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Proteomic analysis in the identification of allergenic molecules.
Mari A, Ciardiello MA, Tamburrini M, Rasi C, Palazzo P. Mari A, et al. Expert Rev Proteomics. 2010 Oct;7(5):723-34. doi: 10.1586/epr.10.44. Expert Rev Proteomics. 2010. PMID: 20973644 Review.
The analysis includes the protein extract preparation, starting from the allergenic source, the separation of the proteins contained in a mixture and the detection, identification and characterization of IgE-binding molecules. ...A comparative analysis of biochemist …
The analysis includes the protein extract preparation, starting from the allergenic source, the separation of the proteins contained in a
Complementarity between microarray and immunoblot for the comparative evaluation of IgE repertoire of French and Italian cypress pollen allergic patients.
Shahali Y, Nicaise P, Brázdová A, Charpin D, Scala E, Mari A, Sutra JP, Chollet-Martin S, Sénéchal H, Poncet P. Shahali Y, et al. Among authors: mari a. Folia Biol (Praha). 2014;60(4):192-201. Folia Biol (Praha). 2014. PMID: 25152053
Patients allergic to Cupressus sempervirens pollen (Cups) (CPA) can be discriminated on the basis of the immunoglobulin E (IgE) binding to a basic 14 kDa protein (BP14) or to high-molecular-weight (HMW) glycoproteins only. ...BP14 sensitization is likely a marker of …
Patients allergic to Cupressus sempervirens pollen (Cups) (CPA) can be discriminated on the basis of the immunoglobulin E (IgE) binding to …
Proteomics plus genomics approaches in primary immunodeficiency: the case of immune dysregulation, polyendocrinopathy, enteropathy, X-linked (IPEX) syndrome.
Zennaro D, Scala E, Pomponi D, Caprini E, Arcelli D, Gambineri E, Russo G, Mari A. Zennaro D, et al. Among authors: mari a. Clin Exp Immunol. 2012 Jan;167(1):120-8. doi: 10.1111/j.1365-2249.2011.04492.x. Clin Exp Immunol. 2012. PMID: 22132891 Free PMC article.
A T cell-naive phenotype (CD62L(+) CD45R0(-)) associated with a reduction of both CD26 and CD7 expression and a TCR-Vbeta8 and 22 family expansions were found. B lymphocytes were mainly CD5(+) (B1) cells expressing a naive phenotype (tcl1(+) CD27(-)).
A T cell-naive phenotype (CD62L(+) CD45R0(-)) associated with a reduction of both CD26 and CD7 expression and a TCR-Vbe
Ovomucoid (Gal d 1) specific IgE detected by microarray system predict tolerability to boiled hen's egg and an increased risk to progress to multiple environmental allergen sensitisation.
Alessandri C, Zennaro D, Scala E, Ferrara R, Bernardi ML, Santoro M, Palazzo P, Mari A. Alessandri C, et al. Among authors: mari a. Clin Exp Allergy. 2012 Mar;42(3):441-50. doi: 10.1111/j.1365-2222.2011.03915.x. Epub 2011 Dec 14. Clin Exp Allergy. 2012. PMID: 22168465 Clinical Trial.
BACKGROUND: Egg allergy is a very common finding in early childhood. Detecting hen's egg (HE) allergy outgrowing and reintroduction of food containing egg is a task for the allergist. ...CONCLUSION AND CLINICAL RELEVANCE: The Gal d 1 IgE reactivity appears to be …
BACKGROUND: Egg allergy is a very common finding in early childhood. Detecting hen's egg (HE) allergy outgrowing and reintroduction o …
When does a protein become an allergen? Searching for a dynamic definition based on most advanced technology tools.
Mari A. Mari A. Clin Exp Allergy. 2008 Jul;38(7):1089-94. doi: 10.1111/j.1365-2222.2008.03011.x. Clin Exp Allergy. 2008. PMID: 18477011 Free PMC article. Review.
The need to organize this information leads to the most important question 'when does a protein become an allergen?' In this article, I try to address this question by reviewing a few basic concepts of the immunology of IgE-mediated diseases, reporting on the curren …
The need to organize this information leads to the most important question 'when does a protein become an allergen?' In this article, …
Recombinant cypress allergens.
Mari A. Mari A. Allerg Immunol (Paris). 2000 Mar;32(3):98-100. Allerg Immunol (Paris). 2000. PMID: 10815235
These molecules can be divided in four groups depending on their biologic activity: he Pectate lyases (rCry j 1, rCha o 1, rJun a 1); the Polygalacturonase (rCry j 2, rCha o 2); the Thaumatin-like pathogenesis-related protein (rJun a 3); the Calcium-binding protein …
These molecules can be divided in four groups depending on their biologic activity: he Pectate lyases (rCry j 1, rCha o 1, rJun a 1); …
Role of carbohydrate moieties in IgE binding to allergenic components of Cupressus arizonica pollen extract.
Afferni C, Iacovacci P, Barletta B, Di Felice G, Tinghino R, Mari A, Pini C. Afferni C, et al. Among authors: mari a. Clin Exp Allergy. 1999 Aug;29(8):1087-94. doi: 10.1046/j.1365-2222.1999.00590.x. Clin Exp Allergy. 1999. PMID: 10457113
BACKGROUND: A reduction of IgE immunoreactivity after periodate-treatment has been previously reported for various glycoprotein allergens. OBJECTIVE: The aim of this study was to investigate the role of glycan moiety of a C. arizonica extract in the binding of patie …
BACKGROUND: A reduction of IgE immunoreactivity after periodate-treatment has been previously reported for various glycoprotein aller …
Use of monoclonal antibodies in the standardization of Parietaria judaica allergenic extracts.
Afferni C, Pini C, Tinghino R, Palumbo S, Biocca MM, Bruno G, Mari A, Di Felice G. Afferni C, et al. Among authors: mari a. Biologicals. 1995 Sep;23(3):239-47. doi: 10.1006/biol.1995.0040. Biologicals. 1995. PMID: 8527125
These two antibodies, either individually or combined, were used to develop an ELISA-inhibition system using a reference Parietaria judaica extract (in-house Reference preparation, IHR). ...A good reproducibility, evaluated both at the level of 50% inhibition values …
These two antibodies, either individually or combined, were used to develop an ELISA-inhibition system using a reference Parietaria j …
Allergens of Arizona cypress (Cupressus arizonica) pollen: characterization of the pollen extract and identification of the allergenic components.
Di Felice G, Caiaffa MF, Bariletto G, Afferni C, Di Paola R, Mari A, Palumbo S, Tinghino R, Sallusto F, Tursi A, et al. Di Felice G, et al. Among authors: mari a. J Allergy Clin Immunol. 1994 Sep;94(3 Pt 1):547-55. doi: 10.1016/0091-6749(94)90212-7. J Allergy Clin Immunol. 1994. PMID: 8083460
Species of the Cupressaceae family are an important cause of respiratory allergies in countries with a Mediterranean climate. ...IgE reactivity was restricted to six components, whereas IgG binding showed a more complex pattern. A 43 kd component, predominant …
Species of the Cupressaceae family are an important cause of respiratory allergies in countries with a Mediterranean climate. ...IgE …
Peamaclein--a new peach allergenic protein: similarities, differences and misleading features compared to Pru p 3.
Tuppo L, Alessandri C, Pomponi D, Picone D, Tamburrini M, Ferrara R, Petriccione M, Mangone I, Palazzo P, Liso M, Giangrieco I, Crescenzo R, Bernardi ML, Zennaro D, Helmer-Citterich M, Mari A, Ciardiello MA. Tuppo L, et al. Among authors: mari a. Clin Exp Allergy. 2013 Jan;43(1):128-40. doi: 10.1111/cea.12028. Clin Exp Allergy. 2013. PMID: 23278887 Clinical Trial.
OBJECTIVE: To identify and characterize a new peach allergen causing a clinical picture similar to that of Pru p 3. METHODS: Patients were selected on the basis of their severe clinical reactivity and negative results to a panel of peach allergens available o …
OBJECTIVE: To identify and characterize a new peach allergen causing a clinical picture similar to that of Pru p 3. METHODS: P …
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