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Nucleotide sequence of a human heart cDNA encoding the mitochondrial phosphate carrier.
Dolce V, Fiermonte G, Messina A, Palmieri F. Dolce V, et al. DNA Seq. 1991;2(2):133-5. doi: 10.3109/10425179109039683. DNA Seq. 1991. PMID: 1777677
We have isolated and characterized a full length cDNA clone encoding the precursor of the human heart mitochondrial phosphate carrier protein. ...The open reading frame encodes the mature protein consisting of 312 amino acids, preceded by a presequence of 49 amino a …
We have isolated and characterized a full length cDNA clone encoding the precursor of the human heart mitochondrial phosphate carrier …
The S-adenosyl-L-homocysteine hydrolase of Drosophila melanogaster: identification, deduced amino acid sequence and cytological localization of the structural gene.
Caggese C, Ragone G, Barsanti P, Moschetti R, Messina A, Massari S, Caizzi R. Caggese C, et al. Mol Gen Genet. 1997 Jan 27;253(4):492-8. doi: 10.1007/s004380050348. Mol Gen Genet. 1997. PMID: 9037110
Transcript analysis shows a single AdoHcyase mRNA present in unfertilized eggs, and, at a more or less constant level of expression, in all developmental stages tested, ranging from early embryos to adults. ...We discuss the hypothesis that a sequence that or …
Transcript analysis shows a single AdoHcyase mRNA present in unfertilized eggs, and, at a more or less constant level of expre …
Cloning and chromosomal localization of a cDNA encoding a mitochondrial porin from Drosophila melanogaster.
Messina A, Neri M, Perosa F, Caggese C, Marino M, Caizzi R, De Pinto V. Messina A, et al. FEBS Lett. 1996 Apr 8;384(1):9-13. doi: 10.1016/0014-5793(96)00268-2. FEBS Lett. 1996. PMID: 8797793
A subclone of 1T1, containing the porin-like sequence, was thus used as a probe for re-screening a cDNA library and several positive clones were plaque-purified. We present here the sequence of a 1363 bp cDNA encoding a protein of 279 amino acid
A subclone of 1T1, containing the porin-like sequence, was thus used as a probe for re-screening a cDNA library and sev
Cloning and sequencing of the rat cDNA encoding the mitochondrial 2-oxoglutarate carrier protein.
Dolce V, Messina A, Cambria A, Palmieri F. Dolce V, et al. DNA Seq. 1994;5(2):103-9. doi: 10.3109/10425179409039711. DNA Seq. 1994. PMID: 7703504
Using primers based on the bovine heart cDNA sequence, overlapping cDNA clones encoding the rat OGCP were isolated from total rat heart poly(A+) cDNA. ...The open reading frame encodes a protein consisting of 314 amino acids. The amino acid sequence of the rat 2-oxo …
Using primers based on the bovine heart cDNA sequence, overlapping cDNA clones encoding the rat OGCP were isolated from total rat heart poly …
Cloning and tissues expression of the pig CYP1B1 and CYP2J34.
Messina A, Siniscalco A, Puccinelli E, Gervasi PG, Longo V. Messina A, et al. Res Vet Sci. 2012 Jun;92(3):438-43. doi: 10.1016/j.rvsc.2011.04.012. Epub 2011 May 7. Res Vet Sci. 2012. PMID: 21550618
The open reading frames of pig CYP1B1 showed a higher sequence homology to bovine 1B1 (89%) than to dog 1B1 (88%) or to human 1B1 (85%). On the other hand, the coding sequence of pig CYP2J34 showed a similar homology (83-85%) to CYP2J of these species. From the subs …
The open reading frames of pig CYP1B1 showed a higher sequence homology to bovine 1B1 (89%) than to dog 1B1 (88%) or to human 1B1 (85 …
Molecular cloning and enzymatic characterization of sheep CYP2J.
Messina A, Nencioni S, Gervasi PG, Gotlinger KH, Schwartzman ML, Longo V. Messina A, et al. Xenobiotica. 2010 Feb;40(2):109-18. doi: 10.3109/00498250903410590. Xenobiotica. 2010. PMID: 20021200 Free PMC article.
The purified enzyme was catalytically active towards aminopyrine, all-trans-retinoic acid, and particularly arachidonic acid forming 20-HETE, 19-HETE, and 18-HETE (about 86% of the total) and 14,15-, 11,12-, 8,9-, and 5,6-EETs (cis-epoxyeicosatrienoic acids; about 14% of total), …
The purified enzyme was catalytically active towards aminopyrine, all-trans-retinoic acid, and particularly arachidonic acid forming 20-HETE …
Purification, molecular cloning, heterologous expression and characterization of pig CYP1A2.
Messina A, Chirulli V, Gervasi PG, Longo V. Messina A, et al. Xenobiotica. 2008 Dec;38(12):1453-70. doi: 10.1080/00498250802474437. Xenobiotica. 2008. PMID: 18949657
In a reconstituted system, this enzyme showed a good catalytic activity towards caffeine, acetanilide, and methoxyresorufin, all known markers of mammalian CYP1A2. Using 3'- and 5'-rapid amplification of coding DNA (cDNA) ends (RACE), we amplified from the liver RNA …
In a reconstituted system, this enzyme showed a good catalytic activity towards caffeine, acetanilide, and methoxyresorufin, a …
A New Splicing Isoform of Cacna2d4 Mimicking the Effects of c.2451insC Mutation in the Retina: Novel Molecular and Electrophysiological Insights.
Bacchi N, Messina A, Burtscher V, Dassi E, Provenzano G, Bozzi Y, Demontis GC, Koschak A, Denti MA, Casarosa S. Bacchi N, et al. Invest Ophthalmol Vis Sci. 2015 Jul;56(8):4846-56. doi: 10.1167/iovs.15-16410. Invest Ophthalmol Vis Sci. 2015. PMID: 26218913
RESULTS: Splicing analysis revealed the presence of a previously unknown splicing isoform of α2δ4 in the retina that truncates the gene open reading frame (ORF) in a similar way as the c.2451insC mutation. This isoform originates from alternative splicing of exon 25 …
RESULTS: Splicing analysis revealed the presence of a previously unknown splicing isoform of α2δ4 in the retina that truncates the ge …
Effects of the anticancer dehydrotarplatin on cytochrome P450 and antioxidant enzymes in male rat tissues.
Nannelli A, Messina A, Marini S, Trasciatti S, Longo V, Gervasi PG. Nannelli A, et al. Arch Toxicol. 2007 Jul;81(7):479-87. doi: 10.1007/s00204-007-0184-7. Epub 2007 Mar 16. Arch Toxicol. 2007. PMID: 17364183
DTP and, to a less extent, Triacid caused a depletion of plasmatic testosterone content and a down regulation in the liver of androgen dependent male specific CYP 2C11, but not of CYP 1A and 2E1, as determined by a significant decrease of 2alpha- and 1 …
DTP and, to a less extent, Triacid caused a depletion of plasmatic testosterone content and a down regulation in the li …
Expression and inducibility of CYP1A1, 1A2, 1B1 by β-naphthoflavone and CYP2B22, CYP3As by rifampicin in heart regions and coronary arteries of pig.
Messina A, Puccinelli E, Gervasi PG, Longo V. Messina A, et al. Res Vet Sci. 2013 Feb;94(1):77-83. doi: 10.1016/j.rvsc.2012.07.015. Epub 2012 Aug 11. Res Vet Sci. 2013. PMID: 22889553
Real-time PCR experiments and enzymatic or immunoblot assays showed that CYP1A1 was predominantly enhanced by βNF in a similar manner in all the heart regions, whereas antioxidant enzyme activity was not affected. ...These results obtained in vivo suggest that pig cardiac …
Real-time PCR experiments and enzymatic or immunoblot assays showed that CYP1A1 was predominantly enhanced by βNF in a similar manner …
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