Nationwide Molecular Epidemiology of Measles Virus in Japan Between 2008 and 2017

Fumio Seki; Masahiro Miyoshi; Tatsuya Ikeda; Haruna Nishijima; Miwako Saikusa; Masae Itamochi; Hiroko Minagawa; Takako Kurata; Rei Ootomo; Jumboku Kajiwara; Takashi Kato; Katsuhiro Komase; Keiko Tanaka-Taya; Tomimasa Sunagawa; Kazunori Oishi; Nobuhiko Okabe; Hirokazu Kimura; Shigeru Suga; Kunihisa Kozawa; Noriyuki Otsuki; Yoshio Mori; Komei Shirabe; Makoto Takeda; Measles Virus Surveillance Group of Japan; Technical Support Team for Measles Control in Japan

doi:10.3389/fmicb.2019.01470

Nationwide Molecular Epidemiology of Measles Virus in Japan Between 2008 and 2017

Front Microbiol. 2019 Jul 4:10:1470. doi: 10.3389/fmicb.2019.01470. eCollection 2019.

Authors

Fumio Seki¹, Masahiro Miyoshi², Tatsuya Ikeda³, Haruna Nishijima⁴, Miwako Saikusa⁵, Masae Itamochi⁶, Hiroko Minagawa⁷, Takako Kurata⁸, Rei Ootomo⁹, Jumboku Kajiwara¹⁰, Takashi Kato¹¹, Katsuhiro Komase¹², Keiko Tanaka-Taya¹², Tomimasa Sunagawa¹², Kazunori Oishi¹², Nobuhiko Okabe¹³, Hirokazu Kimura¹⁴, Shigeru Suga¹⁵, Kunihisa Kozawa¹⁶, Noriyuki Otsuki¹, Yoshio Mori¹, Komei Shirabe¹⁷, Makoto Takeda¹; Measles Virus Surveillance Group of Japan; Technical Support Team for Measles Control in Japan

Collaborators

Rika Komagome, Asami Ohnishi, Hiroyuki Saito, Mie Sasaki, Kenichi Komabayashi, Atsuko Kanari, Tsutomu Tamura, Kazunari Yamamoto, Kanako Ishikawa, Fuminori Mizukoshi, Hiroyuki Tsukagoshi, Yasutaka Ogawa, Takashi Nakada, Ai Kasuga, Tomoko Ogawa, Hajime Yokoi, Rieko Suzuki, Hideaki Shimizu, Satoko Kanazawa, Masayuki Oonuma, Nagano Environ, Kanako Nishizawa, Yuichiro Okamura, Asaka Ikegaya, Takaharu Maehata, Toshihiko Furuta, Masaya Nakazawa, Yoshihiro Yasui, Shinichiro Shibata, Tsuyoshi Kuzuguchi, Yasunori Tanaka, Hajime Kusuhara, Kayo Aoki, Sachi Hirata, Akiko Nagasao, Daiki Kanbayashi, Atsushi Kaida, Tatsuya Miyoshi, Miki Ogi, Taku Uemura, Shinya Kawanishi, Masaki Hiragakiuchi, Machi Inada, Takashi Nishiyama, Chika Tatsumi, Masako Hamano, Naoki Shigemoto, Fujii Yoshiki, Sachiko Murata, Yukari Terajima, Yumiko Kawakami, Yuki Ashizuka, Chinami Wasano, Misato Tachibana, Akiko Honda, Takashi Sakai, Kaori Nishizawa, Yu Matsuura, Mutsuyo Gokuden, Minori Oyama, Kenji Someya, Yuichiro Nakatsu, Maino Tahara, Kouji Sakai, Yukari Yamada, Kumi Ueno-Yamamoto, Yuki Tada, Tomoe Shimada, Kazuyo Yamashita, Hitomi Kinoshita, Takuri Takahashi, Kazutoshi Nakashima, Hajime Kamiya, Kiyosu Taniguchi, Yoshinori Yasui

Affiliations

¹ Department of Virology 3, National Institute of Infectious Diseases, Tokyo, Japan.
² Hokkaido Institute of Public Health, Sapporo, Japan.
³ Yamagata Prefectural Institute of Public Health, Yamagata, Japan.
⁴ Chiba Prefectural Institute of Public Health, Chiba, Japan.
⁵ Yokohama City Institute of Public Health, Yokohama, Japan.
⁶ Toyama Institute of Health, Imizu, Japan.
⁷ Aichi Prefectural Institute of Public Health, Nagoya, Japan.
⁸ Osaka Institute of Public Health, Osaka, Japan.
⁹ Tottori Prefectural Institute of Public Health and Environmental Science, Tottori, Japan.
¹⁰ Fukuoka Institute of Health and Environmental Sciences, Dazaifu, Japan.
¹¹ Okinawa Prefectural Institute of Health and Environment, Uruma, Japan.
¹² Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo, Japan.
¹³ Kawasaki City Institute for Public Health, Kawasaki, Japan.
¹⁴ Graduate School of Health Science, Gunma Paz University, Takasaki, Japan.
¹⁵ Department of Pediatrics, National Mie Hospital, Tsu, Japan.
¹⁶ Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
¹⁷ Yamaguchi Prefectural Institute of Public Health and Environment, Yamaguchi, Japan.

Abstract

Genotyping evidence that supports the interruption of endemic measles virus (MV) transmission is one of the essential criteria to be verified in achieving measles elimination. In Japan since 2014, MV genotype analyses have been performed for most of the measles cases in prefectural public health institutes nationwide. With this strong molecular epidemiological data, Japan was verified to have eliminated measles in March, 2015. However, even in the postelimination era, sporadic cases and small outbreaks of measles have been detected repeatedly in Japan. This study investigated the nationwide molecular epidemiology of MV between 2008 and 2017. The 891 strains in the total period between 2008 and 2017 belonged to seven genotypes (D5, D4, D9, H1, G3, B3, and D8) and 124 different MV sequence variants, based on the 450-nucleotide sequence region of the N gene (N450). The 311 MV strains in the postelimination era between 2015 and 2017 were classified into 1, 7, 8, and 32 different N450 sequence variants in D9, H1, B3, and D8 genotypes, respectively. Analysis of the detection period of the individual N450 sequence variants showed that the majority of MV strains were detected only for a short period. However, MV strains, MVs/Osaka.JPN/29.15/ [D8] and MVi/Hulu Langat.MYS/26.11/ [D8], which are named strains designated by World Health Organization (WHO), have been detected in many cases over 2 or 3 years between 2015 and 2017. The WHO-named strains have circulated worldwide, causing outbreaks in many countries. Epidemiological investigation revealed repeated importation of these WHO-named strains into Japan. To demonstrate the elimination status (interruption of endemic transmission) in situations with repeated importation of the same strains is challenging. Nevertheless, the detailed sequence analysis of individual MV strains and chronological analysis of these strains provided sufficient evidence to show that Japan has still maintained its measles elimination status in 2017.

Keywords: Japan; elimination; genotype; measles virus; molecular epidemiology.