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Nanopore sequencing and assembly of a human genome with ultra-long reads.
Jain M, Koren S, Miga KH, Quick J, Rand AC, Sasani TA, Tyson JR, Beggs AD, Dilthey AT, Fiddes IT, Malla S, Marriott H, Nieto T, O'Grady J, Olsen HE, Pedersen BS, Rhie A, Richardson H, Quinlan AR, Snutch TP, Tee L, Paten B, Phillippy AM, Simpson JT, Loman NJ, Loose M. Jain M, et al. Nat Biotechnol. 2018 Apr;36(4):338-345. doi: 10.1038/nbt.4060. Epub 2018 Jan 29. Nat Biotechnol. 2018. PMID: 29431738 Free PMC article.
We developed a protocol to generate ultra-long reads (N50 > 100 kb, read lengths up to 882 kb). Incorporating an additional 5 coverage of these ultra-long reads more than doubled the assembly contiguity (NG50 6.4 Mb). ...Ultr
We developed a protocol to generate ultra-long reads (N50 > 100 kb, read lengths up to 882 kb). Incorporating an add …
Long-read sequencing in deciphering human genetics to a greater depth.
Midha MK, Wu M, Chiu KP. Midha MK, et al. Hum Genet. 2019 Dec;138(11-12):1201-1215. doi: 10.1007/s00439-019-02064-y. Epub 2019 Sep 19. Hum Genet. 2019. PMID: 31538236 Review.
Long reads (LRs) are considered as the most advantageous feature of SMS shared by both PacBio and ONT to distinguish SMS from next-generation sequencing (NGS, or the second-generation sequencing) and Sanger sequencing (the first-generation se
Long reads (LRs) are considered as the most advantageous feature of SMS shared by both PacBio and ONT to distinguish SMS from
Hybrid assembly of ultra-long Nanopore reads augmented with 10x-Genomics contigs: Demonstrated with a human genome.
Ma ZS, Li L, Ye C, Peng M, Zhang YP. Ma ZS, et al. Genomics. 2019 Dec;111(6):1896-1901. doi: 10.1016/j.ygeno.2018.12.013. Epub 2018 Dec 27. Genomics. 2019. PMID: 30594583 Free article.
The 3rd generation of sequencing (3GS) technologies generate ultra-long reads (up to 1 Mb), which makes it possible to eliminate gaps and effectively resolve repeats in genome assembly. ...Using a human genome as an example, …
The 3rd generation of sequencing (3GS) technologies generate ultra-long reads (up to 1 Mb), which makes it possi …
MinION nanopore sequencing and assembly of a complete human papillomavirus genome.
Brancaccio RN, Robitaille A, Dutta S, Rollison DE, Tommasino M, Gheit T. Brancaccio RN, et al. J Virol Methods. 2021 Aug;294:114180. doi: 10.1016/j.jviromet.2021.114180. Epub 2021 May 7. J Virol Methods. 2021. PMID: 33965458 Free PMC article.
BACKGROUND: The MinION sequencer belongs to the third generation of sequencing technology that allows for the generation of ultra-long reads, representing a potentially more effective approach to characterize entire viral genome sequences than o …
BACKGROUND: The MinION sequencer belongs to the third generation of sequencing technology that allows for the generation of ultra
Scalable long read self-correction and assembly polishing with multiple sequence alignment.
Morisse P, Marchet C, Limasset A, Lecroq T, Lefebvre A. Morisse P, et al. Sci Rep. 2021 Jan 12;11(1):761. doi: 10.1038/s41598-020-80757-5. Sci Rep. 2021. PMID: 33436980 Free PMC article.
Third-generation sequencing technologies allow to sequence long reads of tens of kbp, that are expected to solve various problems. ...CONSENT compares well to the state-of-the-art, and performs better on real Oxford Nanopore data. Specifically, CONSENT …
Third-generation sequencing technologies allow to sequence long reads of tens of kbp, that are expected to solve variou …