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Specific photoaffinity-labeling of Tyr-50 on the heavy chain and of Tyr-32 on the light chain in the steroid combining site of a mouse monoclonal anti-estradiol antibody using C3-, C6-, and C7-linked 5-azido-2-nitrobenzoylamidoestradiol photoreagents.
Rolland de Ravel M, Blachère T, Delolme F, Dessalces G, Coulon S, Baty D, Grenot C, Mappus E, Cuilleron CY. Rolland de Ravel M, et al. Among authors: mappus e. Biochemistry. 2001 Dec 11;40(49):14907-20. doi: 10.1021/bi011174b. Biochemistry. 2001. PMID: 11732911
Characterization of Met-139 as the photolabeled amino acid residue in the steroid binding site of sex hormone binding globulin using delta 6 derivatives of either testosterone or estradiol as unsubstituted photoaffinity labeling reagents.
Grenot C, de Montard A, Blachère T, de Ravel MR, Mappus E, Cuilleron CY. Grenot C, et al. Among authors: mappus e. Biochemistry. 1992 Aug 25;31(33):7609-21. doi: 10.1021/bi00148a024. Biochemistry. 1992. PMID: 1510947
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