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Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h.
Chiu J, March PE, Lee R, Tillett D. Chiu J, et al. Among authors: march pe. Nucleic Acids Res. 2004 Dec 7;32(21):e174. doi: 10.1093/nar/gnh172. Nucleic Acids Res. 2004. PMID: 15585660 Free PMC article.
Site-directed, Ligase-Independent Mutagenesis (SLIM) for highly efficient mutagenesis of plasmids greater than 8kb.
Chiu J, Tillett D, Dawes IW, March PE. Chiu J, et al. Among authors: march pe. J Microbiol Methods. 2008 May;73(2):195-8. doi: 10.1016/j.mimet.2008.02.013. Epub 2008 Feb 26. J Microbiol Methods. 2008. PMID: 18387684
Coexpression of the subunits of T7 DNA polymerase from an artificial operon allows one-step purification of active gp5/Trx complex.
Chiu J, Tillett D, March PE. Chiu J, et al. Among authors: march pe. Protein Expr Purif. 2006 May;47(1):264-72. doi: 10.1016/j.pep.2005.10.016. Epub 2005 Nov 8. Protein Expr Purif. 2006. PMID: 16300964
Expression phenotypes suggest that Der participates in a specific, high affinity interaction with membranes.
Lee R, Aung-Htut MT, Kwik C, March PE. Lee R, et al. Among authors: march pe. Protein Expr Purif. 2011 Jul;78(1):102-12. doi: 10.1016/j.pep.2011.02.009. Epub 2011 Feb 24. Protein Expr Purif. 2011. PMID: 21354313
Mutation of Phe102 to Ser in the carboxyl terminal helix of Escherichia coli thioredoxin affects the stability and processivity of T7 DNA polymerase.
Chiu J, Tillett D, March PE. Chiu J, et al. Among authors: march pe. Proteins. 2006 Aug 1;64(2):477-85. doi: 10.1002/prot.20992. Proteins. 2006. PMID: 16671068
The function of conserved amino acid residues adjacent to the effector domain in elongation factor G.
Sharer JD, Koosha H, Church WB, March PE. Sharer JD, et al. Among authors: march pe. Proteins. 1999 Nov 1;37(2):293-302. Proteins. 1999. PMID: 10584074
Small clusters of divergent amino acids surrounding the effector domain mediate the varied phenotypes of EF-G and LepA expression.
Yaskowiak ES, March PE. Yaskowiak ES, et al. Among authors: march pe. Mol Microbiol. 1995 Mar;15(5):943-53. doi: 10.1111/j.1365-2958.1995.tb02363.x. Mol Microbiol. 1995. PMID: 7596295
Function of the universally conserved bacterial GTPases.
Caldon CE, March PE. Caldon CE, et al. Among authors: march pe. Curr Opin Microbiol. 2003 Apr;6(2):135-9. doi: 10.1016/s1369-5274(03)00037-7. Curr Opin Microbiol. 2003. PMID: 12732302 Review.
Evolution of a molecular switch: universal bacterial GTPases regulate ribosome function.
Caldon CE, Yoong P, March PE. Caldon CE, et al. Among authors: march pe. Mol Microbiol. 2001 Jul;41(2):289-97. doi: 10.1046/j.1365-2958.2001.02536.x. Mol Microbiol. 2001. PMID: 11489118 Free article. Review.
Identification and functional analysis of RNase E of Vibrio angustum S14 and two-hybrid analysis of its interaction partners.
Erce MA, Low JK, March PE, Wilkins MR, Takayama KM. Erce MA, et al. Among authors: march pe. Biochim Biophys Acta. 2009 Aug;1794(8):1107-14. doi: 10.1016/j.bbapap.2009.03.016. Epub 2009 Apr 2. Biochim Biophys Acta. 2009. PMID: 19345289
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