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Allosteric activation of bacterial response regulators: the role of the cognate histidine kinase beyond phosphorylation.
Trajtenberg F, Albanesi D, Ruétalo N, Botti H, Mechaly AE, Nieves M, Aguilar PS, Cybulski L, Larrieux N, de Mendoza D, Buschiazzo A. Trajtenberg F, et al. Among authors: mechaly ae. mBio. 2014 Nov 18;5(6):e02105. doi: 10.1128/mBio.02105-14. mBio. 2014. PMID: 25406381 Free PMC article.
Potent and specific inhibition of glycosidases by small artificial binding proteins (affitins).
Correa A, Pacheco S, Mechaly AE, Obal G, Béhar G, Mouratou B, Oppezzo P, Alzari PM, Pecorari F. Correa A, et al. Among authors: mechaly ae. PLoS One. 2014 May 13;9(5):e97438. doi: 10.1371/journal.pone.0097438. eCollection 2014. PLoS One. 2014. PMID: 24823716 Free PMC article.
Conformational changes upon ligand binding in the essential class II fumarase Rv1098c from Mycobacterium tuberculosis.
Mechaly AE, Haouz A, Miras I, Barilone N, Weber P, Shepard W, Alzari PM, Bellinzoni M. Mechaly AE, et al. FEBS Lett. 2012 Jun 4;586(11):1606-11. doi: 10.1016/j.febslet.2012.04.034. Epub 2012 May 3. FEBS Lett. 2012. PMID: 22561013
Regulation of signaling directionality revealed by 3D snapshots of a kinase:regulator complex in action.
Trajtenberg F, Imelio JA, Machado MR, Larrieux N, Marti MA, Obal G, Mechaly AE, Buschiazzo A. Trajtenberg F, et al. Among authors: mechaly ae. Elife. 2016 Dec 12;5:e21422. doi: 10.7554/eLife.21422. Elife. 2016. PMID: 27938660 Free PMC article.
Structural Coupling between Autokinase and Phosphotransferase Reactions in a Bacterial Histidine Kinase.
Mechaly AE, Soto Diaz S, Sassoon N, Buschiazzo A, Betton JM, Alzari PM. Mechaly AE, et al. Structure. 2017 Jun 6;25(6):939-944.e3. doi: 10.1016/j.str.2017.04.011. Epub 2017 May 25. Structure. 2017. PMID: 28552574 Free article.
Conformational plasticity of the response regulator CpxR, a key player in Gammaproteobacteria virulence and drug-resistance.
Mechaly AE, Haouz A, Sassoon N, Buschiazzo A, Betton JM, Alzari PM. Mechaly AE, et al. J Struct Biol. 2018 Nov;204(2):165-171. doi: 10.1016/j.jsb.2018.08.001. Epub 2018 Aug 4. J Struct Biol. 2018. PMID: 30086390
Crystallization of FcpA from Leptospira, a novel flagellar protein that is essential for pathogenesis.
San Martin F, Mechaly AE, Larrieux N, Wunder EA Jr, Ko AI, Picardeau M, Trajtenberg F, Buschiazzo A. San Martin F, et al. Among authors: mechaly ae. Acta Crystallogr F Struct Biol Commun. 2017 Mar 1;73(Pt 3):123-129. doi: 10.1107/S2053230X17002096. Epub 2017 Feb 21. Acta Crystallogr F Struct Biol Commun. 2017. PMID: 28291747 Free PMC article.
Structural insights into the signalling mechanisms of two-component systems.
Jacob-Dubuisson F, Mechaly A, Betton JM, Antoine R. Jacob-Dubuisson F, et al. Among authors: mechaly a. Nat Rev Microbiol. 2018 Oct;16(10):585-593. doi: 10.1038/s41579-018-0055-7. Nat Rev Microbiol. 2018. PMID: 30008469 Review.
Structural Basis of Pullulanase Membrane Binding and Secretion Revealed by X-Ray Crystallography, Molecular Dynamics and Biochemical Analysis.
East A, Mechaly AE, Huysmans GHM, Bernarde C, Tello-Manigne D, Nadeau N, Pugsley AP, Buschiazzo A, Alzari PM, Bond PJ, Francetic O. East A, et al. Among authors: mechaly ae. Structure. 2016 Jan 5;24(1):92-104. doi: 10.1016/j.str.2015.10.023. Epub 2015 Dec 10. Structure. 2016. PMID: 26688215
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