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Self-Assembling Scaffolds Supported Long-Term Growth of Human Primed Embryonic Stem Cells and Upregulated Core and Naïve Pluripotent Markers.
McKee C, Brown C, Chaudhry GR. McKee C, et al. Cells. 2019 Dec 16;8(12):1650. doi: 10.3390/cells8121650. Cells. 2019. PMID: 31888235 Free PMC article.
The maintenance and expansion of human embryonic stem cells (ESCs) in two-dimensional (2-D) culture is technically challenging, requiring routine manipulation and passaging. ...Three-dimensional (3-D) self-assembling scaffold-grown cells displayed an u …
The maintenance and expansion of human embryonic stem cells (ESCs) in two-dimensional (2-D) culture is technically chal …
In vitro culture and phenotypic and molecular characterization of gastric stem cells from human stomach.
Garcia M, Chomel JC, Mustapha P, Tran CT, Garnier M, Paris I, Quellard N, Godet J, Cremniter J, Bennaceur-Griscelli A, Lecron JC, Turhan AG, Burucoa C, Bodet C. Garcia M, et al. Helicobacter. 2017 Apr;22(2). doi: 10.1111/hel.12351. Epub 2016 Sep 4. Helicobacter. 2017. PMID: 27592706
METHODS: We describe an original protocol for culture of gastric stem/progenitor cells from adult human stomach. The molecular characteristics of cells were studied using TaqMan low-density array and qRT-PCR analyses using the well-characterized H1 and H9
METHODS: We describe an original protocol for culture of gastric stem/progenitor cells from adult human stomach. The molecular …
Assessment of the Potential of CDK2 Inhibitor NU6140 to Influence the Expression of Pluripotency Markers NANOG, OCT4, and SOX2 in 2102Ep and H9 Cells.
Kallas A, Pook M, Trei A, Maimets T. Kallas A, et al. Int J Cell Biol. 2014;2014:280638. doi: 10.1155/2014/280638. Epub 2014 Nov 17. Int J Cell Biol. 2014. PMID: 25477962 Free PMC article.
In this study we investigated the effects of the small molecular agent NU6140 (inhibits CDK2 and cyclin A interaction) on human embryonic stem (hES) cells and embryonal carcinoma-derived (hEC) cells via the expression of transcription factors responsib …
In this study we investigated the effects of the small molecular agent NU6140 (inhibits CDK2 and cyclin A interaction) on human embryonic
Lovastatin Decreases the Expression of CD133 and Influences the Differentiation Potential of Human Embryonic Stem Cells.
Kallas-Kivi A, Trei A, Maimets T. Kallas-Kivi A, et al. Stem Cells Int. 2016;2016:1580701. doi: 10.1155/2016/1580701. Epub 2016 May 10. Stem Cells Int. 2016. PMID: 27247576 Free PMC article.
In this study, we investigated the effects of lovastatin on the differentiation potential of human embryonic stem (hES) cells (H9 cell line). ...We found that lovastatin treatment delayed NANOG downregulation during ectodermal and endodermal dif …
In this study, we investigated the effects of lovastatin on the differentiation potential of human embryonic stem (hES) cel
Hair follicle-derived mesenchymal cells support undifferentiated growth of embryonic stem cells.
Coelho de Oliveira VC, Silva Dos Santos D, Vairo L, Kasai Brunswick TH, Pimentel LAS, Carvalho AB, Campos de Carvalho AC, Goldenberg RCDS. Coelho de Oliveira VC, et al. Exp Ther Med. 2017 May;13(5):1779-1788. doi: 10.3892/etm.2017.4195. Epub 2017 Mar 8. Exp Ther Med. 2017. PMID: 28565767 Free PMC article.
The aim of the present study was to investigate whether feeder layers composed of human hair follicle-derived mesenchymal stem cells (hHFDCs) are able to support human embryonic stem cells (hESCs). hHFDCs and mouse embryonic fibroblasts ( …
The aim of the present study was to investigate whether feeder layers composed of human hair follicle-derived mesenchymal stem cel
Primitive cardiac cells from human embryonic stem cells.
Hudson J, Titmarsh D, Hidalgo A, Wolvetang E, Cooper-White J. Hudson J, et al. Stem Cells Dev. 2012 Jun 10;21(9):1513-23. doi: 10.1089/scd.2011.0254. Epub 2011 Nov 9. Stem Cells Dev. 2012. PMID: 21933026
In this study, we have developed a differentiation protocol that minimizes the need for specific human embryonic stem cell (hESC) line optimization. ...Compared with bulk cultures, single-cell cultures comprised larger fractions of TG30(hi)/OCT4(hi) cells, co …
In this study, we have developed a differentiation protocol that minimizes the need for specific human embryonic stem cell (hE …
Induction of early neural precursors and derivation of tripotent neural stem cells from human pluripotent stem cells under xeno-free conditions.
Nguyen HX, Nekanti U, Haus DL, Funes G, Moreno D, Kamei N, Cummings BJ, Anderson AJ. Nguyen HX, et al. J Comp Neurol. 2014 Aug 15;522(12):2767-83. doi: 10.1002/cne.23604. Epub 2014 Apr 25. J Comp Neurol. 2014. PMID: 24715528 Free article.
Human embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC) can differentiate into many cell types and are important for regenerative medicine; however, further work is needed to reliably differentiate hESC and hiPSC into neural …
Human embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC) can differentiate into many …
Effect of antibiotics against Mycoplasma sp. on human embryonic stem cells undifferentiated status, pluripotency, cell viability and growth.
Romorini L, Riva DA, Blüguermann C, Videla Richardson GA, Scassa ME, Sevlever GE, Miriuka SG. Romorini L, et al. PLoS One. 2013 Jul 30;8(7):e70267. doi: 10.1371/journal.pone.0070267. Print 2013. PLoS One. 2013. PMID: 23936178 Free PMC article.
Human embryonic stem cells (hESCs) are self-renewing pluripotent cells that can differentiate into specialized cells and hold great promise as models for human development and disease studies, cell-replacement therapies, drug discovery and in vi …
Human embryonic stem cells (hESCs) are self-renewing pluripotent cells that can differentiate into specialized …
Establishment of clinically compliant human embryonic stem cells in an autologous feeder-free system.
Fu X, Toh WS, Liu H, Lu K, Li M, Cao T. Fu X, et al. Tissue Eng Part C Methods. 2011 Sep;17(9):927-37. doi: 10.1089/ten.TEC.2010.0735. Epub 2011 Jun 20. Tissue Eng Part C Methods. 2011. PMID: 21561302
Applications of human embryonic stem cells (hESCs) are limited by the use of mouse embryonic fibroblasts feeder and animal-derived components during culture. ...Autologous H9 ebF (feeder cells derived from outgrowth of embryoid body [EB] …
Applications of human embryonic stem cells (hESCs) are limited by the use of mouse embryonic fibroblasts feeder …
Hematopoietic and endothelial differentiation of human induced pluripotent stem cells.
Choi KD, Yu J, Smuga-Otto K, Salvagiotto G, Rehrauer W, Vodyanik M, Thomson J, Slukvin I. Choi KD, et al. Stem Cells. 2009 Mar;27(3):559-67. doi: 10.1634/stemcells.2008-0922. Stem Cells. 2009. PMID: 19259936 Free PMC article.
Induced pluripotent stem cells (iPSCs) provide an unprecedented opportunity for modeling of human diseases in vitro, as well as for developing novel approaches for regenerative therapy based on immunologically compatible cells. In this study, we employed an O …
Induced pluripotent stem cells (iPSCs) provide an unprecedented opportunity for modeling of human diseases in vitro, as well a …
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