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Stem cells derived from human fetal membranes display multilineage differentiation potential.
Ilancheran S, Michalska A, Peh G, Wallace EM, Pera M, Manuelpillai U. Ilancheran S, et al. Biol Reprod. 2007 Sep;77(3):577-88. doi: 10.1095/biolreprod.106.055244. Epub 2007 May 9. Biol Reprod. 2007. PMID: 17494917
Last, using flow cytometry we have shown that only a very small proportion of primary hAECs contain class IA and class II human leukocyte antigens (HLAs), consistent with a low risk of tissue rejection. ...These observations suggest that the term amnion, an abundant …
Last, using flow cytometry we have shown that only a very small proportion of primary hAECs contain class IA and class II human leuko …
Novel method for demonstrating nuclear contribution in mouse nuclear transfer.
Munsie M, Peura T, Michalska A, Trounson A, Mountford P. Munsie M, et al. Reprod Fertil Dev. 1998;10(7-8):633-7. doi: 10.1071/rd98098. Reprod Fertil Dev. 1998. PMID: 10612469
The use of donor nuclei isolated from lacZ transgenic mice offers a clear and simple method to demonstrate contribution in nuclear transfer embryos and offspring. ...
The use of donor nuclei isolated from lacZ transgenic mice offers a clear and simple method to demonstrate contribution in nuclear tr …
Isolation of pluripotent embryonic stem cells from reprogrammed adult mouse somatic cell nuclei.
Munsie MJ, Michalska AE, O'Brien CM, Trounson AO, Pera MF, Mountford PS. Munsie MJ, et al. Curr Biol. 2000 Aug 24;10(16):989-92. doi: 10.1016/s0960-9822(00)00648-5. Curr Biol. 2000. PMID: 10985386
Pluripotent human stem cells isolated from early embryos represent a potentially unlimited source of many different cell types for cell-based gene and tissue therapies [1-3]. ...Stem cells isolated from such a cloned embryo would be genetically identical to the pati …
Pluripotent human stem cells isolated from early embryos represent a potentially unlimited source of many different cell types for ce …
Method of derivation and differentiation of mouse embryonic stem cells generating synchronous neuronal networks.
Gazina EV, Morrisroe E, Mendis GDC, Michalska AE, Chen J, Nefzger CM, Rollo BN, Reid CA, Pera MF, Petrou S. Gazina EV, et al. J Neurosci Methods. 2018 Jan 1;293:53-58. doi: 10.1016/j.jneumeth.2017.08.018. Epub 2017 Aug 18. J Neurosci Methods. 2018. PMID: 28827162
Derivation and maintenance of human embryonic stem cell line on human adult skin fibroblast feeder cells in serum replacement medium.
Tecirlioglu RT, Nguyen L, Koh K, Trounson AO, Michalska AE. Tecirlioglu RT, et al. In Vitro Cell Dev Biol Anim. 2010 Apr;46(3-4):231-5. doi: 10.1007/s11626-010-9278-2. Epub 2010 Feb 23. In Vitro Cell Dev Biol Anim. 2010. PMID: 20178002
As a step towards this goal, we derived a new hES cell line (MISCES-01) on human adult skin fibroblasts as feeder cells using serum replacement (SR) medium. The MISCES-01 cells have a normal diploid karyotype (46XX), express markers of pluripotency (OCT4, GCT …
As a step towards this goal, we derived a new hES cell line (MISCES-01) on human adult skin fibroblasts as feeder cells using …
Generation of induced pluripotent stem cell lines from Friedreich ataxia patients.
Liu J, Verma PJ, Evans-Galea MV, Delatycki MB, Michalska A, Leung J, Crombie D, Sarsero JP, Williamson R, Dottori M, Pébay A. Liu J, et al. Stem Cell Rev Rep. 2011 Sep;7(3):703-13. doi: 10.1007/s12015-010-9210-x. Stem Cell Rev Rep. 2011. PMID: 21181307
It is caused by a trinucleotide (GAA) repeat expansion in the first intron of the FXN gene that results in reduced synthesis of FXN mRNA and its protein product, frataxin. ...As such, iPS cell lines derived from FRDA patients, following correction of the mutated gene, coul …
It is caused by a trinucleotide (GAA) repeat expansion in the first intron of the FXN gene that results in reduced synthesis of FXN m …
CD30 is a survival factor and a biomarker for transformed human pluripotent stem cells.
Herszfeld D, Wolvetang E, Langton-Bunker E, Chung TL, Filipczyk AA, Houssami S, Jamshidi P, Koh K, Laslett AL, Michalska A, Nguyen L, Reubinoff BE, Tellis I, Auerbach JM, Ording CJ, Looijenga LH, Pera MF. Herszfeld D, et al. Nat Biotechnol. 2006 Mar;24(3):351-7. doi: 10.1038/nbt1197. Epub 2006 Feb 26. Nat Biotechnol. 2006. PMID: 16501577
Here we show that CD30, a member of the tumor necrosis factor receptor superfamily, is expressed on transformed but not normal hES cells, and that CD30 expression protects hES cells against apoptosis....
Here we show that CD30, a member of the tumor necrosis factor receptor superfamily, is expressed on transformed but not normal hES ce …
Accelerated stem cell attachment to ultrafine grained titanium.
Estrin Y, Ivanova EP, Michalska A, Truong VK, Lapovok R, Boyd R. Estrin Y, et al. Acta Biomater. 2011 Feb;7(2):900-6. doi: 10.1016/j.actbio.2010.09.033. Epub 2010 Sep 29. Acta Biomater. 2011. PMID: 20887818
Surface characterization by a range of techniques showed that the main factor responsible for the observed acceleration of hMSC attachment and spreading on titanium due to grain refinement in the bulk is the attendant changes in surface topography on the nanoscale. These r …
Surface characterization by a range of techniques showed that the main factor responsible for the observed acceleration of hMSC attac …
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.
International Stem Cell Initiative, Adewumi O, Aflatoonian B, Ahrlund-Richter L, Amit M, Andrews PW, Beighton G, Bello PA, Benvenisty N, Berry LS, Bevan S, Blum B, Brooking J, Chen KG, Choo AB, Churchill GA, Corbel M, Damjanov I, Draper JS, Dvorak P, Emanuelsson K, Fleck RA, Ford A, Gertow K, Gertsenstein M, Gokhale PJ, Hamilton RS, Hampl A, Healy LE, Hovatta O, Hyllner J, Imreh MP, Itskovitz-Eldor J, Jackson J, Johnson JL, Jones M, Kee K, King BL, Knowles BB, Lako M, Lebrin F, Mallon BS, Manning D, Mayshar Y, McKay RD, Michalska AE, Mikkola M, Mileikovsky M, Minger SL, Moore HD, Mummery CL, Nagy A, Nakatsuji N, O'Brien CM, Oh SK, Olsson C, Otonkoski T, Park KY, Passier R, Patel H, Patel M, Pedersen R, Pera MF, Piekarczyk MS, Pera RA, Reubinoff BE, Robins AJ, Rossant J, Rugg-Gunn P, Schulz TC, Semb H, Sherrer ES, Siemen H, Stacey GN, Stojkovic M, Suemori H, Szatkiewicz J, Turetsky T, Tuuri T, van den Brink S, Vintersten K, Vuoristo S, Ward D, Weaver TA, Young LA, Zhang W. International Stem Cell Initiative, et al. Nat Biotechnol. 2007 Jul;25(7):803-16. doi: 10.1038/nbt1318. Epub 2007 Jun 17. Nat Biotechnol. 2007. PMID: 17572666
Derivation of phenotypically diverse neural culture from hESC by combining adherent and dissociation methods.
Liu Y, Antonic A, Yang X, Korte N, Lim K, Michalska AE, Dottori M, Howells DW. Liu Y, et al. J Neurosci Methods. 2018 Oct 1;308:286-293. doi: 10.1016/j.jneumeth.2018.07.005. Epub 2018 Jul 6. J Neurosci Methods. 2018. PMID: 30003885
NEW METHOD: We combined two commonly used differentiation methods to provide robust and reproducible cultures in which a mixture of primarily GABAergic and Glutamatergic neurons was obtained. ...CONCLUSION: Our method provides a novel, effective and robust neural cu …
NEW METHOD: We combined two commonly used differentiation methods to provide robust and reproducible cultures in which a mixture of p …
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