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Correlated waves of actin filaments and PIP3 in Dictyostelium cells.
Asano Y, Nagasaki A, Uyeda TQ. Asano Y, et al. Cell Motil Cytoskeleton. 2008 Dec;65(12):923-34. doi: 10.1002/cm.20314. Cell Motil Cytoskeleton. 2008. PMID: 18814278
Chemotaxis-deficient amiB-null mutant Dictyostelium cells show two distinct movements: (1) they extend protrusions randomly without net displacements; (2) they migrate persistently and unidirectionally in a keratocyte-like manner. ...
Chemotaxis-deficient amiB-null mutant Dictyostelium cells show two distinct movements: (1) they extend protrusions randomly without net disp …
A genome editing vector that enables easy selection and identification of knockout cells.
Nagasaki A, Kato Y, Meguro K, Yamagishi A, Nakamura C, Uyeda TQP. Nagasaki A, et al. Plasmid. 2018 Jun;98:37-44. doi: 10.1016/j.plasmid.2018.08.005. Epub 2018 Sep 6. Plasmid. 2018. PMID: 30196057
The Position of the GFP Tag on Actin Affects the Filament Formation in Mammalian Cells.
Nagasaki A, T Kijima S, Yumoto T, Imaizumi M, Yamagishi A, Kim H, Nakamura C, Q P Uyeda T. Nagasaki A, et al. Cell Struct Funct. 2017;42(2):131-140. doi: 10.1247/csf.17016. Cell Struct Funct. 2017. PMID: 28855440 Free article.
Phospholipase D is essential for keratocyte-like migration of NBT-II cells.
Nagasaki A, Inotsume K, Kanada M, Uyeda TQ. Nagasaki A, et al. Cell Struct Funct. 2008;33(1):27-33. doi: 10.1247/csf.07042. Epub 2008 Mar 13. Cell Struct Funct. 2008. PMID: 18344600
NBT-II cells on collagen-coated substrates move rapidly and persistently, maintaining a semi-circular shape with a large lamellipodium, in a manner similar to fish keratocytes. ...
NBT-II cells on collagen-coated substrates move rapidly and persistently, maintaining a semi-circular shape with a large lamel …
A novel shRNA vector that enables rapid selection and identification of knockdown cells.
Nagasaki A, Kanada M, Uyeda TQ. Nagasaki A, et al. Plasmid. 2007 Sep;58(2):190-4. doi: 10.1016/j.plasmid.2007.01.006. Epub 2007 Aug 3. Plasmid. 2007. PMID: 17688947
Small interference RNA (siRNA) is a powerful tool for disrupting expression of specific genes in a variety of cells. We have developed a vector, piMARK, which mediates expression of both small hairpin RNA (shRNA) and the blasticidin resistance (Bsr) protein f …
Small interference RNA (siRNA) is a powerful tool for disrupting expression of specific genes in a variety of cells. We have d …
DWWA, a novel protein containing two WW domains and an IQ motif, is required for scission of the residual cytoplasmic bridge during cytokinesis in Dictyostelium.
Nagasaki A, Uyeda TQ. Nagasaki A, et al. Mol Biol Cell. 2004 Feb;15(2):435-46. doi: 10.1091/mbc.e03-05-0329. Epub 2003 Oct 31. Mol Biol Cell. 2004. PMID: 14595117 Free PMC article.
We have identified a novel gene, dwwA, which is required for cytokinesis of Dictyostelium cells on solid surfaces. Its product, Dd WW domain containing protein A (DWWA), contains several motifs, including two WW domains, an IQ motif, a C2 domain, and a
We have identified a novel gene, dwwA, which is required for cytokinesis of Dictyostelium cells on solid surfaces. Its product, Dd WW …
Novel myosin heavy chain kinase involved in disassembly of myosin II filaments and efficient cleavage in mitotic dictyostelium cells.
Nagasaki A, Itoh G, Yumura S, Uyeda TQ. Nagasaki A, et al. Mol Biol Cell. 2002 Dec;13(12):4333-42. doi: 10.1091/mbc.e02-04-0228. Mol Biol Cell. 2002. PMID: 12475956 Free PMC article.
We have cloned a full-length cDNA encoding a novel myosin II heavy chain kinase (mhckC) from Dictyostelium. Like other members of the myosin heavy chain kinase family, the mhckC gene product, MHCK C, has a kinase domain in its N-terminal half and six WD repea …
We have cloned a full-length cDNA encoding a novel myosin II heavy chain kinase (mhckC) from Dictyostelium. Like other members …
Genetic and morphological evidence for two parallel pathways of cell-cycle-coupled cytokinesis in Dictyostelium.
Nagasaki A, de Hostos EL, Uyeda TQ. Nagasaki A, et al. J Cell Sci. 2002 May 15;115(Pt 10):2241-51. J Cell Sci. 2002. PMID: 11973364
Myosin-II-null cells of Dictyostelium discoideum cannot divide in suspension, consistent with the dogma that myosin II drives constriction of the cleavage furrow and, consequently, cytokinesis (cytokinesis A). ...These double mutants multiplied mainly by cytokinesis C, …
Myosin-II-null cells of Dictyostelium discoideum cannot divide in suspension, consistent with the dogma that myosin II drives constriction o …
Desalted deep sea water increases transformation and homologous recombination efficiencies in Dictyostelium discoideum.
Kuwayama H, Nagasaki A. Kuwayama H, et al. J Mol Microbiol Biotechnol. 2008;14(4):157-62. doi: 10.1159/000107371. Epub 2007 Aug 13. J Mol Microbiol Biotechnol. 2008. PMID: 17693704
By virtue of its relatively high transformation efficiency and a small haploid genome, this organism has proven to be advantageous for characterizing gene functions. However, a much higher transformation efficiency is required as one of the prerequisites for unravel …
By virtue of its relatively high transformation efficiency and a small haploid genome, this organism has proven to be advantageous fo …
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