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Inducible gene expression mediated by a repressor-operator system isolated from Lactococcus lactis bacteriophage r1t.
Nauta A, van Sinderen D, Karsens H, Smit E, Venema G, Kok J. Nauta A, et al. Mol Microbiol. 1996 Mar;19(6):1331-41. doi: 10.1111/j.1365-2958.1996.tb02477.x. Mol Microbiol. 1996. PMID: 8730874
To study possible transcriptional regulation of the region, a lacZ translational fusion with an open reading frame following tec was constructed. ...In non-induced cells, promoter activity was repressed by Rro, as a frameshift mutation in rro resulted in constitutiv …
To study possible transcriptional regulation of the region, a lacZ translational fusion with an open reading frame following tec was …
The Lactococcus lactis CodY regulon: identification of a conserved cis-regulatory element.
den Hengst CD, van Hijum SA, Geurts JM, Nauta A, Kok J, Kuipers OP. den Hengst CD, et al. Among authors: nauta a. J Biol Chem. 2005 Oct 7;280(40):34332-42. doi: 10.1074/jbc.M502349200. Epub 2005 Jul 21. J Biol Chem. 2005. PMID: 16040604
CodY of Lactococcus lactis MG1363 is a transcriptional regulator that represses the expression of several genes encoding proteins of the proteolytic system. ...Evidence is presented that this 15-bp cis-sequence, AATTTTCWGAAAATT, serves as a high affinity binding sit …
CodY of Lactococcus lactis MG1363 is a transcriptional regulator that represses the expression of several genes encoding proteins of …
Probing direct interactions between CodY and the oppD promoter of Lactococcus lactis.
den Hengst CD, Curley P, Larsen R, Buist G, Nauta A, van Sinderen D, Kuipers OP, Kok J. den Hengst CD, et al. Among authors: nauta a. J Bacteriol. 2005 Jan;187(2):512-21. doi: 10.1128/JB.187.2.512-521.2005. J Bacteriol. 2005. PMID: 15629923 Free PMC article.
CodY of Lactococcus lactis MG1363 is a transcriptional regulator that represses the expression of several genes encoding proteins of the proteolytic system. ...Random and site-directed mutagenesis of the upstream region of oppD yielded variants that were derepressed in …
CodY of Lactococcus lactis MG1363 is a transcriptional regulator that represses the expression of several genes encoding proteins of …
Functional analysis of the gene cluster involved in production of the bacteriocin circularin A by Clostridium beijerinckii ATCC 25752.
Kemperman R, Jonker M, Nauta A, Kuipers OP, Kok J. Kemperman R, et al. Among authors: nauta a. Appl Environ Microbiol. 2003 Oct;69(10):5839-48. doi: 10.1128/aem.69.10.5839-5848.2003. Appl Environ Microbiol. 2003. PMID: 14532033 Free PMC article.
A region of 12 kb flanking the structural gene of the cyclic antibacterial peptide circularin A of Clostridium beijerinckii ATCC 25752 was sequenced, and the putative proteins involved in the production and secretion of circularin A were identified. ...Additi
A region of 12 kb flanking the structural gene of the cyclic antibacterial peptide circularin A of Clostridium beijerinckii AT
Characterization of the putative replisome organizer of the lactococcal bacteriophage r1t.
Zúñiga M, Franke-Fayard B, Venema G, Kok J, Nauta A. Zúñiga M, et al. Among authors: nauta a. J Virol. 2002 Oct;76(20):10234-44. doi: 10.1128/jvi.76.20.10234-10244.2002. J Virol. 2002. PMID: 12239299 Free PMC article.
Gel retardation analysis using various r1t DNA fragments indicates that Pro11 specifically binds to a sequence located within its cognate gene. ...We propose a model for the binding of Pro11 to its target sites that may account for the torsion strain required for st …
Gel retardation analysis using various r1t DNA fragments indicates that Pro11 specifically binds to a sequence located within its cog …
Identification and characterization of two novel clostridial bacteriocins, circularin A and closticin 574.
Kemperman R, Kuipers A, Karsens H, Nauta A, Kuipers O, Kok J. Kemperman R, et al. Among authors: nauta a. Appl Environ Microbiol. 2003 Mar;69(3):1589-97. doi: 10.1128/aem.69.3.1589-1597.2003. Appl Environ Microbiol. 2003. PMID: 12620847 Free PMC article.
Circularin A is produced by Clostridium beijerinckii ATCC 25752 as a prepeptide of 72 amino acids. Cleavage of the prepeptide between the third leucine and fourth valine residues followed by a head-to-tail ligation between the N and C termini creates a
Circularin A is produced by Clostridium beijerinckii ATCC 25752 as a prepeptide of 72 amino acids. Cleavage of the prepeptide …
Biocidal Inactivation of Lactococcus lactis Bacteriophages: Efficacy and Targets of Commonly Used Sanitizers.
Hayes S, Murphy J, Mahony J, Lugli GA, Ventura M, Noben JP, Franz CM, Neve H, Nauta A, Van Sinderen D. Hayes S, et al. Among authors: nauta a. Front Microbiol. 2017 Feb 2;8:107. doi: 10.3389/fmicb.2017.00107. eCollection 2017. Front Microbiol. 2017. PMID: 28210242 Free PMC article.
Sanitization and disinfection using purpose-made biocidal solutions is a critical step in controlling phage contamination in such dairy processing plants. ...Furthermore, rather than possessing resistance to specific biocides or biocide types, biocide-resistant phages tend …
Sanitization and disinfection using purpose-made biocidal solutions is a critical step in controlling phage contamination in such dai …
Functional carbohydrate binding modules identified in evolved dits from siphophages infecting various Gram-positive bacteria.
Hayes S, Vincentelli R, Mahony J, Nauta A, Ramond L, Lugli GA, Ventura M, van Sinderen D, Cambillau C. Hayes S, et al. Among authors: nauta a. Mol Microbiol. 2018 Dec;110(5):777-795. doi: 10.1111/mmi.14124. Epub 2018 Oct 30. Mol Microbiol. 2018. PMID: 30204278
In the current report, using bioinformatic analysis we identified a conserved carbohydrate binding module (CBM) among many of the virion baseplate Dit components, in addition to the CBM present in the 'classical' receptor binding proteins (RBPs). ...The omnipresence of CBM …
In the current report, using bioinformatic analysis we identified a conserved carbohydrate binding module (CBM) among many of the vir …
Design of thermolabile bacteriophage repressor mutants by comparative molecular modeling.
Nauta A, van den Burg B, Karsens H, Venema G, Kok J. Nauta A, et al. Nat Biotechnol. 1997 Oct;15(10):980-3. doi: 10.1038/nbt1097-980. Nat Biotechnol. 1997. PMID: 9335049
Rro residues presumed to stabilize a nonhomologous but structurally conserved hydrophobic pocket, which was shown to be important for thermostability of the Escherichia coli bacteriophage lambda repressor CI, were randomized. ...
Rro residues presumed to stabilize a nonhomologous but structurally conserved hydrophobic pocket, which was shown to be important for …
Autolysis of Lactococcus lactis caused by induced overproduction of its major autolysin, AcmA.
Buist G, Karsens H, Nauta A, van Sinderen D, Venema G, Kok J. Buist G, et al. Among authors: nauta a. Appl Environ Microbiol. 1997 Jul;63(7):2722-8. doi: 10.1128/AEM.63.7.2722-2728.1997. Appl Environ Microbiol. 1997. PMID: 9212419 Free PMC article.
The optical density of a culture of lactococcus lactis MG1363 was reduced more than 60% during prolonged stationary phase. Reduction in optical density (autolysis) was almost absent in a culture of an isogenic mutant containing a deletion in the major autolys …
The optical density of a culture of lactococcus lactis MG1363 was reduced more than 60% during prolonged stationary phase. Reduction …
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