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The NADPH-linked acetoacetyl-CoA reductase from Zoogloea ramigera. Characterization and mechanistic studies of the cloned enzyme over-produced in Escherichia coli.
Ploux O, Masamune S, Walsh CT. Ploux O, et al. Eur J Biochem. 1988 May 16;174(1):177-82. doi: 10.1111/j.1432-1033.1988.tb14079.x. Eur J Biochem. 1988. PMID: 3286259
It has a Km of 2 microM and a kcat/Km of 1.8 X 10(8) M-1 s-1 for acetoacetyl-CoA; it is inhibited by acetoacetyl-CoA above 10 microM. ...
It has a Km of 2 microM and a kcat/Km of 1.8 X 10(8) M-1 s-1 for acetoacetyl-CoA; it is inhibited by acetoacetyl-CoA above 10 microM. …
Mechanistic studies on beta-ketoacyl thiolase from Zoogloea ramigera: identification of the active-site nucleophile as Cys89, its mutation to Ser89, and kinetic and thermodynamic characterization of wild-type and mutant enzymes.
Thompson S, Mayerl F, Peoples OP, Masamune S, Sinskey AJ, Walsh CT. Thompson S, et al. Biochemistry. 1989 Jul 11;28(14):5735-42. doi: 10.1021/bi00440a006. Biochemistry. 1989. PMID: 2775734
Thiolase proceeds via covalent catalysis involving an acetyl-S-enzyme. The active-site thiol nucleophile is identified as Cys89 by acetylation with [14C]acetyl-CoA, rapid denaturation, tryptic digestion, and sequencing of the labeled peptide. ...
Thiolase proceeds via covalent catalysis involving an acetyl-S-enzyme. The active-site thiol nucleophile is identified as Cys89 by ac …
Biosynthetic thiolase from Zoogloea ramigera. Mutagenesis of the putative active-site base Cys-378 to Ser-378 changes the partitioning of the acetyl S-enzyme intermediate.
Williams SF, Palmer MA, Peoples OP, Walsh CT, Sinskey AJ, Masamune S. Williams SF, et al. J Biol Chem. 1992 Aug 15;267(23):16041-3. J Biol Chem. 1992. PMID: 1353760
Analysis of the acetyl S-enzyme intermediate partitioning, that is capture of the acetyl enzyme by 1) the thiolate of coenzyme A relative to 2) the C-2 carbanion of acetyl-CoA, is changed to favor reaction 2 in the case of the Ser-378 mutant enzyme....
Analysis of the acetyl S-enzyme intermediate partitioning, that is capture of the acetyl enzyme by 1) the thiolate of coenzyme A rela …
Biosynthetic thiolase from Zoogloea ramigera. Evidence for a mechanism involving Cys-378 as the active site base.
Palmer MA, Differding E, Gamboni R, Williams SF, Peoples OP, Walsh CT, Sinskey AJ, Masamune S. Palmer MA, et al. J Biol Chem. 1991 May 5;266(13):8369-75. J Biol Chem. 1991. PMID: 1673680
Biosynthetic thiolase from Zoogloea ramigera was inactivated with a mechanism-based inactivator, 3-pentynoyl-S-pantetheine-11-pivalate (3-pentynoyl-SPP) where K1 = 1.25 mM and kinact = 0.26 min-1, 2,3-pentadienoyl-SPP obtained from nonenzymatic rearrangement of 3-pentynoyl …
Biosynthetic thiolase from Zoogloea ramigera was inactivated with a mechanism-based inactivator, 3-pentynoyl-S-pantetheine-11-pivalat …
Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity.
Gerngross TU, Snell KD, Peoples OP, Sinskey AJ, Csuhai E, Masamune S, Stubbe J. Gerngross TU, et al. Biochemistry. 1994 Aug 9;33(31):9311-20. doi: 10.1021/bi00197a035. Biochemistry. 1994. PMID: 8049232
An extracellular poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis.
Tanio T, Fukui T, Shirakura Y, Saito T, Tomita K, Kaiho T, Masamune S. Tanio T, et al. Eur J Biochem. 1982 May;124(1):71-7. doi: 10.1111/j.1432-1033.1982.tb05907.x. Eur J Biochem. 1982. PMID: 7084231
Amphotericin B. Synthesis of its aglycone (amphoteronolide B) and degradation.
Masamune S. Masamune S. Ann N Y Acad Sci. 1988;544:168-79. doi: 10.1111/j.1749-6632.1988.tb40399.x. Ann N Y Acad Sci. 1988. PMID: 3214066 No abstract available.
Biosynthetic thiolase from zoogloea ramigera. I. Preliminary characterization and analysis of proton transfer reaction.
Davis JT, Moore RN, Imperiali B, Pratt AJ, Kobayashi K, Masamune S, Sinskey AJ, Walsh CT, Fukui T, Tomita K. Davis JT, et al. J Biol Chem. 1987 Jan 5;262(1):82-9. J Biol Chem. 1987. PMID: 2878927
Synthetic analogs of acetoacetyl-S-CoA, modified in the CoA moiety, were prepared and tested, and acetoacetyl-S-pantetheine 11-pivalate 1 was shown to have a kcat/Km of 6.4 X 10(6) M-1 s-1, comparable to the kcat/Km of 2 X 10(7) M-1 s-1 for acetoacetyl …
Synthetic analogs of acetoacetyl-S-CoA, modified in the CoA moiety, were prepared and tested, and acetoacetyl-S-pantetheine 11 …
Degradation of poly(3-hydroxybutyrate) by poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis T1.
Shirakura Y, Fukui T, Saito T, Okamoto Y, Narikawa T, Koide K, Tomita K, Takemasa T, Masamune S. Shirakura Y, et al. Biochim Biophys Acta. 1986 Jan 15;880(1):46-53. doi: 10.1016/0304-4165(86)90118-2. Biochim Biophys Acta. 1986. PMID: 3942778
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