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Transcriptionally regulated immortalization overcomes side effects of temperature-sensitive SV40 large T antigen.
May T, Wirth D, Hauser H, Mueller PP. May T, et al. Biochem Biophys Res Commun. 2005 Feb 18;327(3):734-41. doi: 10.1016/j.bbrc.2004.12.065. Biochem Biophys Res Commun. 2005. PMID: 15649408
The temperature-sensitive mutant of the SV40 virus large T antigen (TAg) tsA58 is frequently employed for the conditional immortalization of primary cells. By increasing the temperature to 39 degrees C, the activity of the mutant TAg is reduced and the status of such cells …
The temperature-sensitive mutant of the SV40 virus large T antigen (TAg) tsA58 is frequently employed for the conditional immortaliza …
Expansion of functional personalized cells with specific transgene combinations.
Lipps C, Klein F, Wahlicht T, Seiffert V, Butueva M, Zauers J, Truschel T, Luckner M, Köster M, MacLeod R, Pezoldt J, Hühn J, Yuan Q, Müller PP, Kempf H, Zweigerdt R, Dittrich-Breiholz O, Pufe T, Beckmann R, Drescher W, Riancho J, Sañudo C, Korff T, Opalka B, Rebmann V, Göthert JR, Alves PM, Ott M, Schucht R, Hauser H, Wirth D, May T. Lipps C, et al. Among authors: may t. Nat Commun. 2018 Mar 8;9(1):994. doi: 10.1038/s41467-018-03408-4. Nat Commun. 2018. PMID: 29520052 Free PMC article.
1,211 results