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A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery.
Katayama H, Kogure T, Mizushima N, Yoshimori T, Miyawaki A. Katayama H, et al. Among authors: miyawaki a. Chem Biol. 2011 Aug 26;18(8):1042-52. doi: 10.1016/j.chembiol.2011.05.013. Chem Biol. 2011. PMID: 21867919
We sought to develop a sensitive and quantitative technique capable of monitoring the entire flux of autophagy involving fusion of lysosomal membranes. We observed the accumulation inside lysosomal compartments of Keima, a coral-derived acid-stable fluorescent prote …
We sought to develop a sensitive and quantitative technique capable of monitoring the entire flux of autophagy involving fusion of ly …
Direct measurement of protein dynamics inside cells using a rationally designed photoconvertible protein.
Matsuda T, Miyawaki A, Nagai T. Matsuda T, et al. Among authors: miyawaki a. Nat Methods. 2008 Apr;5(4):339-45. doi: 10.1038/nmeth.1193. Epub 2008 Mar 16. Nat Methods. 2008. PMID: 18345008
We describe the development and application of a photoconvertible fluorescent protein, Phamret, that can be highlighted by UV light stimulation inducing a change in fluorescence emission from cyan fluorescent protein (CFP) to photoactivated GFP (PA-GFP). Phamret can …
We describe the development and application of a photoconvertible fluorescent protein, Phamret, that can be highlighted by UV light s …
Infrared spectroscopy reveals multi-step multi-timescale photoactivation in the photoconvertible protein archetype dronpa.
Laptenok SP, Gil AA, Hall CR, Lukacs A, Iuliano JN, Jones GA, Greetham GM, Donaldson P, Miyawaki A, Tonge PJ, Meech SR. Laptenok SP, et al. Among authors: miyawaki a. Nat Chem. 2018 Aug;10(8):845-852. doi: 10.1038/s41557-018-0073-0. Epub 2018 Jun 11. Nat Chem. 2018. PMID: 29892029 Free PMC article.
Following excitation of the trans chromophore, a ground-state primary product is formed within picoseconds. Surprisingly, the characteristic vibrational spectrum of the neutral cis isomer appears only after several tens of nanoseconds. Further fluctuations in protein struc …
Following excitation of the trans chromophore, a ground-state primary product is formed within picoseconds. Surprisingly, the charact …
Rational design of photoconvertible and biphotochromic fluorescent proteins for advanced microscopy applications.
Adam V, Moeyaert B, David CC, Mizuno H, Lelimousin M, Dedecker P, Ando R, Miyawaki A, Michiels J, Engelborghs Y, Hofkens J. Adam V, et al. Among authors: miyawaki a. Chem Biol. 2011 Oct 28;18(10):1241-51. doi: 10.1016/j.chembiol.2011.08.007. Chem Biol. 2011. PMID: 22035793
However, the spectroscopic properties of IrisFP are far from being optimal and its tetrameric organization complicates its use as a fusion tag. ...We present in particular NijiFP, a promising new fluorescent protein with photoconvertible and biphotochromic propertie …
However, the spectroscopic properties of IrisFP are far from being optimal and its tetrameric organization complicates its use as a f …
A theoretical study on the nature of on- and off-states of reversibly photoswitching fluorescent protein Dronpa: absorption, emission, protonation, and Raman.
Li X, Chung LW, Mizuno H, Miyawaki A, Morokuma K. Li X, et al. Among authors: miyawaki a. J Phys Chem B. 2010 Jan 21;114(2):1114-26. doi: 10.1021/jp909947c. J Phys Chem B. 2010. PMID: 19902912
A GFP-like fluorescent protein, Dronpa, which was engineered from a coral Pectiniidae, was found to display perfect photochromic properties; the fluorescent "on"-state and nonfluorescent "off"-state of Dronpa can be reversibly switched by irradiation of two differen
A GFP-like fluorescent protein, Dronpa, which was engineered from a coral Pectiniidae, was found to display perfect photochrom
The nephroblastoma overexpressed gene (NOV/ccn3) protein associates with Notch1 extracellular domain and inhibits myoblast differentiation via Notch signaling pathway.
Sakamoto K, Yamaguchi S, Ando R, Miyawaki A, Kabasawa Y, Takagi M, Li CL, Perbal B, Katsube K. Sakamoto K, et al. Among authors: miyawaki a. J Biol Chem. 2002 Aug 16;277(33):29399-405. doi: 10.1074/jbc.M203727200. Epub 2002 Jun 5. J Biol Chem. 2002. PMID: 12050162
We demonstrate a novel interaction of the nephroblastoma overexpressed gene (NOV), a member of the CCN gene family, with the Notch signaling pathway. ...These results indicate that NOV-Notch1 association exerts a positive effect on Notch signaling and consequ …
We demonstrate a novel interaction of the nephroblastoma overexpressed gene (NOV), a member of the CCN gene family, with the N …
Duration of fusion pore opening and the amount of hormone released are regulated by myosin II during kiss-and-run exocytosis.
Aoki R, Kitaguchi T, Oya M, Yanagihara Y, Sato M, Miyawaki A, Tsuboi T. Aoki R, et al. Among authors: miyawaki a. Biochem J. 2010 Aug 1;429(3):497-504. doi: 10.1042/BJ20091839. Biochem J. 2010. PMID: 20528772
In contrast, overexpression of a dominant-negative form of RLC shortened the release kinetics. Using spH (synapto-pHluorin), a green fluorescent protein-based pH sensor inside the vesicles, we confirmed that the modulation of the release kinetics by myosin II is due …
In contrast, overexpression of a dominant-negative form of RLC shortened the release kinetics. Using spH (synapto-pHluorin), a
Simultaneous dual-excitation ratiometry using orthogonal linear polarized lights.
Fukano T, Shimozono S, Miyawaki A. Fukano T, et al. Among authors: miyawaki a. Biochem Biophys Res Commun. 2004 Apr 23;317(1):77-83. doi: 10.1016/j.bbrc.2004.03.009. Biochem Biophys Res Commun. 2004. PMID: 15047150
Here, we present a novel but simple dual-excitation ratiometric method which overcomes this problem. By the use of our home-made illuminator, each sample is illuminated by two orthogonal linear polarized lights of different wavelengths. ...
Here, we present a novel but simple dual-excitation ratiometric method which overcomes this problem. By the use of our home-made illu …
Development of probes for cellular functions using fluorescent proteins and fluorescence resonance energy transfer.
Miyawaki A. Miyawaki A. Annu Rev Biochem. 2011;80:357-73. doi: 10.1146/annurev-biochem-072909-094736. Annu Rev Biochem. 2011. PMID: 21529159 Review.
The potential benefits and limitations of a variety of features in the technologies using fluorescent proteins and FRET are discussed....
The potential benefits and limitations of a variety of features in the technologies using fluorescent proteins and FRET are discussed …
Photoconvertible Behavior of LSSmOrange Applicable for Single Emission Band Optical Highlighting.
De Keersmaecker H, Fron E, Rocha S, Kogure T, Miyawaki A, Hofkens J, Mizuno H. De Keersmaecker H, et al. Among authors: miyawaki a. Biophys J. 2016 Sep 6;111(5):1014-25. doi: 10.1016/j.bpj.2016.07.033. Biophys J. 2016. PMID: 27602729 Free PMC article.
LSSmOrange behaves as a freely diffusing protein in living cells, enabling the use of the protein as a fluorescence tag for studies of protein dynamics. ...Furthermore, by using a femtosecond laser at 850 nm, we showed that a two-photon process could e …
LSSmOrange behaves as a freely diffusing protein in living cells, enabling the use of the protein as a fluorescence tag for st …
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