The purpose of this study was to determine collegiate athletes' knowledge, attitudes, and behaviors concerning hydration and fluid replacement. A survey containing questions pertaining to demographics and knowledge, attitude, and behavior on hydration and fluid replacement was distributed to the athletes during team meetings and practices. A total of 139 out of 171 (81.3%) athletes participated in the study. The mean age of the athletes was 19.8 y. The mean score for knowledge, attitude, and behavior was 13.9 +/-1.8, 9.8 +/- 2.2, and 12.4 +/- 2.5, respectively, with higher scores indicating positive hydration knowledge, attitudes, and behaviors. Significant positive correlation was observed between knowledge, attitude, and behavior scores (P < 0.05). Significant difference (P < 0.05) was observed in the reported hydration behaviors between skilled (11.79 +/- 2.08) and endurance (12.71 +/- 2.63) athletes. Most athletes correctly answered the general hydration questions on the survey, but the majority did not correctly answer statements in regards to National Athletic Trainers' Association (NATA) and the American College of Sports Medicine (ACSM) position stands and lacked knowledge regarding appropriate use of sports drink. The results of this study identify specific areas of education for athletes with regards to hydration.