar for the unphosphorylated and monophosphorylated Src-CD (50 min-1), but the apparent K(m) values differ significantly (approximately 3 microM and 10 microM, respectively). ...The apparent K(m
The kcat values for phosphorylation of the Src substrate peptide are similar for the unphosphorylated and monophosphorylated Src-CD (50 min- …
Detection of tyrosine phosphorylated peptides by precursor ion scanning quadrupole TOF mass spectrometry in positive ion mode.
Steen H, Küster B, Fernandez M, Pandey A, Mann M. Steen H, et al. Among authors: mann m. Anal Chem. 2001 Apr 1;73(7):1440-8. doi: 10.1021/ac001318c. Anal Chem. 2001. PMID: 11321292
The method utilizes the immonium ion of phosphotyrosine at m/z 216.043 for positive ion mode precursor ion scanning combined with the recently introduced Q2-pulsing function on quadrupole TOF mass spectrometers. ...
The method utilizes the immonium ion of phosphotyrosine at m/z 216.043 for positive ion mode precursor ion scanning combined with the …
Sequence tag identification of intact proteins by matching tanden mass spectral data against sequence data bases.
Mørtz E, O'Connor PB, Roepstorff P, Kelleher NL, Wood TD, McLafferty FW, Mann M. Mørtz E, et al. Among authors: mann m. Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8264-7. doi: 10.1073/pnas.93.16.8264. Proc Natl Acad Sci U S A. 1996. PMID: 8710858 Free PMC article.
Extending the sequence tag concept of Mann and Wilm for matching peptides, a partial amino acid sequence in the unknown is first identified from the mass differences of a series of fragment ions, and the mass position of this sequence is defined from molecular weight and t …
Extending the sequence tag concept of Mann and Wilm for matching peptides, a partial amino acid sequence in the unknown is first iden …
Use of mass spectrometric molecular weight information to identify proteins in sequence databases.
Mann M, Højrup P, Roepstorff P. Mann M, et al. Biol Mass Spectrom. 1993 Jun;22(6):338-45. doi: 10.1002/bms.1200220605. Biol Mass Spectrom. 1993. PMID: 8329463
Isoprenylation of the G protein gamma subunit is both necessary and sufficient for beta gamma dimer-mediated stimulation of phospholipase C.
Dietrich A, Brazil D, Jensen ON, Meister M, Schrader M, Moomaw JF, Mann M, Illenberger D, Gierschik P. Dietrich A, et al. Among authors: mann m. Biochemistry. 1996 Dec 3;35(48):15174-82. doi: 10.1021/bi960305j. Biochemistry. 1996. PMID: 8952464
., Meister, M., Brazil, D., Camps, M., & Gierschik, P. (1994) Eur. J. Biochem. 219, 171-178]. To examine whether isoprenylation of the gamma 1 subunit alone is sufficient for beta 1 gamma 1-mediated PLC beta 2 stimulation or whether any of the two subsequent mod …
., Meister, M., Brazil, D., Camps, M., & Gierschik, P. (1994) Eur. J. Biochem. 219, 171-178]. To examine whether isoprenyl …
A generic protein purification method for protein complex characterization and proteome exploration.
Rigaut G, Shevchenko A, Rutz B, Wilm M, Mann M, Séraphin B. Rigaut G, et al. Among authors: mann m. Nat Biotechnol. 1999 Oct;17(10):1030-2. doi: 10.1038/13732. Nat Biotechnol. 1999. PMID: 10504710
Automation of matrix-assisted laser desorption/ionization mass spectrometry using fuzzy logic feedback control.
Jensen ON, Mortensen P, Vorm O, Mann M. Jensen ON, et al. Among authors: mann m. Anal Chem. 1997 May 1;69(9):1706-14. doi: 10.1021/ac961189t. Anal Chem. 1997. PMID: 9145026
Identification of proteins in polyacrylamide gels by mass spectrometric peptide mapping combined with database search.
Mørtz E, Vorm O, Mann M, Roepstorff P. Mørtz E, et al. Among authors: mann m. Biol Mass Spectrom. 1994 May;23(5):249-61. doi: 10.1002/bms.1200230503. Biol Mass Spectrom. 1994. PMID: 8204681
A strategy for identifying gel-separated proteins in sequence databases by MS alone.
Shevchenko A, Wilm M, Vorm O, Jensen ON, Podtelejnikov AV, Neubauer G, Shevchenko A, Mortensen P, Mann M. Shevchenko A, et al. Among authors: mann m. Biochem Soc Trans. 1996 Aug;24(3):893-6. doi: 10.1042/bst0240893. Biochem Soc Trans. 1996. PMID: 8878870 No abstract available.
Analysis of proteins and proteomes by mass spectrometry.
Mann M, Hendrickson RC, Pandey A. Mann M, et al. Annu Rev Biochem. 2001;70:437-73. doi: 10.1146/annurev.biochem.70.1.437. Annu Rev Biochem. 2001. PMID: 11395414 Review.
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