Investigation of the molecular mechanism of ICAN, a novel gene amplification method.
Uemori T, Mukai H, Takeda O, Moriyama M, Sato Y, Hokazono S, Takatsu N, Asada K, Kato I.
Uemori T, et al. Among authors: mukai h.
J Biochem. 2007 Aug;142(2):283-92. doi: 10.1093/jb/mvm137. Epub 2007 Aug 24.
J Biochem. 2007.
PMID: 17720719
Isothermal and Chimeric primer-initiated Amplification of Nucleic acids (ICAN) allows the amplification of target DNA under isothermal conditions at around 55 degrees C using only a pair of 5'-DNA-RNA-3' chimeric primers, thermostable RNaseH and a DNA polymerase with strand-displ …
Isothermal and Chimeric primer-initiated Amplification of Nucleic acids (ICAN) allows the amplification of target DNA under isothermal condi …