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LC-MS/MS-based enzyme assay for lysosomal acid lipase using dried blood spots.
Ohira M, Barr M, Okuyama T, Mashima R. Ohira M, et al. Mol Genet Metab Rep. 2022 Aug 26;33:100913. doi: 10.1016/j.ymgmr.2022.100913. eCollection 2022 Dec. Mol Genet Metab Rep. 2022. PMID: 36065451 Free PMC article.
Due to the presence of multiple lipases in DBS, previous studies measured LAL enzyme activity in the presence of Lalistat-2, an established LAL-specific inhibitor (Hamilton J et al Chim Clin Acta (2012) 413:1207-1210). Alternatively, a novel substrate specific for LAL has …
Due to the presence of multiple lipases in DBS, previous studies measured LAL enzyme activity in the presence of Lalistat-2, an established …
Specific Substrate for the Assay of Lysosomal Acid Lipase.
Masi S, Chennamaneni N, Turecek F, Scott CR, Gelb MH. Masi S, et al. Clin Chem. 2018 Apr;64(4):690-696. doi: 10.1373/clinchem.2017.282251. Epub 2018 Jan 16. Clin Chem. 2018. PMID: 29339442 Free PMC article.