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Functional metagenomics for enzyme discovery: challenges to efficient screening.
Uchiyama T, Miyazaki K. Uchiyama T, et al. Curr Opin Biotechnol. 2009 Dec;20(6):616-22. doi: 10.1016/j.copbio.2009.09.010. Epub 2009 Oct 21. Curr Opin Biotechnol. 2009. PMID: 19850467 Review.
Creating random mutagenesis libraries using megaprimer PCR of whole plasmid.
Miyazaki K, Takenouchi M. Miyazaki K, et al. Biotechniques. 2002 Nov;33(5):1033-4, 1036-8. doi: 10.2144/02335st03. Biotechniques. 2002. PMID: 12449380
Random DNA fragmentation with endonuclease V: application to DNA shuffling.
Miyazaki K. Miyazaki K. Nucleic Acids Res. 2002 Dec 15;30(24):e139. doi: 10.1093/nar/gnf139. Nucleic Acids Res. 2002. PMID: 12490730 Free PMC article.
A hyperthermophilic laccase from Thermus thermophilus HB27.
Miyazaki K. Miyazaki K. Extremophiles. 2005 Dec;9(6):415-25. doi: 10.1007/s00792-005-0458-z. Epub 2005 Jul 6. Extremophiles. 2005. PMID: 15999224
Thermal stabilization of Bacillus subtilis family-11 xylanase by directed evolution.
Miyazaki K, Takenouchi M, Kondo H, Noro N, Suzuki M, Tsuda S. Miyazaki K, et al. J Biol Chem. 2006 Apr 14;281(15):10236-42. doi: 10.1074/jbc.M511948200. Epub 2006 Feb 8. J Biol Chem. 2006. PMID: 16467302
The structural basis for the exo-mode of action in GH74 oligoxyloglucan reducing end-specific cellobiohydrolase.
Yaoi K, Kondo H, Hiyoshi A, Noro N, Sugimoto H, Tsuda S, Mitsuishi Y, Miyazaki K. Yaoi K, et al. J Mol Biol. 2007 Jun 29;370(1):53-62. doi: 10.1016/j.jmb.2007.04.035. Epub 2007 Apr 19. J Mol Biol. 2007. PMID: 17498741
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